Activating Compound | Comment | Organism | Structure |
---|---|---|---|
D-fructose-1-phosphate | slight activation | Spinacia oleracea | |
D-glucose-1-phosphate | slight activation | Spinacia oleracea | |
glucose-6-phosphate | - |
Spinacia oleracea |
General Stability | Organism |
---|---|
20% ethylene glycol and 0.2 M KCl stabilize activity | Spinacia oleracea |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
diphosphate | slight inhibition | Spinacia oleracea | |
NaF | strong inhibition at 20 mM | Spinacia oleracea | |
phosphate | in absence of glucose-6-phosphate, no inhibition at pH 5.5, inhibitory at alkaline pH | Spinacia oleracea | |
phosphoenolpyruvate | slight inhibition | Spinacia oleracea | |
UDP | - |
Spinacia oleracea |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.7 | - |
D-fructose 6-phosphate | in presence of 5 mM glucose 6-phosphate | Spinacia oleracea | |
3.2 | - |
D-fructose 6-phosphate | in the absence of glucose 6-phosphate | Spinacia oleracea |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
460000 | - |
gel filtration | Spinacia oleracea |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-alpha-D-glucose + D-fructose 6-phosphate | Spinacia oleracea | catalyzes the penultimate step of sucrose synthesis | UDP + sucrose 6-phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Spinacia oleracea | - |
- |
- |
Purification (Comment) | Organism |
---|---|
gel filtration is carried out in presence of 20% ethylene glycol and 0.2 M KCl to stabilize activity | Spinacia oleracea |
partial | Spinacia oleracea |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Spinacia oleracea | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-alpha-D-glucose + D-fructose 6-phosphate | - |
Spinacia oleracea | UDP + sucrose 6-phosphate | - |
? | |
UDP-alpha-D-glucose + D-fructose 6-phosphate | catalyzes the penultimate step of sucrose synthesis | Spinacia oleracea | UDP + sucrose 6-phosphate | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
broad optimum | Spinacia oleracea |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5.5 | 9 | - |
Spinacia oleracea |