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Literature summary for 2.4.1.150 extracted from

  • Chen, G.Y.; Kurosawa, N.; Muramatsu, T.
    A novel variant form of murine beta-1,6-N-acetylglucosaminyltransferase forming branches in poly-N-acetyllactosamines (2000), Glycobiology, 10, 1001-1011.
    View publication on PubMed
Search for more literature for 2.4.1.150

Cloned(Commentary)

Cloned (Comment) Organism
2 isoforms IGnT A and IGnT B are produced by alternative splicing of the IGnT gene, the C-terminal 1/4 of IGnT B is identical to that of IGnT A Mus musculus
IGnT A is cloned Mus musculus
IGnT B is cloned from mouse liver cDNA, nucleotide and amino acid sequence, exon organization of the IGnT gene, expression of IGnT A and B in COS-7 and CHO cells Mus musculus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.44
-
 Galbeta(1-4)GlcNAcbeta(1-3)Galbeta(1-4)Glc IGnT B Mus musculus
0.52
-
 NeuAcalpha(2-6)Galbeta(1-4)GlcNacbeta(1-3)Galbeta(1-4)Glc IGnT B Mus musculus
0.55
-
 Galbeta(1-4)GlcNAcbeta(1-3)Galbeta(1-4)GlcNAcbeta(1-3)Galbeta(1-4)Glc IGnT B Mus musculus

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane type II transmembrane topology Mus musculus 16020
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
UDP-N-acetyl-D-glucosamine + poly-N-acetyllactosamine Mus musculus forms branches in poly-N-acetyllactosamines, which bear the I blood group antigen ?
-
 ?

Organism

Organism UniProt Comment Textmining
Mus musculus A2IQH4 IGnT B, 2 isoenzymes: IGnT A and B
-
Mus musculus A2IQH5 IGnT A, 2 isoenzymes: IGnT A and B
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Source Tissue

