Cloned (Comment) | Organism |
---|---|
gene otsA, cloned from genetic library, DNA and amino acid sequence determination and analysis, overexpression in Escherichia coli strain BL21 | Thermus thermophilus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.5 | - |
UDP-glucose | recombinant enzyme, pH 7.0, 80°C, in presence of Mg2+ | Thermus thermophilus | |
0.8 | - |
D-glucose 6-phosphate | recombinant enzyme, pH 7.0, 80°C, in presence of Mg2+ | Thermus thermophilus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Co2+ | stimulates | Thermus thermophilus | |
Mg2+ | stimulates | Thermus thermophilus | |
additional information | no requirement for divalent cations for activity | Thermus thermophilus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
52000 | - |
x * 52000, recombinant enzyme, SDS-PAGE | Thermus thermophilus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-alpha-D-glucose + D-glucose 6-phosphate | Thermus thermophilus | first step in one of 2 existing trehalose biosynthesis pathways, plays a role in osmoadaptation | UDP + alpha,alpha-trehalose 6-phosphate | - |
? | |
UDP-alpha-D-glucose + D-glucose 6-phosphate | Thermus thermophilus RQ-1 | first step in one of 2 existing trehalose biosynthesis pathways, plays a role in osmoadaptation | UDP + alpha,alpha-trehalose 6-phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermus thermophilus | Q7WUI7 | gene otsA | - |
Thermus thermophilus RQ-1 | Q7WUI7 | gene otsA | - |
Purification (Comment) | Organism |
---|---|
recombinant enzyme from Escherichia coli | Thermus thermophilus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
20.6 | - |
purified recombinant enzyme, substrates GDP-glucose and D-glucose 6-phosphate | Thermus thermophilus |
95 | - |
purified recombinant enzyme, substrates ADP-glucose and D-glucose 6-phosphate | Thermus thermophilus |
106.7 | - |
purified recombinant enzyme, substrates TDP-glucose and D-glucose 6-phosphate | Thermus thermophilus |
149.6 | - |
purified recombinant enzyme, substrates UDP-glucose and D-glucose 6-phosphate | Thermus thermophilus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ADP-alpha-D-glucose + D-glucose 6-phosphate | less effective than UDP-glucose | Thermus thermophilus | ADP + alpha,alpha-trehalose 6-phosphate | - |
? | |
ADP-alpha-D-glucose + D-glucose 6-phosphate | less effective than UDP-glucose | Thermus thermophilus RQ-1 | ADP + alpha,alpha-trehalose 6-phosphate | - |
? | |
GDP-alpha-D-glucose + D-glucose 6-phosphate | much less effective than UDP-glucose | Thermus thermophilus | GDP + alpha,alpha-trehalose 6-phosphate | - |
? | |
GDP-alpha-D-glucose + D-glucose 6-phosphate | much less effective than UDP-glucose | Thermus thermophilus RQ-1 | GDP + alpha,alpha-trehalose 6-phosphate | - |
? | |
additional information | donor substrate specificity in descending order: UDP-glucose, TDP-glucose, ADP-glucose, GDP-glucose, no activity with mannose-6-phosphate, fructose 6-phosphate, or glucose 1,6-diphosphate as acceptors | Thermus thermophilus | ? | - |
? | |
additional information | donor substrate specificity in descending order: UDP-glucose, TDP-glucose, ADP-glucose, GDP-glucose, no activity with mannose-6-phosphate, fructose 6-phosphate, or glucose 1,6-diphosphate as acceptors | Thermus thermophilus RQ-1 | ? | - |
? | |
TDP-alpha-D-glucose + D-glucose 6-phosphate | less effective than UDP-glucose | Thermus thermophilus | TDP + alpha,alpha-trehalose 6-phosphate | - |
? | |
UDP-alpha-D-glucose + D-glucose 6-phosphate | first step in one of 2 existing trehalose biosynthesis pathways, plays a role in osmoadaptation | Thermus thermophilus | UDP + alpha,alpha-trehalose 6-phosphate | - |
? | |
UDP-alpha-D-glucose + D-glucose 6-phosphate | best substrates, absolutely specific for D-glucose 6-phosphate | Thermus thermophilus | UDP + alpha,alpha-trehalose 6-phosphate | - |
? | |
UDP-alpha-D-glucose + D-glucose 6-phosphate | first step in one of 2 existing trehalose biosynthesis pathways, plays a role in osmoadaptation | Thermus thermophilus RQ-1 | UDP + alpha,alpha-trehalose 6-phosphate | - |
? | |
UDP-alpha-D-glucose + D-glucose 6-phosphate | best substrates, absolutely specific for D-glucose 6-phosphate | Thermus thermophilus RQ-1 | UDP + alpha,alpha-trehalose 6-phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 52000, recombinant enzyme, SDS-PAGE | Thermus thermophilus |
Synonyms | Comment | Organism |
---|---|---|
TPS | - |
Thermus thermophilus |
trehalose-6-phosphate synthase | - |
Thermus thermophilus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
98 | - |
- |
Thermus thermophilus |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 100 | about half-maximal activity at 40°C, 80% of maximal activity at 100°C, inactive at 30°C | Thermus thermophilus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
80 | - |
half-life: 193 min | Thermus thermophilus |
90 | - |
half-life: 54 min | Thermus thermophilus |
100 | - |
half-life: 31 min | Thermus thermophilus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
- |
Thermus thermophilus |