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Literature summary for 2.4.1.14 extracted from
- Purification and characterization of sucrose-phosphate synthase from Prosopis juliflora (1997), Phytochemistry, 46, 441-447.
No PubMed abstract available
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| glucose-6-phosphate | - |
Spinacia oleracea |  |
| glucose-6-phosphate | - |
Prosopis juliflora | |
| Mg2+ | stimulates enzyme activity | Spinacia oleracea | |
| Mg2+ | stimulates enzyme activity | Prosopis juliflora | |
| Mn2+ | stimulates enzyme activity | Prosopis juliflora | |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| ADP | no inhibition | Prosopis juliflora | |
| ADP | - |
Spinacia oleracea | |
| ATP | reversible by addition of Mg2+ | Prosopis juliflora | |
| ATP | - |
Spinacia oleracea | |
| CTP | reversible by addition of Mg2+ | Prosopis juliflora | |
| fluoride | strong inhibition | Prosopis juliflora | |
| fluoride | - |
Spinacia oleracea | |
| GTP | reversible by addition of Mg2+ | Prosopis juliflora | |
| ITP | reversible by addition of Mg2+ | Prosopis juliflora | |
| p-chloromercuribenzoate | activity can be restored by addition of DTT or 2-mercaptoethanol | Prosopis juliflora | |
| phosphate | competitive with UDP-glucose | Prosopis juliflora | |
| phosphate | competitive with UDP-glucose | Spinacia oleracea | |
| sulfate | - |
Prosopis juliflora | |
| sulfate | - |
Spinacia oleracea | |
| UDP | competitive with UDP-glucose | Prosopis juliflora | |
| UDP | - |
Spinacia oleracea | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2 | - |
D-fructose 6-phosphate | Km is not effected by addition of 20 mM glucose 6-phosphate or 15 mM phosphate | Prosopis juliflora | |
| 4.6 | - |
UDP-glucose | in presence of 20 mM glucose 6-phosphate | Prosopis juliflora | |
| 20 | - |
UDP-glucose | - |
Prosopis juliflora | |
| 77 | - |
UDP-glucose | in presence of 15 mM phosphate | Prosopis juliflora | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | stimulates enzyme activity | Spinacia oleracea | |
| Mg2+ | stimulates enzyme activity | Prosopis juliflora | |
| Mn2+ | - |
Spinacia oleracea | |
| Mn2+ | stimulates enzyme activity | Prosopis juliflora | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 443000 | - |
gel filtration | Prosopis juliflora |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| UDP-alpha-D-glucose + D-fructose 6-phosphate | Spinacia oleracea | catalyzes the penultimate step of sucrose synthesis | UDP + sucrose 6-phosphate | - |
? | |
| UDP-alpha-D-glucose + D-fructose 6-phosphate | Prosopis juliflora | catalyzes the penultimate step of sucrose synthesis | UDP + sucrose 6-phosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Prosopis juliflora | - |
- |
- |
| Spinacia oleracea | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| partial, at least 3 additional proteins could not be removed | Prosopis juliflora |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| leaf | - |
Spinacia oleracea | - |
| leaf | - |
Prosopis juliflora | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 28 | - |
purified enzyme, specific activity in crude extract is extremely low compared to spinach | Prosopis juliflora |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| UDP-alpha-D-glucose + D-fructose 6-phosphate | - |
Spinacia oleracea | UDP + sucrose 6-phosphate | - |
? | |
| UDP-alpha-D-glucose + D-fructose 6-phosphate | ordered bi-bi mechanism | Prosopis juliflora | UDP + sucrose 6-phosphate | - |
? | |
| UDP-alpha-D-glucose + D-fructose 6-phosphate | catalyzes the penultimate step of sucrose synthesis | Spinacia oleracea | UDP + sucrose 6-phosphate | - |
? | |
| UDP-alpha-D-glucose + D-fructose 6-phosphate | catalyzes the penultimate step of sucrose synthesis | Prosopis juliflora | UDP + sucrose 6-phosphate | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
- |
Prosopis juliflora |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
- |
Prosopis juliflora |
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