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Literature summary for 2.4.1.146 extracted from
- High-performance liquid chromatography assays for N-acetylglucosaminyltransferases involved in N- and O-glycan synthesis (1989), Methods Enzymol., 179, 351-397.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| Triton X-100 | stimulation, 0.1% v/v | Sus scrofa |  |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| EDTA | 83% inhibition at 10 mM | Sus scrofa | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.9 | - |
beta-D-galactosyl-1,3-(N-acetyl-D-glucosaminyl-1,6)-N-acetyl-D-galactosaminyl-o-nitrophenyl | - |
Sus scrofa | |
| 1.2 | - |
beta-D-galactosyl-1,3-(N-acetyl-D-glucosaminyl-1,6)-N-acetyl-D-galactosaminyl-benzyl | - |
Sus scrofa | |
| 1.6 | - |
UDP-N-acetyl-D-glucosamine | - |
Sus scrofa | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| membrane | - |
Sus scrofa | 16020 | - |
| microsome | - |
Sus scrofa | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | activation | Sus scrofa | |
| Mn2+ | activation, 10-40 mM | Sus scrofa | |
| additional information | no activation by Ca2+, Mg2+, Zn2+ | Sus scrofa |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| UDP-N-acetyl-D-glucosamine + beta-D-galactosyl-1,3-(N-acetyl-D-glucosaminyl-1,6)-N-acetyl-D-galactosaminyl-R | Rattus norvegicus | involved in elongation of O-glycan cores during mucin biosynthesis | UDP + N-acetyl-beta-D-glucosaminyl-1,3-beta-D-galactosyl-1,3-(N-acetyl-D-glucosaminyl-1,6)-N-acetyl-D-galactosaminyl-R | - |
? | |
| UDP-N-acetyl-D-glucosamine + beta-D-galactosyl-1,3-(N-acetyl-D-glucosaminyl-1,6)-N-acetyl-D-galactosaminyl-R | Sus scrofa | involved in elongation of O-glycan cores during mucin biosynthesis | UDP + N-acetyl-beta-D-glucosaminyl-1,3-beta-D-galactosyl-1,3-(N-acetyl-D-glucosaminyl-1,6)-N-acetyl-D-galactosaminyl-R | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| no activity in Canis familiaris | - |
submaxillary gland | - |
| Rattus norvegicus | - |
- |
- |
| Sus scrofa | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| colon | - |
Rattus norvegicus | - |
| colon | - |
Sus scrofa | - |
| gastric mucosa | - |
Sus scrofa | - |
| additional information | low activity or absent in rat liver, stomach and submaxillary glands and pig submaxillary glands | Rattus norvegicus | - |
| additional information | low activity or absent in rat liver, stomach and submaxillary glands and pig submaxillary glands | Sus scrofa | - |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -70°C, microsomal pig stomach enzyme preparation, detergent-free 0.25 M sucrose suspension, several years | Sus scrofa |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| UDP-N-acetyl-D-glucosamine + antifreeze glycoprotein | - |
Rattus norvegicus | UDP + N-acetyl-beta-D-glucosaminyl-1,3-antifreeze glycoprotein | - |
? | |
| UDP-N-acetyl-D-glucosamine + antifreeze glycoprotein | - |
Sus scrofa | UDP + N-acetyl-beta-D-glucosaminyl-1,3-antifreeze glycoprotein | - |
? | |
| UDP-N-acetyl-D-glucosamine + beta-D-galactosyl-1,3-(N-acetyl-D-glucosaminyl-1,6)-N-acetyl-D-galactosaminyl-benzyl | - |
Rattus norvegicus | UDP + N-acetyl-beta-D-glucosaminyl-1,3-beta-D-galactosyl-1,3-(N-acetyl-D-glucosaminyl-1,6)-N-acetyl-D-galactosaminyl-benzyl | - |
? | |
| UDP-N-acetyl-D-glucosamine + beta-D-galactosyl-1,3-(N-acetyl-D-glucosaminyl-1,6)-N-acetyl-D-galactosaminyl-benzyl | - |
Sus scrofa | UDP + N-acetyl-beta-D-glucosaminyl-1,3-beta-D-galactosyl-1,3-(N-acetyl-D-glucosaminyl-1,6)-N-acetyl-D-galactosaminyl-benzyl | - |
? | |
| UDP-N-acetyl-D-glucosamine + beta-D-galactosyl-1,3-(N-acetyl-D-glucosaminyl-1,6)-N-acetyl-D-galactosaminyl-o-nitrophenyl | - |
Rattus norvegicus | UDP + N-acetyl-beta-D-glucosaminyl-1,3-beta-D-galactosyl-1,3-(N-acetyl-D-glucosaminyl-1,6)-N-acetyl-D-galactosaminyl-o-nitrophenyl | - |
? | |
