Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | recombinant enzyme expressed in Escherichia coli is not activated by glucose 6-phosphate | Synechocystis sp. |
Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli. The Synechocystis sps gene is introduced into tobacco, rice and tomato under the control of constitutive promoters. The Synechocystis SPS protein is expressed at high level. However SPS activities and carbon partitioning in leaves from transgenic and wild-type plants are not significantly different. The purified enzymes have full catalytic activity. It is proposed that some other protein in plant cells binds to the Synechocystis SPS resulting in inhibition of the enzyme | Synechocystis sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | the Synechocystis sps gene is introduced into tobacco, rice and tomato under the control of constitutive promoters. The Synechocystis SPS protein is expressed at high level. However SPS activities and carbon partitioning in leaves from transgenic and wild-type plants are not significantly different. The purified enzymes have full catalytic activity. It is proposed that some other protein in plant cells binds to the Synechocystis SPS resulting in inhibition of the enzyme | Synechocystis sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.2 | - |
D-fructose 6-phosphate | pH 8.0, cosubstrate: UDP-glucose, enzyme expressed in Escherichia coli | Synechocystis sp. | |
0.3 | - |
D-fructose 6-phosphate | pH 8.0, cosubstrate: UDP-glucose, enzyme expressed in Nicotiana tabacum | Synechocystis sp. | |
1.5 | - |
GDP-glucose | pH 8.0, enzyme expressed in Escherichia coli | Synechocystis sp. | |
1.8 | - |
UDP-glucose | pH 8.0, enzyme expressed in Nicotiana tabacum | Synechocystis sp. | |
2 | - |
ADP-glucose | pH 8.0, enzyme expressed in Nicotiana tabacum | Synechocystis sp. | |
2.2 | - |
UDP-glucose | pH 8.0, enzyme expressed in Escherichia coli | Synechocystis sp. | |
2.4 | - |
GDP-glucose | pH 8.0, enzyme expressed in Nicotiana tabacum | Synechocystis sp. | |
2.7 | - |
ADP-glucose | pH 8.0, enzyme expressed in Escherichia coli | Synechocystis sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
82000 | - |
x * 82000, SDS-PAGE | Synechocystis sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-alpha-D-glucose + D-fructose 6-phosphate | Synechocystis sp. | - |
UDP + sucrose 6-phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Synechocystis sp. | - |
PCC 6803 | - |
Purification (Comment) | Organism |
---|---|
transgenic SPS from tobacco and rice | Synechocystis sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ADP-glucose + D-fructose 6-phosphate | - |
Synechocystis sp. | ADP + sucrose 6-phosphate | - |
? | |
GDP-glucose + D-fructose 6-phosphate | - |
Synechocystis sp. | GDP + sucrose 6-phosphate | - |
? | |
UDP-alpha-D-glucose + D-fructose 6-phosphate | - |
Synechocystis sp. | UDP + sucrose 6-phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 82000, SDS-PAGE | Synechocystis sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | - |
recombinant enzyme expressed in Escherichia coli | Synechocystis sp. |