Activating Compound | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Methylobacillus flagellatus | |
D-fructose 1,6-bisphosphate | - |
Methylobacillus flagellatus |
Cloned (Comment) | Organism |
---|---|
gene sps, sequence comparisons, phylogenetic analysis, recombinant expression of His6-tagged enzyme in Escherichia coli | Methylobacillus flagellatus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
AMP | - |
Methylobacillus flagellatus | |
D-Fructose 1-phosphate | slight inhibition | Methylobacillus flagellatus | |
D-glucose 6-phosphate | - |
Methylobacillus flagellatus | |
D-ribose 5-phosphate | - |
Methylobacillus flagellatus | |
additional information | poor effect by fructose and sucrose | Methylobacillus flagellatus | |
phosphate | slight inhibition | Methylobacillus flagellatus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.19 | - |
sucrose 6F-phosphate | pH 6.5, 45°C, recombinant His6-tagged enzyme | Methylobacillus flagellatus | |
1.2 | - |
D-fructose 6-phosphate | pH 6.5, 45°C, recombinant His6-tagged enzyme | Methylobacillus flagellatus | |
1.3 | - |
UDP-glucose | pH 6.5, 45°C, recombinant His6-tagged enzyme, in presence of fructose 1,6-bisphosphate | Methylobacillus flagellatus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | dependent on, optimal at 17.5 mM | Methylobacillus flagellatus | |
NaCl | activates, best at 2% | Methylobacillus flagellatus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
84132 | - |
4 * 84132, sequence calculation | Methylobacillus flagellatus |
336000 | - |
gel filtration | Methylobacillus flagellatus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Methylobacillus flagellatus | a bifunctional sucrose phosphate synthase/phosphatase (SPS/SPP) | ? | - |
? | |
UDP-glucose + D-fructose 6-phosphate | Methylobacillus flagellatus | - |
UDP + sucrose 6F-phosphate | - |
r |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methylobacillus flagellatus | Q1GY13 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant His6-tagged enzyme from Escherichia coli by nickel affinity chromatography to homogeneity | Methylobacillus flagellatus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | a bifunctional sucrose phosphate synthase/phosphatase (SPS/SPP) | Methylobacillus flagellatus | ? | - |
? | |
UDP + sucrose 6F-phosphate | - |
Methylobacillus flagellatus | UDP-glucose + D-fructose 6-phosphate | - |
r | |
UDP-glucose + D-fructose 6-phosphate | - |
Methylobacillus flagellatus | UDP + sucrose 6F-phosphate | - |
r |
Subunits | Comment | Organism |
---|---|---|
homotetramer | 4 * 84132, sequence calculation | Methylobacillus flagellatus |
Synonyms | Comment | Organism |
---|---|---|
SPS | - |
Methylobacillus flagellatus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
- |
Methylobacillus flagellatus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
- |
Methylobacillus flagellatus |