Application | Comment | Organism |
---|---|---|
drug development | the enzyme is a target for the development of enzyme inhibitors as regulators of glycosaminoglycan synthesis in therapeutics | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Homo sapiens |
gene B4GALT7, recombinant expression of GST-tagged wild-type and mutant enzymes in CHOpgsB-618 cells, recombinant expression of the soluble GST-tagged mutant enzyme in Escherichia coli strain BL21(DE3) | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
D211N | inactive | Homo sapiens |
H195A | site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme | Homo sapiens |
H195A | the mutant shows reduced activity compared to the wild type enzyme | Homo sapiens |
H195Q | site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme | Homo sapiens |
H195Q | the mutant shows reduced activity compared to the wild type enzyme | Homo sapiens |
H195R | site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme | Homo sapiens |
H195R | the mutant shows reduced activity compared to the wild type enzyme | Homo sapiens |
additional information | construction of a soluble enzyme mutant by truncation of the first 60 N-terminal amino acids | Homo sapiens |
R226A | site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme | Homo sapiens |
R226A | the mutant shows reduced activity compared to the wild type enzyme | Homo sapiens |
R226K | site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme | Homo sapiens |
R226K | the mutant shows reduced activity compared to the wild type enzyme | Homo sapiens |
R270A | site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme | Homo sapiens |
R270A | the mutant shows reduced activity compared to the wild type enzyme | Homo sapiens |
R270K | site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme | Homo sapiens |
R270K | the mutant shows reduced activity compared to the wild type enzyme | Homo sapiens |
Y194A | inactive | Homo sapiens |
Y194A | site-directed mutagenesis, inactive mutant | Homo sapiens |
Y194F | inactive | Homo sapiens |
Y194F | site-directed mutagenesis, inactive mutant | Homo sapiens |
Y196A | inactive | Homo sapiens |
Y196A | site-directed mutagenesis, inactive mutant | Homo sapiens |
Y196F | site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme | Homo sapiens |
Y196F | the mutant shows reduced activity compared to the wild type enzyme | Homo sapiens |
Y199A | inactive | Homo sapiens |
Y199A | site-directed mutagenesis, inactive mutant | Homo sapiens |
Y199F | site-directed mutagenesis, the mutant shows reduced activity compared to the wild-type enzyme | Homo sapiens |
Y199F | the mutant shows reduced activity compared to the wild type enzyme | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
4-methylumbelliferyl 4-deoxy-beta-D-fluoroxylose | - |
Homo sapiens | |
4-methylumbelliferyl 4-deoxy-beta-D-xylopyranoside | - |
Homo sapiens | |
4-methylumbelliferyl 4-deoxy-beta-D-xyloside | - |
Homo sapiens | |
