Cloned (Comment) | Organism |
---|---|
isozyme TPS1, DNA and amino acid sequence determination and analysis, sequence comparison and phylogenic analysis, the full-length cDNA sequence of four structural isoforms of trehalose-6-phosphate synthase (TPS) is isolated fromchela muscles of an adult female crabl. Two isoforms of Callinectes sapidus TPS encode functional domains of TPS and trehalose-6-phosphorylase, TPP, EC 2.4.1.216, in tandem as a fused gene product of Escherichia coli Ost A and Ost B. The other two isoforms contain only a single TPS domain. Quantitative RT-PCR isozyme expression analysis | Callinectes sapidus |
isozyme TPS1a, DNA and amino acid sequence determination and analysis, sequence comparison and phylogenic analysis, the full-length cDNA sequence of four structural isoforms of trehalose-6-phosphate synthase (TPS) is isolated fromchela muscles of an adult female crab. Two isoforms of Callinectes sapidus TPS encode functional domains of TPS and trehalose-6-phosphorylase, TPP, EC 2.4.1.216, in tandem as a fused gene product of Escherichia coli Ost A and Ost B. The other two isoforms contain only a single TPS domain. Quantitative RT-PCR isozyme expression analysis | Callinectes sapidus |
isozyme TPS1b, DNA and amino acid sequence determination and analysis, sequence comparison and phylogenic analysis, the full-length cDNA sequence of four structural isoforms of trehalose-6-phosphate synthase (TPS) is isolated fromchela muscles of an adult female crab. Two isoforms of Callinectes sapidus TPS encode functional domains of TPS and trehalose-6-phosphorylase, TPP, EC 2.4.1.216, in tandem as a fused gene product of Escherichia coli Ost A and Ost B. The other two isoforms contain only a single TPS domain. Quantitative RT-PCR isozyme expression analysis | Callinectes sapidus |
isozyme TPS1c, DNA and amino acid sequence determination and analysis, sequence comparison and phylogenic analysis, the full-length cDNA sequence of four structural isoforms of trehalose-6-phosphate synthase (TPS) is isolated fromchela muscles of an adult female crab. Two isoforms of Callinectes sapidus TPS encode functional domains of TPS and trehalose-6-phosphorylase, TPP, EC 2.4.1.216, in tandem as a fused gene product of Escherichia coli Ost A and Ost B. The other two isoforms contain only a single TPS domain. Quantitative RT-PCR isozyme expression analysis | Callinectes sapidus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-glucose + D-glucose 6-phosphate | Callinectes sapidus | - |
UDP + alpha,alpha-trehalose 6-phosphate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Callinectes sapidus | E1U2Z7 | isozyme 1; collected from the upper Chesapeake Bay, China | - |
Callinectes sapidus | E1U2Z8 | isozyme 1a; collected from the upper Chesapeake Bay, China | - |
Callinectes sapidus | E1U2Z9 | isozyme 1b; collected from the upper Chesapeake Bay, China | - |
Callinectes sapidus | E1U300 | isozyme 1c; collected from the upper Chesapeake Bay, China | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
basal swimming muscle | - |
Callinectes sapidus | - |
claw muscle | in both males and females, the darker muscles exhibit greater amounts of trehalose, trehalose 6-phosphate synthase, and trehalase activities than the light colored | Callinectes sapidus | - |
additional information | ubiquitous expression of CasTPS in most tissues | Callinectes sapidus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-glucose + D-glucose 6-phosphate | - |
Callinectes sapidus | UDP + alpha,alpha-trehalose 6-phosphate | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | isozyme domain structure, overview | Callinectes sapidus |
More | isozyme domain structure, overview. The isozyme 1 encodes both a putative TPS and a TPP domain in tandem | Callinectes sapidus |
More | isozyme domain structure, overview. The isozyme 1b encodes both a putative TPS and a TPP domain in tandem | Callinectes sapidus |
Synonyms | Comment | Organism |
---|---|---|
TPS | - |
Callinectes sapidus |
trehalose-6-phosphate synthase | - |
Callinectes sapidus |
General Information | Comment | Organism |
---|---|---|
additional information | differences in muscle trehalose content in female and male animals due to polymorphisms | Callinectes sapidus |