Literature summary for 2.4.1.132 extracted from

K�mpf, M.; Absmanner, B.; Schwarz, M.; Lehle, L.
Biochemical characterization and membrane topology of Alg2 from Saccharomyces cerevisiae as a bifunctional alpha1,3- and 1,6-mannosyltransferase involved in lipid-linked oligosaccharide biosynthesis (2009), J. Biol. Chem., 284, 11900-11912.

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Protein Variants

Protein Variants	Comment	Organism
D203A	mutation has no influence on Alg2 function	Saccharomyces cerevisiae
D248A	mutation has no influence on Alg2 function	Saccharomyces cerevisiae
E264A	mutation has no influence on Alg2 function	Saccharomyces cerevisiae
E335A	mutant has some residual activity	Saccharomyces cerevisiae
E343A	inactive mutant enzyme	Saccharomyces cerevisiae
G337A	mutation has no influence on Alg2 function	Saccharomyces cerevisiae
G337E	nonfunctional enzyme variant	Saccharomyces cerevisiae
G337R	nonfunctional enzyme variant	Saccharomyces cerevisiae
G338A	mutation has no influence on Alg2 function	Saccharomyces cerevisiae
H336A	mutation has no influence on Alg2 function	Saccharomyces cerevisiae
K206A	mutation has no influence on Alg2 function	Saccharomyces cerevisiae
K210A	mutation has no influence on Alg2 function	Saccharomyces cerevisiae
K229A	mutation has no effect on growth and glycosylation	Saccharomyces cerevisiae
K230A	mutation causes loss of Alg2 activity	Saccharomyces cerevisiae
K251A	mutation has no influence on Alg2 function	Saccharomyces cerevisiae
additional information	mutational analysis of Alg2 and identification of amino acids required for its activity. None of the four domains (predicted as transmembrane-spanning helices) is essential for transferase activity because truncated Alg2 variants can exert their function as long as Alg2 is associated with the endaplasmic reticulum by either its N- or C-terminal hydrophobic regions	Saccharomyces cerevisiae
N231A	mutation has no effect on growth and glycosylation	Saccharomyces cerevisiae
N392A	mutation has no influence on Alg2 function	Saccharomyces cerevisiae
P192A	mutation has no influence on Alg2 function	Saccharomyces cerevisiae
P359A	mutation has no influence on Alg2 function	Saccharomyces cerevisiae

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
membrane	-	Saccharomyces cerevisiae	16020	-

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
GDP-alpha-D-mannose + D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol	Saccharomyces cerevisiae	in eukaryotes, biosynthesis of N-glycans starts with the assembly of the common core oligosaccharide precursor Glc3Man9 GlcNAc2-PP-Dol, the glycan moiety of which is subsequently transferred onto selected Asn-Xaa-(Ser/Thr) acceptor sites of the nascent polypeptide chain by the oligosaccharyl-transferase complex	GDP + D-Man-alpha-(1->3)-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-GlcNAc-diphosphodolichol	-	?

Organism

Organism	UniProt	Comment	Textmining
Saccharomyces cerevisiae	P43636	-	-

Posttranslational Modification

Posttranslational Modification	Comment	Organism
no glycoprotein	-	Saccharomyces cerevisiae

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
GDP-alpha-D-mannose + D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol	in eukaryotes, biosynthesis of N-glycans starts with the assembly of the common core oligosaccharide precursor Glc3Man9 GlcNAc2-PP-Dol, the glycan moiety of which is subsequently transferred onto selected Asn-Xaa-(Ser/Thr) acceptor sites of the nascent polypeptide chain by the oligosaccharyl-transferase complex	Saccharomyces cerevisiae	GDP + D-Man-alpha-(1->3)-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-GlcNAc-diphosphodolichol	-	?
GDP-alpha-D-mannose + D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol	Alg2 is able to catalyze both the addition of the alpa1,3- and alpha1,6-linked mannose residue to Man1GlcNAc2-PP-Dol, forming Man2GlcNAc2-PP-Dol (cf EC 2.4.1.132) and subsequently to Man3GlcNAc2-PP-Dol	Saccharomyces cerevisiae	GDP + D-Man-alpha-(1->3)-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-GlcNAc-diphosphodolichol	-	?

Synonyms

Synonyms	Comment	Organism
Alg2	-	Saccharomyces cerevisiae
Alg2 mannosyltransferase	-	Saccharomyces cerevisiae

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
26	-	assay at	Saccharomyces cerevisiae

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
6	-	assay at	Saccharomyces cerevisiae