4-methylumbelliferyl 4-fluoro-beta-D-xylopyranoside | 50% inhibition at 2 mM. The strategic position of Tyr194 forming stacking interactions with the aglycone, and the hydrogen bond between the His195 nitrogen backbone and the carbonyl group of the coumarinyl molecule to develop a tight binder of the enzyme. This leads to the synthesis of 4-deoxy-4-fluoroxylose linked to 4-methylumbelliferone that inhibits enzyme activity in vitro with a Ki 10 times lower than the Km value and efficiently impairs glycosaminoglycan synthesis in a cell assay | Homo sapiens | |
additional information | not inhibited by naphthyl 4-deoxy-beta-D-xylopyranoside; structure-guided design of enzyme inhibitors, xyloside inhibitors design and in vitro and in cellulo competition assays, overview | Homo sapiens | |
naphthyl 4-deoxy-beta-D-xylopyranoside | slight inhibition | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Michaelis-Menten kinetics | Homo sapiens | |
0.22 | - |
UDP-alpha-D-galactose | pH 7.0, 37°C, recombinant wild-type enzyme | Homo sapiens | |
0.22 | - |
UDP-alpha-D-galactose | wild type enzyme, at pH 7.0 and 37°C | Homo sapiens | |
0.27 | - |
UDP-alpha-D-galactose | mutant enzyme R270A, at pH 7.0 and 37°C | Homo sapiens | |
0.27 | - |
UDP-alpha-D-galactose | pH 7.0, 37°C, recombinant mutant R270A | Homo sapiens | |
0.29 | - |
UDP-alpha-D-galactose | mutant enzyme R226K, at pH 7.0 and 37°C | Homo sapiens | |
0.29 | - |
UDP-alpha-D-galactose | pH 7.0, 37°C, recombinant mutant R226K | Homo sapiens | |
0.32 | - |
UDP-alpha-D-galactose | mutant enzyme H195R, at pH 7.0 and 37°C | Homo sapiens | |
0.32 | - |
UDP-alpha-D-galactose | mutant enzyme Y199F, at pH 7.0 and 37°C | Homo sapiens | |
0.32 | - |
UDP-alpha-D-galactose | pH 7.0, 37°C, recombinant mutant H195R | Homo sapiens | |
0.32 | - |
UDP-alpha-D-galactose | pH 7.0, 37°C, recombinant mutant Y199F | Homo sapiens | |
0.33 | - |
UDP-alpha-D-galactose | mutant enzyme H195Q, at pH 7.0 and 37°C | Homo sapiens | |
0.33 | - |
UDP-alpha-D-galactose | pH 7.0, 37°C, recombinant mutant H195Q | Homo sapiens | |
0.34 | - |
UDP-alpha-D-galactose | mutant enzyme R226A, at pH 7.0 and 37°C | Homo sapiens | |
0.34 | - |
UDP-alpha-D-galactose | mutant enzyme Y196F, at pH 7.0 and 37°C | Homo sapiens | |
0.34 | - |
UDP-alpha-D-galactose | pH 7.0, 37°C, recombinant mutant R226A | Homo sapiens | |
0.34 | - |
UDP-alpha-D-galactose | pH 7.0, 37°C, recombinant mutant Y196F | Homo sapiens | |
0.35 | - |
4-methylumbelliferyl-beta-D-xylopyranoside | pH 7.0, 37°C, recombinant wild-type enzyme | Homo sapiens | |
0.35 | - |
4-methylumbelliferyl alpha-D-xylopyranoside | wild type enzyme, at pH 7.0 and 37°C | Homo sapiens | |
0.35 | - |
4-methylumbelliferyl alpha-D-xylopyranoside | mutant enzyme H195R, at pH 7.0 and 37°C | Homo sapiens | |
0.35 | - |
4-methylumbelliferyl-beta-D-xylopyranoside | pH 7.0, 37°C, recombinant mutant H195R | Homo sapiens | |
0.37 | - |
UDP-alpha-D-galactose | mutant enzyme R270K, at pH 7.0 and 37°C | Homo sapiens | |
0.37 | - |
UDP-alpha-D-galactose | pH 7.0, 37°C, recombinant mutant R270K | Homo sapiens | |
0.4 | - |
UDP-alpha-D-galactose | mutant enzyme H195A, at pH 7.0 and 37°C | Homo sapiens | |
0.4 | - |
UDP-alpha-D-galactose | pH 7.0, 37°C, recombinant mutant H195A | Homo sapiens | |
0.44 | - |
4-methylumbelliferyl alpha-D-xylopyranoside | mutant enzyme R226A, at pH 7.0 and 37°C | Homo sapiens | |
0.44 | - |
4-methylumbelliferyl-beta-D-xylopyranoside | pH 7.0, 37°C, recombinant mutant R226A | Homo sapiens | |
