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Literature summary for 2.4.1.14 extracted from

  • Verma, A.K.; Upadhyay, S.K.; Verma, P.C.; Solomon, S.; Singh, S.B.
    Functional analysis of sucrose phosphate synthase (SPS) and sucrose synthase (SS) in sugarcane (Saccharum) cultivars (2011), Plant Biol., 13, 325-332.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene SPSII, semiquantitative PCR expression analysis Saccharum officinarum

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+ required Saccharum officinarum

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
UDP-alpha-D-glucose + D-fructose 6-phosphate Saccharum officinarum
-
UDP + sucrose 6-phosphate
-
?

Organism

Organism UniProt Comment Textmining
Saccharum officinarum
-
high sucrose accumulating cultivars CoS96268 and CoS95255, and late maturing, low sucrose accumulating cultivars CoS97264, CoSe92423
-

Source Tissue

Source Tissue Comment Organism Textmining
internode SPS activity and transcript expression is higher in mature internodes compared with immature internodes in all the studied cultivars Saccharum officinarum
-
leaf
-
Saccharum officinarum
-
additional information high sugar cultivars showed increased transcript expression and enzyme activity of SPS compared to low sugar cultivars at all developmental stages Saccharum officinarum
-
stem
-
Saccharum officinarum
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDP-alpha-D-glucose + D-fructose 6-phosphate
-
Saccharum officinarum UDP + sucrose 6-phosphate
-
?

Synonyms

Synonyms Comment Organism
SPS
-
Saccharum officinarum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
assay at Saccharum officinarum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.4
-
assay at Saccharum officinarum

Expression

Organism Comment Expression
Saccharum officinarum SPS activity is positively correlated with sucrose and negatively correlated with hexose sugars down
Saccharum officinarum sucrose induces the enzyme, high sugar cultivars show increased transcript expression and enzyme activity of SPS compared to low sugar cultivars at all developmental stages. SPS activity is positively correlated with sucrose and negatively correlated with hexose sugars up

General Information

General Information Comment Organism
metabolism sucrose phosphate synthase and sucrose synthase, EC 2.4.1.13, are key enzymes in the synthesis and breakdown of sucrose in sugarcane Saccharum officinarum