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Literature summary for 2.4.1.14 extracted from

  • Lunn, J.E.; Gillespie, V.J.; Furbank, R.T.
    Expression of a cyanobacterial sucrose-phosphate synthase from Synechocystis sp. PCC 6803 in transgenic plants (2003), J. Exp. Bot., 54, 223-237.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
additional information recombinant enzyme expressed in Escherichia coli is not activated by glucose 6-phosphate Synechocystis sp.

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli. The Synechocystis sps gene is introduced into tobacco, rice and tomato under the control of constitutive promoters. The Synechocystis SPS protein is expressed at high level. However SPS activities and carbon partitioning in leaves from transgenic and wild-type plants are not significantly different. The purified enzymes have full catalytic activity. It is proposed that some other protein in plant cells binds to the Synechocystis SPS resulting in inhibition of the enzyme Synechocystis sp.

Inhibitors

Inhibitors Comment Organism Structure
additional information the Synechocystis sps gene is introduced into tobacco, rice and tomato under the control of constitutive promoters. The Synechocystis SPS protein is expressed at high level. However SPS activities and carbon partitioning in leaves from transgenic and wild-type plants are not significantly different. The purified enzymes have full catalytic activity. It is proposed that some other protein in plant cells binds to the Synechocystis SPS resulting in inhibition of the enzyme Synechocystis sp.

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.2
-
D-fructose 6-phosphate pH 8.0, cosubstrate: UDP-glucose, enzyme expressed in Escherichia coli Synechocystis sp.
0.3
-
D-fructose 6-phosphate pH 8.0, cosubstrate: UDP-glucose, enzyme expressed in Nicotiana tabacum Synechocystis sp.
1.5
-
GDP-glucose pH 8.0, enzyme expressed in Escherichia coli Synechocystis sp.
1.8
-
UDP-glucose pH 8.0, enzyme expressed in Nicotiana tabacum Synechocystis sp.
2
-
ADP-glucose pH 8.0, enzyme expressed in Nicotiana tabacum Synechocystis sp.
2.2
-
UDP-glucose pH 8.0, enzyme expressed in Escherichia coli Synechocystis sp.
2.4
-
GDP-glucose pH 8.0, enzyme expressed in Nicotiana tabacum Synechocystis sp.
2.7
-
ADP-glucose pH 8.0, enzyme expressed in Escherichia coli Synechocystis sp.

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
82000
-
x * 82000, SDS-PAGE Synechocystis sp.

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
UDP-alpha-D-glucose + D-fructose 6-phosphate Synechocystis sp.
-
UDP + sucrose 6-phosphate
-
?

Organism

Organism UniProt Comment Textmining
Synechocystis sp.
-
PCC 6803
-

Purification (Commentary)

Purification (Comment) Organism
transgenic SPS from tobacco and rice Synechocystis sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ADP-glucose + D-fructose 6-phosphate
-
Synechocystis sp. ADP + sucrose 6-phosphate
-
?
GDP-glucose + D-fructose 6-phosphate
-
Synechocystis sp. GDP + sucrose 6-phosphate
-
?
UDP-alpha-D-glucose + D-fructose 6-phosphate
-
Synechocystis sp. UDP + sucrose 6-phosphate
-
?

Subunits

Subunits Comment Organism
? x * 82000, SDS-PAGE Synechocystis sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
recombinant enzyme expressed in Escherichia coli Synechocystis sp.