0.46 | - |
4-methylumbelliferyl alpha-D-xylopyranoside | mutant enzyme R226K, at pH 7.0 and 37°C | Homo sapiens | |
0.46 | - |
4-methylumbelliferyl-beta-D-xylopyranoside | pH 7.0, 37°C, recombinant mutant R226K | Homo sapiens | |
0.54 | - |
4-methylumbelliferyl alpha-D-xylopyranoside | mutant enzyme R270K, at pH 7.0 and 37°C | Homo sapiens | |
0.54 | - |
4-methylumbelliferyl-beta-D-xylopyranoside | pH 7.0, 37°C, recombinant mutant R270K | Homo sapiens | |
0.55 | - |
4-methylumbelliferyl alpha-D-xylopyranoside | mutant enzyme H195Q, at pH 7.0 and 37°C | Homo sapiens | |
0.55 | - |
4-methylumbelliferyl-beta-D-xylopyranoside | pH 7.0, 37°C, recombinant mutant H195Q | Homo sapiens | |
0.59 | - |
4-methylumbelliferyl alpha-D-xylopyranoside | mutant enzyme Y199F, at pH 7.0 and 37°C | Homo sapiens | |
0.59 | - |
4-methylumbelliferyl-beta-D-xylopyranoside | pH 7.0, 37°C, recombinant mutant Y199F | Homo sapiens | |
0.6 | - |
4-methylumbelliferyl alpha-D-xylopyranoside | mutant enzyme R270A, at pH 7.0 and 37°C | Homo sapiens | |
0.6 | - |
4-methylumbelliferyl-beta-D-xylopyranoside | pH 7.0, 37°C, recombinant mutant R270A | Homo sapiens | |
0.64 | - |
4-methylumbelliferyl alpha-D-xylopyranoside | mutant enzyme H195A, at pH 7.0 and 37°C | Homo sapiens | |
0.64 | - |
4-methylumbelliferyl-beta-D-xylopyranoside | pH 7.0, 37°C, recombinant mutant H195A | Homo sapiens | |
1.06 | - |
4-methylumbelliferyl alpha-D-xylopyranoside | mutant enzyme Y196F, at pH 7.0 and 37°C | Homo sapiens | |
1.06 | - |
4-methylumbelliferyl-beta-D-xylopyranoside | pH 7.0, 37°C, recombinant mutant Y196F | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | required | Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
39000 | - |
x * 39000, SDS-PAGE | Homo sapiens |
39000 | - |
x * 39000, glycosylated recombinant enzyme, SDS-PAGE | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q9UBV7 | - |
- |
Homo sapiens | Q9UBV7 | gene B4GALT7 | - |
Purification (Comment) | Organism |
---|---|
glutathione Sepharose column chromatography | Homo sapiens |
recombinant soluble GST-tagged mutant enzyme from Escherichia coli strain BL21(DE3) | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-alpha-D-galactose + 4-methylumbelliferyl alpha-D-xylopyranoside | - |
Homo sapiens | UDP + 4-methylumbelliferyl 4-O-beta-D-galactopyranosyl-beta-D-xylopyranoside | - |
? | |
UDP-alpha-D-galactose + 4-methylumbelliferyl-beta-D-xylopyranoside | the strategic position of Tyr194 forming stacking interactions with the aglycone, and the hydrogen bond between the His195 nitrogen backbone and the carbonyl group of the coumarinyl molecule to develop a tight binder of the enzyme. This leads to the synthesis of 4-deoxy-4-fluoroxylose linked to 4-methylumbelliferone that inhibits enzyme activity in vitro with a Ki 10 times lower than the Km value and efficiently impairs glycosaminoglycan synthesis in a cell assay. Molecular modeling of the enzyme's active site in the presence of UDP-alpha-D-galactose and 4-methylumbelliferyl-beta-D-xylopyranoside using the crystal structure of Drosophila melanogaster enzyme dbeta4GalT7, PDB code 4M4K, overview | Homo sapiens | UDP + ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 39000, SDS-PAGE | Homo sapiens |
