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Literature summary for 2.4.1.148 extracted from

  • Singh, J.; Khan, G.A.; Kinarsky, L.; Cheng, H.; Wilken, J.; Choi, K.H.; Bedows, E.; Sherman, S.; Cheng, P.W.
    Identification of disulfide bonds among the nine core 2 N-acetylglucosaminyltransferase-M cysteines conserved in the mucin beta6-N-acetylglucosaminyltransferase family (2004), J. Biol. Chem., 279, 38969-38977.
    View publication on PubMed
Search for more literature for 2.4.1.148

Cloned(Commentary)

Cloned (Comment) Organism
stable expression of the enzyme, lacking the cytoplasmic tail and the transmembrane domain, in CHO cells, secretion of the fully active recombinant enzyme to the culture medium Bos taurus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
52479
-
 x * 58000, recombinant enzyme, SDS-PAGE, x * 52479, sequence calculation Bos taurus
58000
-
 x * 58000, recombinant enzyme, SDS-PAGE, x * 52479, sequence calculation Bos taurus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Bos taurus the enzyme catalyzes the formation of all mucin beta1,6-N-acetylglucosaminides, including core 2, core 4, and blood group I structures ?
-
 ?

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein enzyme is N-glycosylated at one or two residues N72 and/or N108 Bos taurus

Purification (Commentary)

Purification (Comment) Organism
recombinant enzyme from CHO cell culture medium by nickel affinity chromatography Bos taurus

Reaction

Reaction Comment Organism Reaction ID
UDP-N-acetyl-D-glucosamine + N-acetyl-beta-D-glucosaminyl-(1->3)-N-acetyl-D-galactosaminyl-R = UDP + N-acetyl-beta-D-glucosaminyl-(1->6)-[N-acetyl-beta-D-glucosaminyl-(1->3)]-N-acetyl-D-galactosaminyl-R the cystine pair pattern influences the substrate specificity of the enzyme, comparison to the enzyme from mouse Bos taurus
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the enzyme catalyzes the formation of all mucin beta1,6-N-acetylglucosaminides, including core 2, core 4, and blood group I structures Bos taurus ?
-
 ?
additional information substrate specificities of wild-type and mutant enzyme, overview Bos taurus ?
-
 ?
UDP-N-acetyl-D-glucosamine + core 1 oligosaccharide
-
 Bos taurus UDP + core 2 oligosaccharide
-
 ?
UDP-N-acetyl-D-glucosamine + core 3 oligosaccharide
-
 Bos taurus UDP + core 4 oligosaccharide
-
 ?
UDP-N-acetyl-D-glucosamine + N-acetyl-beta-D-glucosaminyl-1,3-N-acetyl-D-galactosaminyl-R
-
 Bos taurus UDP + N-acetyl-beta-D-glucosaminyl-1,6-(N-acetyl-beta-D-glucosaminyl-1,3)-N-acetyl-D-galactosaminyl-R
-
 ?

Subunits

Subunits Comment Organism
? x * 58000, recombinant enzyme, SDS-PAGE, x * 52479, sequence calculation Bos taurus
More the enzyme possesses 9 cysteine residues, 8 of which forming disulfide bonds, Cys113 remains free, determination in the recombinant enzyme, overview Bos taurus

Synonyms

Synonyms Comment Organism
bC2GnT-M
-
 Bos taurus
core 2 N-acetylglucosaminyltransferase-M
-
 Bos taurus
More the enzyme belongs to the mucin beta6-N-acetylglucosaminyltransferase family Bos taurus