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Literature summary for 2.4.1.141 extracted from

  • Noffz, C.; Keppler-Ross, S.; Dean, N.
    Hetero-oligomeric interactions between early glycosyltransferases of the dolichol cycle (2009), Glycobiology, 19, 472-478.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.4.1.141

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Saccharomyces cerevisiae strain XGY151 Mesocricetus auratus

Localization

Localization Comment Organism GeneOntology No. Textmining
endoplasmic reticulum localized on the cytosolic face of the endoplasmic reticulum Mesocricetus auratus 5783
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
22600
-
 1 * 22600 + 1 * 27000, predicted molecular weights of subunits Alg13p and Alg14p, Alg13p is the soluble, cytosolic subunit that contains the catalytic domain, Alg14p is the membrane-spanning subunit that is required to recruit Alg13p to the cytosolic face of the endoplasmic reticulum Mesocricetus auratus
23000
-
 1 * 23000 + 1 * 30000, molecular weights of subunits Alg13p and Alg14p, gel filtration Mesocricetus auratus
27000
-
 1 * 22600 + 1 * 27000, predicted molecular weights of subunits Alg13p and Alg14p, Alg13p is the soluble, cytosolic subunit that contains the catalytic domain, Alg14p is the membrane-spanning subunit that is required to recruit Alg13p to the cytosolic face of the endoplasmic reticulum Mesocricetus auratus
30000
-
 1 * 23000 + 1 * 30000, molecular weights of subunits Alg13p and Alg14p, gel filtration Mesocricetus auratus

Organic Solvent Stability

Organic Solvent Comment Organism
Triton X-100 the Alg13p/Alg14p complex remains stable in the presence of 0.1% Triton X-100 Mesocricetus auratus

Organism

Organism UniProt Comment Textmining
Mesocricetus auratus
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDP-N-acetyl-alpha-D-glucosamine + N-acetyl-alpha-D-glucosaminyl-diphosphodolichol
-
 Mesocricetus auratus UDP + N,N'-diacetylchitobiosyl-diphosphodolichol
-
 ?

Subunits

Subunits Comment Organism
heterodimer 1 * 22600 + 1 * 27000, predicted molecular weights of subunits Alg13p and Alg14p, Alg13p is the soluble, cytosolic subunit that contains the catalytic domain, Alg14p is the membrane-spanning subunit that is required to recruit Alg13p to the cytosolic face of the endoplasmic reticulum Mesocricetus auratus
heterodimer 1 * 23000 + 1 * 30000, molecular weights of subunits Alg13p and Alg14p, gel filtration Mesocricetus auratus

Synonyms

Synonyms Comment Organism
Alg13p/Alg14p
-
 Mesocricetus auratus
UDP-GlcNAc transferase
-
 Mesocricetus auratus

General Information

General Information Comment Organism
physiological function the Alg7p and Alg13p/Alg14p UDP-GlcNAc transferases together mediate the earliest steps of the N-linked glycosylation pathway Mesocricetus auratus