? | x * 39000, glycosylated recombinant enzyme, SDS-PAGE | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
beta1,4-galactosyltransferase 7 | - |
Homo sapiens |
beta4GalT7 | - |
Homo sapiens |
hbeta4GalT7 | - |
Homo sapiens |
xylosylprotein beta1,4-galactosyltransferase | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.5 | - |
UDP-alpha-D-galactose | mutant enzyme Y196F, at pH 7.0 and 37°C | Homo sapiens | |
0.5 | - |
UDP-alpha-D-galactose | pH 7.0, 37°C, recombinant mutant Y196F | Homo sapiens | |
0.5 | - |
4-methylumbelliferyl-beta-D-xylopyranoside | pH 7.0, 37°C, recombinant mutant Y196F | Homo sapiens | |
0.77 | - |
UDP-alpha-D-galactose | mutant enzyme R270A, at pH 7.0 and 37°C | Homo sapiens | |
0.77 | - |
UDP-alpha-D-galactose | pH 7.0, 37°C, recombinant mutant R270A | Homo sapiens | |
0.77 | - |
4-methylumbelliferyl-beta-D-xylopyranoside | pH 7.0, 37°C, recombinant mutant R270A | Homo sapiens | |
0.81 | - |
UDP-alpha-D-galactose | mutant enzyme R270K, at pH 7.0 and 37°C | Homo sapiens | |
0.81 | - |
UDP-alpha-D-galactose | pH 7.0, 37°C, recombinant mutant R270K | Homo sapiens | |
0.81 | - |
4-methylumbelliferyl-beta-D-xylopyranoside | pH 7.0, 37°C, recombinant mutant R270K | Homo sapiens | |
0.89 | - |
UDP-alpha-D-galactose | mutant enzyme R226A, at pH 7.0 and 37°C | Homo sapiens | |
0.89 | - |
UDP-alpha-D-galactose | pH 7.0, 37°C, recombinant mutant R226A | Homo sapiens | |
0.89 | - |
4-methylumbelliferyl-beta-D-xylopyranoside | pH 7.0, 37°C, recombinant mutant R226A | Homo sapiens | |
1.21 | - |
UDP-alpha-D-galactose | mutant enzyme Y199F, at pH 7.0 and 37°C | Homo sapiens | |
1.21 | - |
UDP-alpha-D-galactose | pH 7.0, 37°C, recombinant mutant Y199F | Homo sapiens | |
1.21 | - |
4-methylumbelliferyl-beta-D-xylopyranoside | pH 7.0, 37°C, recombinant mutant Y199F | Homo sapiens | |
1.35 | - |
UDP-alpha-D-galactose | mutant enzyme R226K, at pH 7.0 and 37°C | Homo sapiens | |
1.35 | - |
UDP-alpha-D-galactose | pH 7.0, 37°C, recombinant mutant R226K | Homo sapiens | |
1.35 | - |
4-methylumbelliferyl-beta-D-xylopyranoside | pH 7.0, 37°C, recombinant mutant R226K | Homo sapiens | |
1.49 | - |
UDP-alpha-D-galactose | mutant enzyme H195R, at pH 7.0 and 37°C | Homo sapiens | |
1.49 | - |
UDP-alpha-D-galactose | pH 7.0, 37°C, recombinant mutant H195R | Homo sapiens | |
1.49 | - |
4-methylumbelliferyl-beta-D-xylopyranoside | pH 7.0, 37°C, recombinant mutant H195R | Homo sapiens | |
1.5 | - |
UDP-alpha-D-galactose | pH 7.0, 37°C, recombinant wild-type enzyme | Homo sapiens | |
1.5 | - |
4-methylumbelliferyl-beta-D-xylopyranoside | pH 7.0, 37°C, recombinant wild-type enzyme | Homo sapiens | |
1.51 | - |
UDP-alpha-D-galactose | wild type enzyme, at pH 7.0 and 37°C | Homo sapiens | |
1.62 | - |
UDP-alpha-D-galactose | mutant enzyme H195Q, at pH 7.0 and 37°C | Homo sapiens | |
1.62 | - |
UDP-alpha-D-galactose | pH 7.0, 37°C, recombinant mutant H195Q | Homo sapiens | |
1.62 | - |
4-methylumbelliferyl-beta-D-xylopyranoside | pH 7.0, 37°C, recombinant mutant H195Q | Homo sapiens | |
1.93 | - |
UDP-alpha-D-galactose | mutant enzyme H195A, at pH 7.0 and 37°C | Homo sapiens | |
1.93 | - |
UDP-alpha-D-galactose | pH 7.0, 37°C, recombinant mutant H195A | Homo sapiens | |
1.93 | - |
4-methylumbelliferyl-beta-D-xylopyranoside | pH 7.0, 37°C, recombinant mutant H195A | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Homo sapiens |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.03 | - |
4-methylumbelliferyl 4-deoxy-beta-D-fluoroxylose | at pH 7.0 and 37°C | Homo sapiens | |
0.03 | - |
4-methylumbelliferyl 4-fluoro-beta-D-xylopyranoside | pH 7.0, 37°C, recombinant wild-type enzyme | Homo sapiens | |
0.53 | - |
4-methylumbelliferyl 4-deoxy-beta-D-xyloside | at pH 7.0 and 37°C | Homo sapiens | |
0.53 | - |
4-methylumbelliferyl 4-deoxy-beta-D-xylopyranoside | pH 7.0, 37°C, recombinant wild-type enzyme | Homo sapiens |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.06 | - |
at pH 7.0 and 37°C | Homo sapiens | 4-methylumbelliferyl 4-deoxy-beta-D-fluoroxylose | |
0.06 | - |
pH 7.0, 37°C, recombinant wild-type enzyme | Homo sapiens | 4-methylumbelliferyl 4-fluoro-beta-D-xylopyranoside | |
1.28 | - |
at pH 7.0 and 37°C | Homo sapiens | 4-methylumbelliferyl 4-deoxy-beta-D-xyloside | |
1.28 | - |
pH 7.0, 37°C, recombinant wild-type enzyme | Homo sapiens | 4-methylumbelliferyl 4-deoxy-beta-D-xylopyranoside |
General Information | Comment | Organism |
---|---|---|
additional information | mapping of the organization of the acceptor binding pockex in complex with 4-methylumbelliferone-xylopyranoside as prototype substrate by combining molecular modeling, in vitro mutagenesis, and kinetic measurements, and in cellulo analysis of glycosaminoglycan anabolism and decorin glycosylation. The organization is governed, on one side, by three tyrosine residues, Tyr194, Tyr196, and Tyr199, which create a hydrophobic environment and provide stacking interactions with both xylopyranoside and aglycone rings. On the opposite side, a hydrogen-bond network is established between the charged amino acids Asp228, Asp229, and Arg226, and the hydroxyl groups of xylose | Homo sapiens |
physiological function | human xylosylprotein beta1,4-galactosyltransferase catalyzes the transfer of the first Gal residue of the tetrasaccharide linkage from the activated sugar UDP-galactose onto Xyl residues attached to the proteoglycan core protein. Because all glycosaminoglycans share the same stem core tetrasaccharide, the enzyme is a central enzyme in glycosaminoglycan biosynthesis | Homo sapiens |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.47 | - |
4-methylumbelliferyl alpha-D-xylopyranoside | mutant enzyme Y196F, at pH 7.0 and 37°C | Homo sapiens | |
0.47 | - |
4-methylumbelliferyl-beta-D-xylopyranoside | pH 7.0, 37°C, recombinant mutant Y196F | Homo sapiens | |
1.2 | - |
4-methylumbelliferyl alpha-D-xylopyranoside | mutant enzyme R270A, at pH 7.0 and 37°C | Homo sapiens | |
1.2 | - |
4-methylumbelliferyl-beta-D-xylopyranoside | pH 7.0, 37°C, recombinant mutant R270A | Homo sapiens | |
1.42 | - |
4-methylumbelliferyl alpha-D-xylopyranoside | mutant enzyme R270K, at pH 7.0 and 37°C | Homo sapiens | |
1.42 | - |
4-methylumbelliferyl-beta-D-xylopyranoside | pH 7.0, 37°C, recombinant mutant R270K | Homo sapiens | |
1.47 | - |
UDP-alpha-D-galactose | mutant enzyme Y196F, at pH 7.0 and 37°C | Homo sapiens | |
1.47 | - |
