Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.4.1.144 extracted from

  • Gu, J.; Isaji, T.; Sato, Y.; Kariya, Y.; Fukuda, T.
    Importance of N-glycosylation on alpha5beta1 integrin for its biological functions (2009), Biol. Pharm. Bull., 32, 780-785.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
highly metastatic melanoma B16 cells transfected with GnT-III and then injected into syngeneic mice via the tail vein Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDP-D-glucose + N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,6)-beta-D-mannosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
-
Homo sapiens UDP + N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3-(N-acetyl-beta-D-glucosaminyl-1,2-alpha-D-mannosyl-1,6)-(beta-D-glucosyl-1,4)-beta-D-mannosyl-1,4-N-acetyl-beta-D-glucosaminyl-R
-
?

Synonyms

Synonyms Comment Organism
GnT-III
-
Homo sapiens
N-acetylglucosaminyltransferase III
-
Homo sapiens

Expression

Organism Comment Expression
Homo sapiens in MDA-MB231 cells, an E-cadherin-deficient cell line, GnT-III expression is undetectable down
Homo sapiens E-cadherin-mediated cell-cell interaction upregulates GnT-III expression. Significant upregulation of GnT-III expression in epithelial cells that express basal levels of E-cadherin and GnT-III up

General Information

General Information Comment Organism
physiological function overexpression of GnT-III downregulates alpha5beta1 integrin-mediated cell spreading and migration, and the phosphorylation of the focal adhesion kinase. Overexpression of GnT-III slows E-cadherin turnover, resulting in increased E-cadherin expression on the surface of B16 melanoma cells. GnT-III inhibits GnT-V-induced cell migration. Overexpression of GnT-III inhibits cancer metastasis by at least two mechanisms: an enhancement in cell-cell adhesion and a downregulation of cell-ECM adhesion Homo sapiens