Cloned (Comment) | Organism |
---|---|
expression in COS-7 cells, localization in membranes | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | slightly, 12% inhibition at 10 mM | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.36 | - |
Galbeta1,4(SO3-,6)-GlcNAcbeta1,3-Galbeta1,4(SO3-,6)-GlcNAc | recombinant enzyme, pH 7.2, 37°C | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | about one third of the activity compared to Mn2+, recombinant enzyme | Homo sapiens | |
Mg2+ | about one third of the activity compared to Mn2+, recombinant enzyme | Homo sapiens | |
Mn2+ | required for activity, recombinant enzyme | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Homo sapiens | enzyme is in volved in biosynthesis of keratan sulfate, overview, enzyme is an anti-migration factor for a lung cancer cell line | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | enzyme is in volved in biosynthesis of keratan sulfate, overview, enzyme is an anti-migration factor for a lung cancer cell line | Homo sapiens | ? | - |
? | |
additional information | substrate specificity, enzyme acts efficiently on keratan sulfate-related glycans, while lacto-N-tetraose and lacto-N-neo-tetraose are poor substrates, overview, product analysis | Homo sapiens | ? | - |
? | |
UDP-N-acetyl-D-glucosamine + Galbeta1,4(SO3-,6)-GlcNAcbeta1,3(SO3-,6)-Galbeta1,4(SO3-,6)-GlcNAc | i.e. L2L4, 72% activity compared to L2L2 | Homo sapiens | UDP + GlcNAcbeta1,3-Galbeta1,4(SO3-,6)-GlcNAcbeta1,3(SO3-,6)-Galbeta1,4(SO3-,6)-GlcNAc | - |
? | |
UDP-N-acetyl-D-glucosamine + Galbeta1,4(SO3-,6)-GlcNAcbeta1,3-Galbeta1,4(SO3-,6)-GlcNAc | i.e. L2L2, a 6-O-sulfated keratan sulfate, best substrate | Homo sapiens | UDP + GlcNAcbeta1,3-Galbeta1,4(SO3-,6)GlcNAcbeta1,3-Galbeta1,4(SO3-,6)-GlcNAc | - |
? | |
UDP-N-acetyl-D-glucosamine + Galbeta1,4(SO3-,6)-GlcNAcbeta1,3-Galbeta1,4(SO3-,6)-GlcNAc | i.e. L2L2, a 6-O-sulfated keratan sulfate, best substrate | Homo sapiens | UDP + GlcNAcbeta1,3-Galbeta1,4(SO3-,6)GlcNAcbeta1,3Galbeta1,4(SO3-,6)-GlcNAc | - |
? |
Synonyms | Comment | Organism |
---|---|---|
beta1,3-N-acetylglucosaminyltransferase-7 | - |
Homo sapiens |
beta3Gn-T7 | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | 7 | - |
Homo sapiens |