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Literature summary for 2.4.1.132 extracted from
- The biosynthesis of oligosaccharide-lipids. Partial purification and characterization of mannosyltransferase II (1981), J. Biol. Chem., 256, 12899-12904.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| dolichol | stimulates enzyme in the presence of inhibitory concentrations of phosphatidylcholine | Oryctolagus cuniculus |  |
| dolichyl phosphate | stimulates enzyme in the presence of inhibitory concentrations of phosphatidylcholine | Oryctolagus cuniculus | |
| Nonidet P-40 | activation, up to 0.0225% v/v | Oryctolagus cuniculus | |
General Stability
| General Stability | Organism |
|---|---|
| glycerol, 0.5% v/v, stabilizes during storage and purification | Oryctolagus cuniculus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| ADP | weak | Oryctolagus cuniculus | |
| ATP | weak | Oryctolagus cuniculus | |
| EDTA | - |
Oryctolagus cuniculus | |
| GDP | 50% inhibition at 0.0006 mM | Oryctolagus cuniculus | |
| GDPglucose | - |
Oryctolagus cuniculus | |
| glycerol | 1-2% v/v | Oryctolagus cuniculus | |
| GMP | 50% inhibition at 0.094 mM | Oryctolagus cuniculus | |
| GTP | 50% inhibition at 0.006 mM | Oryctolagus cuniculus | |
| additional information | no inhibition by amphomycin | Oryctolagus cuniculus | |
| NiCl2 | - |
Oryctolagus cuniculus | |
| UDP | weak | Oryctolagus cuniculus | |
| UDP-N-acetylglucosamine | weak | Oryctolagus cuniculus | |
| UDPglucose | weak | Oryctolagus cuniculus | |
| UTP | weak | Oryctolagus cuniculus | |
| ZnCl2 | - |
Oryctolagus cuniculus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00021 | - |
tetrasaccharide-diphosphoryl-lipid | detergent-stimulated | Oryctolagus cuniculus | |
| 0.00065 | - |
GDPmannose | detergent-stimulated | Oryctolagus cuniculus | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| microsome | - |
Oryctolagus cuniculus | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | activation, about 60% as effective as Mg2+ | Oryctolagus cuniculus | |
| Co2+ | activation, about 4% as effective as Mg2+ | Oryctolagus cuniculus | |
| Mg2+ | requirement | Oryctolagus cuniculus | |
| Mg2+ | 10 mM | Oryctolagus cuniculus | |
| Mn2+ | activation, about 40% as effective as Mg2+ | Oryctolagus cuniculus | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| GDPmannose + tetrasaccharide-diphosphoryl-lipid | Oryctolagus cuniculus | involved in the biosynthesis of asparagine-linked saccharide chains of mammalian glycoproteins | GDP + mannosyl-alpha-1,3-tetrasaccharide-diphosphoryl-lipid | - |
? | |
| GDPmannose + tetrasaccharide-diphosphoryl-lipid | Oryctolagus cuniculus | the 1,3-linked mannosyl residue in mammalian lipid-linked oligosaccharide of the structure Glc3Man9GlcNAc2 is produced by this enzyme | GDP + mannosyl-alpha-1,3-tetrasaccharide-diphosphoryl-lipid | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Oryctolagus cuniculus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial | Oryctolagus cuniculus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| liver | - |
Oryctolagus cuniculus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
- |
Oryctolagus cuniculus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| GDPmannose + tetrasaccharide-diphosphoryl-lipid | no acceptor is dolichol-phosphate | Oryctolagus cuniculus | GDP + mannosyl-alpha-1,3-tetrasaccharide-diphosphoryl-lipid | - |
? | |
| GDPmannose + tetrasaccharide-diphosphoryl-lipid | involved in the biosynthesis of asparagine-linked saccharide chains of mammalian glycoproteins | Oryctolagus cuniculus | GDP + mannosyl-alpha-1,3-tetrasaccharide-diphosphoryl-lipid | - |
? | |
| GDPmannose + tetrasaccharide-diphosphoryl-lipid | the 1,3-linked mannosyl residue in mammalian lipid-linked oligosaccharide of the structure Glc3Man9GlcNAc2 is produced by this enzyme | Oryctolagus cuniculus | GDP + mannosyl-alpha-1,3-tetrasaccharide-diphosphoryl-lipid | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 37 | - |
assay at | Oryctolagus cuniculus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.8 | 7.3 | - |
Oryctolagus cuniculus |
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