Cloned (Comment) | Organism |
---|---|
the gene is artificially synthesized for optimal recombinant expression in Escherichia coli strain Tuner (DE3) | Thermosynechococcus vestitus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.033 | - |
ADP-glucose | pH 7.0, 37°C, recombinant enzyme | Thermosynechococcus vestitus | |
0.18 | - |
ADP | pH 7.0, 37°C, recombinant enzyme | Thermosynechococcus vestitus | |
0.9 | - |
CDP | pH 7.0, 37°C, recombinant enzyme | Thermosynechococcus vestitus | |
1.1 | - |
GDP | pH 7.0, 37°C, recombinant enzyme | Thermosynechococcus vestitus | |
1.3 | - |
UDP | pH 7.0, 37°C, recombinant enzyme | Thermosynechococcus vestitus | |
1.4 | - |
TDP | pH 7.0, 37°C, recombinant enzyme | Thermosynechococcus vestitus | |
1.7 | - |
UDP-glucose | pH 7.0, 37°C, recombinant enzyme | Thermosynechococcus vestitus | |
5.6 | - |
D-fructose | pH 7.0, 37°C, recombinant enzyme, with ADP-glucose | Thermosynechococcus vestitus | |
12 | - |
D-fructose | pH 7.0, 37°C, recombinant enzyme, with UDP-glucose | Thermosynechococcus vestitus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Thermosynechococcus vestitus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
95000 | - |
4 * 95000, recombinant enzyme, SDS-PAGE | Thermosynechococcus vestitus |
341000 | - |
recombinant enzyme, gel filtration | Thermosynechococcus vestitus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
NDP-glucose + D-fructose | Thermosynechococcus vestitus | - |
NDP + sucrose | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermosynechococcus vestitus | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ADP-glucose + D-fructose | - |
Thermosynechococcus vestitus | ADP + sucrose | ADP is the best acceptor substrate in sucrose cleavage | r | |
CDP-glucose + D-fructose | - |
Thermosynechococcus vestitus | CDP + sucrose | - |
r | |
GDP-glucose + D-fructose | - |
Thermosynechococcus vestitus | GDP + sucrose | - |
r | |
additional information | unique nucleotide specificity of the sucrose synthase from Thermosynechococcus elongatus | Thermosynechococcus vestitus | ? | - |
? | |
NDP-glucose + D-fructose | - |
Thermosynechococcus vestitus | NDP + sucrose | - |
r | |
TDP-glucose + D-fructose | lower activity in sucrose cleavage | Thermosynechococcus vestitus | TDP + sucrose | - |
r | |
UDP-glucose + D-fructose | lower activity in sucrose cleavage | Thermosynechococcus vestitus | UDP + sucrose | - |
r |
Subunits | Comment | Organism |
---|---|---|
homotetramer | 4 * 95000, recombinant enzyme, SDS-PAGE | Thermosynechococcus vestitus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
sucrose cleavage with UDP | Thermosynechococcus vestitus |
70 | - |
sucrose cleavage with ADP | Thermosynechococcus vestitus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 75 | activity range, profile overview | Thermosynechococcus vestitus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
the enzyme remains stable after 10 min incubation up to 50°C, but activity sharply decays beyond 55°C. Addition of 1 mM ADP, 10 mM UDP, or 200 mM Suc enhances the thermal stability by almost 5°C | Thermosynechococcus vestitus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
6 | - |
sucrose | pH 7.0, 37°C, purified recombinant enzyme, with ADP in sucrose cleavage | Thermosynechococcus vestitus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
sucrose cleavage with CDP, GDP, TDP, and UDP | Thermosynechococcus vestitus |
8 | - |
sucrose cleavage with ADP | Thermosynechococcus vestitus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 9 | sucrose cleavage, activities with CDP, GDP, TDP, and UDP only occur at pH 7.0-8.0 and are very low at pH 9.0, while the activity with ADP is highest at pH 8.0 and higher than 60% of maximal activity in the pH range of 7.0-9.0 | Thermosynechococcus vestitus |
General Information | Comment | Organism |
---|---|---|
metabolism | interaction between sucrose and glycogen metabolism | Thermosynechococcus vestitus |
physiological function | in filamentous cyanobacteria, the sucrose cleavage direction plays a key physiological function in carbon metabolism, nitrogen fixation, and stress tolerance | Thermosynechococcus vestitus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.38 | - |
D-fructose | pH 7.0, 37°C, recombinant enzyme, with UDP-glucose | Thermosynechococcus vestitus | |
0.406 | - |
D-fructose | pH 7.0, 37°C, recombinant enzyme, with ADP-glucose | Thermosynechococcus vestitus | |
2.673 | - |
UDP-glucose | pH 7.0, 37°C, recombinant enzyme | Thermosynechococcus vestitus | |
70.09 | - |
ADP-glucose | pH 7.0, 37°C, recombinant enzyme | Thermosynechococcus vestitus |