Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Actinoplanes sp. |
General Stability | Organism |
---|---|
in phosphate or acetate buffers, the activity of TpS1 is decreased to 25% and 30%, respectively, of activity in Tris-HCl buffer | Actinoplanes sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | - |
Actinoplanes sp. | |
additional information | not inhibitory: 2-mercaptoethanol | Actinoplanes sp. |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | 95% stimulation | Actinoplanes sp. | |
Mn2+ | 20% stimulation | Actinoplanes sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
51000 | - |
x * 51000, calculated | Actinoplanes sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Actinoplanes sp. | A8QX04 | - |
- |
Actinoplanes sp. SN223/29 | A8QX04 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ADP-alpha-D-glucose + D-glucose 6-phosphate | 4% of the activity with UDP-glucose | Actinoplanes sp. | ADP + alpha,alpha-1,1-trehalose 6-phosphate | - |
? | |
ADP-alpha-D-glucose + D-glucose 6-phosphate | 4% of the activity with UDP-glucose | Actinoplanes sp. SN223/29 | ADP + alpha,alpha-1,1-trehalose 6-phosphate | - |
? | |
GDP-alpha-D-glucose + D-glucose 6-phosphate | 23% of the activity with UDP-glucose | Actinoplanes sp. | GDP + alpha,alpha-1,1-trehalose 6-phosphate | - |
? | |
GDP-alpha-D-glucose + D-glucose 6-phosphate | 23% of the activity with UDP-glucose | Actinoplanes sp. SN223/29 | GDP + alpha,alpha-1,1-trehalose 6-phosphate | - |
? | |
additional information | no substrate: CDP-glucose, fructose 6-phosphate | Actinoplanes sp. | ? | - |
? | |
additional information | no substrate: CDP-glucose, fructose 6-phosphate | Actinoplanes sp. SN223/29 | ? | - |
? | |
UDP-alpha-D-glucose + D-glucose 6-phosphate | - |
Actinoplanes sp. | UDP + alpha,alpha-1,1-trehalose 6-phosphate | - |
? | |
UDP-alpha-D-glucose + D-glucose 6-phosphate | - |
Actinoplanes sp. SN223/29 | UDP + alpha,alpha-1,1-trehalose 6-phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 51000, calculated | Actinoplanes sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
- |
Actinoplanes sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Actinoplanes sp. |