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Literature summary for 2.4.1.15 extracted from

  • Shi, Q.; Chung, J.S.
    Trehalose metabolism in the blue crab Callinectes sapidus: isolation of multiple structural cDNA isoforms of trehalose-6-phosphate synthase and their expression in muscles (2014), Gene, 536, 105-113.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
isozyme TPS1, DNA and amino acid sequence determination and analysis, sequence comparison and phylogenic analysis, the full-length cDNA sequence of four structural isoforms of trehalose-6-phosphate synthase (TPS) is isolated fromchela muscles of an adult female crabl. Two isoforms of Callinectes sapidus TPS encode functional domains of TPS and trehalose-6-phosphorylase, TPP, EC 2.4.1.216, in tandem as a fused gene product of Escherichia coli Ost A and Ost B. The other two isoforms contain only a single TPS domain. Quantitative RT-PCR isozyme expression analysis Callinectes sapidus
isozyme TPS1a, DNA and amino acid sequence determination and analysis, sequence comparison and phylogenic analysis, the full-length cDNA sequence of four structural isoforms of trehalose-6-phosphate synthase (TPS) is isolated fromchela muscles of an adult female crab. Two isoforms of Callinectes sapidus TPS encode functional domains of TPS and trehalose-6-phosphorylase, TPP, EC 2.4.1.216, in tandem as a fused gene product of Escherichia coli Ost A and Ost B. The other two isoforms contain only a single TPS domain. Quantitative RT-PCR isozyme expression analysis Callinectes sapidus
isozyme TPS1b, DNA and amino acid sequence determination and analysis, sequence comparison and phylogenic analysis, the full-length cDNA sequence of four structural isoforms of trehalose-6-phosphate synthase (TPS) is isolated fromchela muscles of an adult female crab. Two isoforms of Callinectes sapidus TPS encode functional domains of TPS and trehalose-6-phosphorylase, TPP, EC 2.4.1.216, in tandem as a fused gene product of Escherichia coli Ost A and Ost B. The other two isoforms contain only a single TPS domain. Quantitative RT-PCR isozyme expression analysis Callinectes sapidus
isozyme TPS1c, DNA and amino acid sequence determination and analysis, sequence comparison and phylogenic analysis, the full-length cDNA sequence of four structural isoforms of trehalose-6-phosphate synthase (TPS) is isolated fromchela muscles of an adult female crab. Two isoforms of Callinectes sapidus TPS encode functional domains of TPS and trehalose-6-phosphorylase, TPP, EC 2.4.1.216, in tandem as a fused gene product of Escherichia coli Ost A and Ost B. The other two isoforms contain only a single TPS domain. Quantitative RT-PCR isozyme expression analysis Callinectes sapidus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
UDP-glucose + D-glucose 6-phosphate Callinectes sapidus
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UDP + alpha,alpha-trehalose 6-phosphate
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?

Organism

Organism UniProt Comment Textmining
Callinectes sapidus E1U2Z7 isozyme 1; collected from the upper Chesapeake Bay, China
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Callinectes sapidus E1U2Z8 isozyme 1a; collected from the upper Chesapeake Bay, China
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Callinectes sapidus E1U2Z9 isozyme 1b; collected from the upper Chesapeake Bay, China
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Callinectes sapidus E1U300 isozyme 1c; collected from the upper Chesapeake Bay, China
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Source Tissue

Source Tissue Comment Organism Textmining
basal swimming muscle
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Callinectes sapidus
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claw muscle in both males and females, the darker muscles exhibit greater amounts of trehalose, trehalose 6-phosphate synthase, and trehalase activities than the light colored Callinectes sapidus
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additional information ubiquitous expression of CasTPS in most tissues Callinectes sapidus
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDP-glucose + D-glucose 6-phosphate
-
Callinectes sapidus UDP + alpha,alpha-trehalose 6-phosphate
-
?

Subunits

Subunits Comment Organism
More isozyme domain structure, overview Callinectes sapidus
More isozyme domain structure, overview. The isozyme 1 encodes both a putative TPS and a TPP domain in tandem Callinectes sapidus
More isozyme domain structure, overview. The isozyme 1b encodes both a putative TPS and a TPP domain in tandem Callinectes sapidus

Synonyms

Synonyms Comment Organism
TPS
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Callinectes sapidus
trehalose-6-phosphate synthase
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Callinectes sapidus

General Information

General Information Comment Organism
additional information differences in muscle trehalose content in female and male animals due to polymorphisms Callinectes sapidus