Inhibitors | Comment | Organism | Structure |
---|---|---|---|
ADP | 2 mM, less than 15% inhibition | Oryctolagus cuniculus | |
ATP | 2 mM, less than 15% inhibition | Oryctolagus cuniculus | |
deoxycholate | - |
Oryctolagus cuniculus | |
GDP | 0.02 mM, 84% inhibition; 2 mM, 84% inhibition | Oryctolagus cuniculus | |
GMP | 0.02 mM, 30% inhibition; 2 mM, 30% inhibition | Oryctolagus cuniculus | |
GTP | 0.02 mM, 40% inhibition; 2 mM, 40% inhibition | Oryctolagus cuniculus | |
additional information | less than 15% inhibition at 2 mM: ADP, ATP, UDP, UDP-glucose, UDP-galactose and UDP-N-acetylglucosamine; not inhibited by EDTA | Oryctolagus cuniculus | |
taurocholate | - |
Oryctolagus cuniculus | |
UDP | 2 mM, less than 15% inhibition | Oryctolagus cuniculus | |
UDP-galactose | 2 mM, less than 15% inhibition | Oryctolagus cuniculus | |
UDP-glucose | 2 mM, less than 15% inhibition | Oryctolagus cuniculus | |
UDP-N-acetylglucosamine | 2 mM, less than 15% inhibition | Oryctolagus cuniculus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00064 | - |
D-Man-alpha-(1->3)-(D-Man-alpha-(1->6))-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol | pH 7.0, 37°C | Oryctolagus cuniculus | |
0.00064 | - |
oligosaccharide-lipid acceptor | based on the assumption of incorporation of only a single mannosyl unit into the product | Oryctolagus cuniculus | |
0.0047 | - |
GDPmannose | - |
Oryctolagus cuniculus | |
0.0047 | - |
GDP-D-mannose | pH 7.0, 37°C | Oryctolagus cuniculus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
microsome | - |
Oryctolagus cuniculus | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | no requirement of divalent cations | Oryctolagus cuniculus | |
additional information | reaction is independent of the addition of divalent cations | Oryctolagus cuniculus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
GDPmannose + oligosaccharide-lipid acceptor | Oryctolagus cuniculus | biosynthesis of oligosaccharide-lipids | GDP + alpha-mannosyl-1,2-oligosaccharide-lipid | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Oryctolagus cuniculus | - |
- |
- |
Oryctolagus cuniculus | - |
New Zealand white rabbits | - |
Purification (Comment) | Organism |
---|---|
partial | Oryctolagus cuniculus |
solubilized with 0.15% v/v Nonidet P-40, partial | Oryctolagus cuniculus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Oryctolagus cuniculus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Oryctolagus cuniculus |
Storage Stability | Organism |
---|---|
0-4°C, solubilized enzyme, at least 2 weeks, stable | Oryctolagus cuniculus |
0°C, partially purified enzyme, 5 days, stable | Oryctolagus cuniculus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 GDP-alpha-D-mannose + D-Man-alpha-(1->3)-(D-Man-alpha-(1->6))-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol | - |
Oryctolagus cuniculus | 2 UDP + D-Man-alpha-(1->2)-D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-(D-Man-alpha-(1->6))-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol | - |
? | |
GDP-mannose + oligosaccharide-lipid acceptor | acceptor isolated from pig liver | Oryctolagus cuniculus | GDP + alpha-mannosyl-1,2-oligosaccharide-lipid | presumably a heptasaccharide with a 1,6-linked branched glycosyl unit with 65% of the newly added mannosyl units in a terminal nonreducing position, structure may be Manalpha(1-2)Man-Man(Man(1-6))Man-GlcNAc-GlcNAc | ? | |
GDPmannose + oligosaccharide-lipid acceptor | biosynthesis of oligosaccharide-lipids | Oryctolagus cuniculus | GDP + alpha-mannosyl-1,2-oligosaccharide-lipid | - |
? | |
additional information | no substrate: dolichol-phosphat | Oryctolagus cuniculus | additional information | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Oryctolagus cuniculus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | 7.5 | - |
Oryctolagus cuniculus |