Cloned (Comment) | Organism |
---|---|
cDNA from melanoma and colonic cancer cells is cloned and overexpressed in human Burkitt lymphoma Namalwa KJM-1 cells overexpressing the i-antigen, nucleotide and amino acid sequence of the 415-amino acids protein, cDNA fragment encoding the stem region plus putative catalytic domain of iGnT fused to protein A is expressed in COS-1 cells | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
Golgi apparatus | stem region and large catalytic domain reside presumably in the Golgi lumen | Homo sapiens | 5794 | - |
membrane | type II membrane protein topology, longest transmembrane domain among glycosyltransferases cloned so far | Homo sapiens | 16020 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
47125 | - |
x * 47125, calculated from the amino acid sequence, mature N-glycosylated iGnT: may be approximately 53 kDa | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-N-acetyl-D-glucosamine + beta-D-galactosyl-1,4-N-acetyl-D-glucosaminyl-R | Homo sapiens | enzyme is essential for the formation of poly-N-acetyllactosamines and the i-antigen, responsible for the formation of GlcNAcbeta(1-3)Galbeta(1-4)GlcNAc-R structure and poly-N-acetyllactosamine extension | UDP + N-acetyl-beta-D-glucosaminyl-1,3-beta-D-galactosyl-1,4-N-acetyl-D-glucosaminyl-R | - |
? | |
UDP-N-acetyl-D-glucosamine + poly-N-acetyllactosamine | Homo sapiens | - |
? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | O43505 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | 2 potential N-glycosylation sites | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
brain | highly expressed in fetal and adult brain | Homo sapiens | - |
kidney | highly expressed in fetal kidney | Homo sapiens | - |
additional information | enzyme is expressed ubiquitously in various adult tissues | Homo sapiens | - |
SW-1116 cell | colonic carcinoma cell line | Homo sapiens | - |
WM-266-4 cell | human melanoma cell line | Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | no acceptor: Galbeta(1-3)GlcNAcbeta(1-3)Galbeta(1-4)Glc | Homo sapiens | ? | - |
? | |
UDP-N-acetyl-D-glucosamine + beta-D-galactosyl-1,4-N-acetyl-D-glucosaminyl-R | iGnT has a unique characteristic in binding to acceptor substrates, preferentially adding poly-N-acetyllactosamine to membrane glycoproteins | Homo sapiens | UDP + N-acetyl-beta-D-glucosaminyl-1,3-beta-D-galactosyl-1,4-N-acetyl-D-glucosaminyl-R | - |
? | |
UDP-N-acetyl-D-glucosamine + beta-D-galactosyl-1,4-N-acetyl-D-glucosaminyl-R | enzyme is essential for the formation of poly-N-acetyllactosamines and the i-antigen, responsible for the formation of GlcNAcbeta(1-3)Galbeta(1-4)GlcNAc-R structure and poly-N-acetyllactosamine extension | Homo sapiens | UDP + N-acetyl-beta-D-glucosaminyl-1,3-beta-D-galactosyl-1,4-N-acetyl-D-glucosaminyl-R | - |
? | |
UDP-N-acetyl-D-glucosamine + Galbeta(1-4)GlcNAcbeta(1-3)Galbeta(1-4)Glc | - |
Homo sapiens | UDP + GlcNAcbeta(1-3)Galbeta(1-4)GlcNAcbeta(1-3)Galbeta(1-4)Glc | - |
? | |
UDP-N-acetyl-D-glucosamine + poly-N-acetyllactosamine | - |
Homo sapiens | ? | - |
? | |
UDP-N-acetylglucosamine + asialo-alpha1-acid glycoprotein | - |
Homo sapiens | UDP + N-acetylglucosaminylated asialo-alpha1-acid glycoprotein | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 47125, calculated from the amino acid sequence, mature N-glycosylated iGnT: may be approximately 53 kDa | Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |