Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.4.1.149 extracted from

  • Fukuzumi, M.; Maruyama, S.; Sano, M.; Fukui, S.
    Comparison of the expression of cell surface poly-N-acetyllactosamine-type oligosaccharides in PC12 cells with those in its variant PC12D (2001), Glycobiology, 11, 481-494.
    View publication on PubMed
Search for more literature for 2.4.1.149

Inhibitors

Inhibitors Comment Organism Structure
additional information nerve growth factor stimulated PC-12 cells with reduced GnT-i activity compared to unstimulated cells, no effect in PC-12D cells Rattus norvegicus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.44
-
 Galbeta(1-4)GlcNAcbeta(1-4)GlcNAc-2-aminopyridine PC-12 cells Rattus norvegicus
0.9
-
 Galbeta(1-4)GlcNAcbeta(1-4)GlcNAc-2-aminopyridine PC-12D cells Rattus norvegicus
8
-
 UDP-N-acetyl-D-glucosamine PC-12 and PC-12D cells Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
UDP-N-acetyl-D-glucosamine + poly-N-acetyllactosamine Rattus norvegicus biosynthesis of poly-N-acetyllactosamine chains on N-linked oligosaccharides, catalyzes a rate-limiting reaction in the expression of poly-N-acetyllactosamine chains, especially in pheochromocytoma cells ?
-
 ?

Organism

Organism UniProt Comment Textmining
Rattus norvegicus
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
PC-12 cell pheochromacytoma cells, nerve growth factor stimulated cells with reduced GnT-i activity compared to unstimulated cells Rattus norvegicus
-
PC-12D cell new subline of pheochromacytoma cells PC-12, low GnT-i activity Rattus norvegicus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
 Rattus norvegicus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDP-N-acetyl-D-glucosamine + Galbeta(1-4)GlcNAcbeta(1-4)GlcNAc-2-aminopyridine
-
 Rattus norvegicus UDP + GlcNAcbeta(1-3)Galbeta(1-4)GlcNAcbeta(1-4)GlcNAc-2-aminopyridine
-
 ?
UDP-N-acetyl-D-glucosamine + poly-N-acetyllactosamine biosynthesis of poly-N-acetyllactosamine chains on N-linked oligosaccharides, catalyzes a rate-limiting reaction in the expression of poly-N-acetyllactosamine chains, especially in pheochromocytoma cells Rattus norvegicus ?
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Rattus norvegicus