| UDP-N-acetyl-D-glucosamine + beta-D-galactosyl-1,3-(N-acetyl-D-glucosaminyl-1,6)-N-acetyl-D-galactosaminyl-o-nitrophenyl | - |
Sus scrofa | UDP + N-acetyl-beta-D-glucosaminyl-1,3-beta-D-galactosyl-1,3-(N-acetyl-D-glucosaminyl-1,6)-N-acetyl-D-galactosaminyl-o-nitrophenyl | - |
? | |
| UDP-N-acetyl-D-glucosamine + beta-D-galactosyl-1,3-(N-acetyl-D-glucosaminyl-1,6)-N-acetyl-D-galactosaminyl-p-nitrophenyl | - |
Rattus norvegicus | UDP + N-acetyl-beta-D-glucosaminyl-1,3-beta-D-galactosyl-1,3-(N-acetyl-D-glucosaminyl-1,6)-N-acetyl-D-galactosaminyl-p-nitrophenyl | - |
? | |
| UDP-N-acetyl-D-glucosamine + beta-D-galactosyl-1,3-(N-acetyl-D-glucosaminyl-1,6)-N-acetyl-D-galactosaminyl-p-nitrophenyl | - |
Sus scrofa | UDP + N-acetyl-beta-D-glucosaminyl-1,3-beta-D-galactosyl-1,3-(N-acetyl-D-glucosaminyl-1,6)-N-acetyl-D-galactosaminyl-p-nitrophenyl | - |
? | |
| UDP-N-acetyl-D-glucosamine + beta-D-galactosyl-1,3-(N-acetyl-D-glucosaminyl-1,6)-N-acetyl-D-galactosaminyl-R | substrate specificity | Rattus norvegicus | UDP + N-acetyl-beta-D-glucosaminyl-1,3-beta-D-galactosyl-1,3-(N-acetyl-D-glucosaminyl-1,6)-N-acetyl-D-galactosaminyl-R | - |
? | |
| UDP-N-acetyl-D-glucosamine + beta-D-galactosyl-1,3-(N-acetyl-D-glucosaminyl-1,6)-N-acetyl-D-galactosaminyl-R | substrate specificity | Sus scrofa | UDP + N-acetyl-beta-D-glucosaminyl-1,3-beta-D-galactosyl-1,3-(N-acetyl-D-glucosaminyl-1,6)-N-acetyl-D-galactosaminyl-R | - |
? | |
| UDP-N-acetyl-D-glucosamine + beta-D-galactosyl-1,3-(N-acetyl-D-glucosaminyl-1,6)-N-acetyl-D-galactosaminyl-R | i.e. core class 2 | Rattus norvegicus | UDP + N-acetyl-beta-D-glucosaminyl-1,3-beta-D-galactosyl-1,3-(N-acetyl-D-glucosaminyl-1,6)-N-acetyl-D-galactosaminyl-R | - |
? | |
| UDP-N-acetyl-D-glucosamine + beta-D-galactosyl-1,3-(N-acetyl-D-glucosaminyl-1,6)-N-acetyl-D-galactosaminyl-R | i.e. core class 2 | Sus scrofa | UDP + N-acetyl-beta-D-glucosaminyl-1,3-beta-D-galactosyl-1,3-(N-acetyl-D-glucosaminyl-1,6)-N-acetyl-D-galactosaminyl-R | - |
? | |
| UDP-N-acetyl-D-glucosamine + beta-D-galactosyl-1,3-(N-acetyl-D-glucosaminyl-1,6)-N-acetyl-D-galactosaminyl-R | R. not H | Rattus norvegicus | UDP + N-acetyl-beta-D-glucosaminyl-1,3-beta-D-galactosyl-1,3-(N-acetyl-D-glucosaminyl-1,6)-N-acetyl-D-galactosaminyl-R | - |
? | |
| UDP-N-acetyl-D-glucosamine + beta-D-galactosyl-1,3-(N-acetyl-D-glucosaminyl-1,6)-N-acetyl-D-galactosaminyl-R | R. not H | Sus scrofa | UDP + N-acetyl-beta-D-glucosaminyl-1,3-beta-D-galactosyl-1,3-(N-acetyl-D-glucosaminyl-1,6)-N-acetyl-D-galactosaminyl-R | - |
? | |
| UDP-N-acetyl-D-glucosamine + beta-D-galactosyl-1,3-(N-acetyl-D-glucosaminyl-1,6)-N-acetyl-D-galactosaminyl-R | polypeptide from mucin or antifreeze glycoprotein | Rattus norvegicus | UDP + N-acetyl-beta-D-glucosaminyl-1,3-beta-D-galactosyl-1,3-(N-acetyl-D-glucosaminyl-1,6)-N-acetyl-D-galactosaminyl-R | - |
? | |
| UDP-N-acetyl-D-glucosamine + beta-D-galactosyl-1,3-(N-acetyl-D-glucosaminyl-1,6)-N-acetyl-D-galactosaminyl-R | polypeptide from mucin or antifreeze glycoprotein | Sus scrofa | UDP + N-acetyl-beta-D-glucosaminyl-1,3-beta-D-galactosyl-1,3-(N-acetyl-D-glucosaminyl-1,6)-N-acetyl-D-galactosaminyl-R | - |
? | |
| UDP-N-acetyl-D-glucosamine + beta-D-galactosyl-1,3-(N-acetyl-D-glucosaminyl-1,6)-N-acetyl-D-galactosaminyl-R | involved in elongation of O-glycan cores during mucin biosynthesis | Rattus norvegicus | UDP + N-acetyl-beta-D-glucosaminyl-1,3-beta-D-galactosyl-1,3-(N-acetyl-D-glucosaminyl-1,6)-N-acetyl-D-galactosaminyl-R | - |
? | |
| UDP-N-acetyl-D-glucosamine + beta-D-galactosyl-1,3-(N-acetyl-D-glucosaminyl-1,6)-N-acetyl-D-galactosaminyl-R | involved in elongation of O-glycan cores during mucin biosynthesis | Sus scrofa | UDP + N-acetyl-beta-D-glucosaminyl-1,3-beta-D-galactosyl-1,3-(N-acetyl-D-glucosaminyl-1,6)-N-acetyl-D-galactosaminyl-R | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7 | - |
sharp optimum | Sus scrofa |
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