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Literature summary for 2.4.1.145 extracted from

  • Gleeson, P.A.; Schachter, H.
    Control of glycoprotein synthesis (1983), J. Biol. Chem., 258, 6162-6173.
    View publication on PubMed
Search for more literature for 2.4.1.145

Activating Compound

Activating Compound Comment Organism Structure
Triton X-100 maximum activation at 0.125% Gallus gallus

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
 Gallus gallus 16020
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
UDP-N-acetyl-D-glucosamine + 3-(2-[N-acetyl-beta-D-glucosaminyl]-alpha-D-mannosyl)-beta-D-mannosyl-R Gallus gallus
-
 UDP + 3-(2,4-bis[N-acetyl-beta-D-glucosaminyl]-alpha-D-mannosyl)-beta-D-mannosyl-R
-
 ?

Organism

Organism UniProt Comment Textmining
Gallus gallus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial Gallus gallus

Source Tissue

Source Tissue Comment Organism Textmining
oviduct
-
 Gallus gallus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 activities for different glycopeptides as acceptors Gallus gallus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDP-N-acetyl-D-glucosamine + 3-(2-[N-acetyl-beta-D-glucosaminyl]-alpha-D-mannosyl)-beta-D-mannosyl-R
-
 Gallus gallus UDP + 3-(2,4-bis[N-acetyl-beta-D-glucosaminyl]-alpha-D-mannosyl)-beta-D-mannosyl-R
-
 ?
UDP-N-acetyl-D-glucosamine + 3-(2-[N-acetyl-beta-D-glucosaminyl]-alpha-D-mannosyl)-beta-D-mannosyl-R maximal activity requires the presence of both terminal beta-1,2-linked N-acetylglucosamine residues in the substrate Gallus gallus UDP + 3-(2,4-bis[N-acetyl-beta-D-glucosaminyl]-alpha-D-mannosyl)-beta-D-mannosyl-R
-
 ?
UDP-N-acetyl-D-glucosamine + 3-(2-[N-acetyl-beta-D-glucosaminyl]-alpha-D-mannosyl)-beta-D-mannosyl-R R represents the remainder of the N-oligosaccharide in the glycoprotein acceptor, the enzyme adds N-cetylglucosamine in beta-1,4-linkage to the alpha-1,3-linked mannosyl residues of the trimannosyl core of N-glycosyloligosaccharides Gallus gallus UDP + 3-(2,4-bis[N-acetyl-beta-D-glucosaminyl]-alpha-D-mannosyl)-beta-D-mannosyl-R
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Gallus gallus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 in presence of 0.625% Triton X-100 Gallus gallus

pH Range

pH Minimum pH Maximum Comment Organism
6.5 7.5 depends on Triton X-100 concentration Gallus gallus