UDP-alpha-D-galactose | pH 7.0, 37°C, recombinant mutant Y196F | Homo sapiens | |
1.87 | - |
4-methylumbelliferyl alpha-D-xylopyranoside | mutant enzyme R226A, at pH 7.0 and 37°C | Homo sapiens | |
1.87 | - |
4-methylumbelliferyl-beta-D-xylopyranoside | pH 7.0, 37°C, recombinant mutant R226A | Homo sapiens | |
1.88 | - |
4-methylumbelliferyl alpha-D-xylopyranoside | mutant enzyme Y199F, at pH 7.0 and 37°C | Homo sapiens | |
1.88 | - |
4-methylumbelliferyl-beta-D-xylopyranoside | pH 7.0, 37°C, recombinant mutant Y199F | Homo sapiens | |
2.32 | - |
UDP-alpha-D-galactose | mutant enzyme R270K, at pH 7.0 and 37°C | Homo sapiens | |
2.32 | - |
UDP-alpha-D-galactose | pH 7.0, 37°C, recombinant mutant R270K | Homo sapiens | |
2.85 | - |
UDP-alpha-D-galactose | mutant enzyme R226A, at pH 7.0 and 37°C | Homo sapiens | |
2.85 | - |
UDP-alpha-D-galactose | pH 7.0, 37°C, recombinant mutant R226A | Homo sapiens | |
2.92 | - |
4-methylumbelliferyl alpha-D-xylopyranoside | mutant enzyme R226K, at pH 7.0 and 37°C | Homo sapiens | |
2.92 | - |
4-methylumbelliferyl-beta-D-xylopyranoside | pH 7.0, 37°C, recombinant mutant R226K | Homo sapiens | |
2.95 | - |
4-methylumbelliferyl alpha-D-xylopyranoside | mutant enzyme H195Q, at pH 7.0 and 37°C | Homo sapiens | |
2.95 | - |
4-methylumbelliferyl-beta-D-xylopyranoside | pH 7.0, 37°C, recombinant mutant H195Q | Homo sapiens | |
3 | - |
4-methylumbelliferyl alpha-D-xylopyranoside | mutant enzyme H195A, at pH 7.0 and 37°C | Homo sapiens | |
3 | - |
4-methylumbelliferyl-beta-D-xylopyranoside | pH 7.0, 37°C, recombinant mutant H195A | Homo sapiens | |
3.07 | - |
UDP-alpha-D-galactose | mutant enzyme R270A, at pH 7.0 and 37°C | Homo sapiens | |
3.07 | - |
UDP-alpha-D-galactose | pH 7.0, 37°C, recombinant mutant R270A | Homo sapiens | |
4.03 | - |
4-methylumbelliferyl alpha-D-xylopyranoside | mutant enzyme H195R, at pH 7.0 and 37°C | Homo sapiens | |
4.03 | - |
4-methylumbelliferyl-beta-D-xylopyranoside | pH 7.0, 37°C, recombinant mutant H195R | Homo sapiens | |
4.05 | - |
UDP-alpha-D-galactose | mutant enzyme Y199F, at pH 7.0 and 37°C | Homo sapiens | |
4.05 | - |
UDP-alpha-D-galactose | pH 7.0, 37°C, recombinant mutant Y199F | Homo sapiens | |
4.17 | - |
4-methylumbelliferyl alpha-D-xylopyranoside | wild type enzyme, at pH 7.0 and 37°C | Homo sapiens | |
4.17 | - |
4-methylumbelliferyl-beta-D-xylopyranoside | pH 7.0, 37°C, recombinant wild-type enzyme | Homo sapiens | |
4.7 | - |
UDP-alpha-D-galactose | mutant enzyme R226K, at pH 7.0 and 37°C | Homo sapiens | |
4.7 | - |
UDP-alpha-D-galactose | pH 7.0, 37°C, recombinant mutant R226K | Homo sapiens | |
4.85 | - |
UDP-alpha-D-galactose | mutant enzyme H195A, at pH 7.0 and 37°C | Homo sapiens | |
4.85 | - |
UDP-alpha-D-galactose | mutant enzyme H195R, at pH 7.0 and 37°C | Homo sapiens | |
4.85 | - |
UDP-alpha-D-galactose | pH 7.0, 37°C, recombinant mutant H195A | Homo sapiens | |
4.92 | - |
UDP-alpha-D-galactose | pH 7.0, 37°C, recombinant mutant H195Q | Homo sapiens | |
4.92 | - |
UDP-alpha-D-galactose | pH 7.0, 37°C, recombinant mutant H195R | Homo sapiens | |
7.08 | - |
UDP-alpha-D-galactose | wild type enzyme, at pH 7.0 and 37°C | Homo sapiens | |
7.08 | - |
UDP-alpha-D-galactose | pH 7.0, 37°C, recombinant wild-type enzyme | Homo sapiens |