Source Tissue Comment Organism Textmining
ES-D3 cell D-3 embryonic stem cells moderately express IGnT A and B Mus musculus
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intestine strong expression of IGnT A and B in adults Mus musculus
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kidney strong expression of IGnT A and B in adults Mus musculus
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liver strong expression of IGnT A and B in adults Mus musculus
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mammary gland moderate expression of IGnT A and B Mus musculus
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P-19 cell P-19 embryonal carcinoma cells moderately express IGnT A and B Mus musculus
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submaxillary gland moderate expression of IGnT A and B Mus musculus
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Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 when expressed as proteins IGnT B shows higher specific activity than IGnT A Mus musculus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information 2 isoforms IGnT A and IGnT B, C-terminal 1/4 of IGnT B is identical to that of IGnT A, the rest of the sequences shows 63% identity Mus musculus ?
-
 ?
additional information generally, oligosaccharides with the Galbeta(1-4)GlcNAcbeta(1-3)Galbeta(1-4)Glc(Nac) sequence serve as good substrates, addition of Galalpha(1-3) or sialic acid alpha(2-6) to the non-reducing end gelactose enhances the acceptor activity, while sialic acid alpha2-3 linkage shows a repressive effect, no substrate: N-acetyl-beta-D-glucosaminyl-1,3-beta-D-galactosyl-1,4-beta-D-glucose Mus musculus ?
-
 ?
UDP-N-acetyl-D-glucosamine + Galalpha(1-3)Galbeta(1-4)GlcNAcbeta(1-3)Galbeta(1-4)Glc IGnT A: at 256% of the rate with lacto-N-neotetraose Mus musculus ?
-
 ?
UDP-N-acetyl-D-glucosamine + Galalpha(1-3)Galbeta(1-4)GlcNAcbeta(1-3)Galbeta(1-4)Glc IGnT B: at 148% of the rate with lacto-N-neotetraose Mus musculus ?
-
 ?
UDP-N-acetyl-D-glucosamine + Galbeta(1-3)GlcNAcbeta(1-3)Galbeta(1-4)Glc poor substrate Mus musculus ?
-
 ?
UDP-N-acetyl-D-glucosamine + Galbeta(1-3)GlcNAcbeta(1-3)Galbeta(1-4)Glc IGnT B: at 6% of the rate with lacto-N-neotetraose Mus musculus ?
-
 ?
UDP-N-acetyl-D-glucosamine + Galbeta(1-3)GlcNAcbeta(1-3)Galbeta(1-4)Glc IGnT A: at 4% of the rate with lacto-N-neotetraose Mus musculus ?
-
 ?
UDP-N-acetyl-D-glucosamine + Galbeta(1-3)GlcNAcbeta(1-3)Galbeta(1-4)GlcNAcbeta(1-3)Galbeta(1-4)Glc poor substrate Mus musculus ?
-
 ?
UDP-N-acetyl-D-glucosamine + Galbeta(1-3)GlcNAcbeta(1-3)Galbeta(1-4)GlcNAcbeta(1-3)Galbeta(1-4)Glc IGnT B: at 10% of the rate with lacto-N-neotetraose Mus musculus ?
-
 ?
UDP-N-acetyl-D-glucosamine + Galbeta(1-3)GlcNAcbeta(1-3)Galbeta(1-4)GlcNAcbeta(1-3)Galbeta(1-4)Glc IGnT A: at 6% of the rate with lacto-N-neotetraose Mus musculus ?
-
 ?
UDP-N-acetyl-D-glucosamine + Galbeta(1-4)(Fucalpha(1-3))GlcNAcbeta(1-3)Galbeta(1-4)Glc very poor substrate Mus musculus ?
-
 ?
UDP-N-acetyl-D-glucosamine + Galbeta(1-4)GlcNAcbeta(1-3)Galbeta(1-4)Glc i.e. lacto-N-neotetraose, IGnT B forms branch in the internal Gal residue Mus musculus UDP + Galbeta(1-4)GlcNAcbeta(1-3)(GlcNAcbeta(1-6))Galbeta(1-4)Glc no transfer to the terminal Gal residue ?
UDP-N-acetyl-D-glucosamine + Galbeta(1-4)GlcNAcbeta(1-3)Galbeta(1-4)GlcNAcbeta(1-3)Galbeta(1-4)Glc IGnT B: at 181% of the rate with lacto-N-neotetraose Mus musculus UDP + Galbeta(1-4)GlcNAcbeta(1-3)(GlcNAcbeta(1-6))Galbeta(1-4)GlcNAcbeta(1-3)Galbeta(1-4)Glc IGnT B: major product, minor product is Galbeta(1-4)GlcNAcbeta(1-3)Galbeta(1-4)GlcNAcbeta(1-3)(GlcNAcbeta(1-6))Galbeta(1-4)Glc, IGnT B forms beta-1,6 branch in both of the internal galactosyl residues, prolonged incubation results in di-branched oligosaccharide ?
UDP-N-acetyl-D-glucosamine + Galbeta(1-4)GlcNAcbeta(1-3)Galbeta(1-4)GlcNAcbeta(1-3)Galbeta(1-4)Glc IGnT A: at 170% of the rate with lacto-N-neotetraose Mus musculus UDP + Galbeta(1-4)GlcNAcbeta(1-3)(GlcNAcbeta(1-6))Galbeta(1-4)GlcNAcbeta(1-3)Galbeta(1-4)Glc
-
 ?
UDP-N-acetyl-D-glucosamine + Galbeta(1-4)GlcNAcbeta(1-3)Galbeta(1-4)GlcNAcbeta(1-3)Galbeta(1-4)Glc i.e. para-lacto-N-neohexaose Mus musculus UDP + Galbeta(1-4)GlcNAcbeta(1-3)(GlcNAcbeta(1-6))Galbeta(1-4)GlcNAcbeta(1-3)Galbeta(1-4)Glc
-
 ?
UDP-N-acetyl-D-glucosamine + Galbeta(1-4)GlcNAcbeta(1-3)Galbeta(1-4)GlcNAcbeta(1-3)Galbeta(1-4)Glc i.e. para-lacto-N-neohexaose Mus musculus UDP + Galbeta(1-4)GlcNAcbeta(1-3)(GlcNAcbeta(1-6))Galbeta(1-4)GlcNAcbeta(1-3)Galbeta(1-4)Glc IGnT B: major product, minor product is Galbeta(1-4)GlcNAcbeta(1-3)Galbeta(1-4)GlcNAcbeta(1-3)(GlcNAcbeta(1-6))Galbeta(1-4)Glc, IGnT B forms beta-1,6 branch in both of the internal galactosyl residues, prolonged incubation results in di-branched oligosaccharide ?
UDP-N-acetyl-D-glucosamine + Galbeta(1-4)GlcNAcbeta(1-3)Galbeta(1-4)GlcNAcbeta(1-3)Galbeta(1-4)GlcNAc IGnT B: at 164% of the rate with lacto-N-neotetraose Mus musculus UDP + Galbeta(1-4)GlcNAcbeta(1-3)(GlcNAcbeta(1-6))Galbeta(1-4)GlcNAcbeta(1-3)Galbeta(1-4)GlcNAc
-
 ?
UDP-N-acetyl-D-glucosamine + Galbeta(1-4)GlcNAcbeta(1-3)Galbeta(1-4)GlcNAcbeta(1-3)Galbeta(1-4)GlcNAc IGnT A: at 194% of the rate with lacto-N-neotetraose Mus musculus UDP + Galbeta(1-4)GlcNAcbeta(1-3)(GlcNAcbeta(1-6))Galbeta(1-4)GlcNAcbeta(1-3)Galbeta(1-4)GlcNAc
-
 ?
UDP-N-acetyl-D-glucosamine + GlcNAcbeta(1-3)Galbeta(1-4)GlcNAcbeta(1-3)Galbeta(1-4)Glc IGnT A: at 22% of the rate with lacto-N-neotetraose Mus musculus UDP + GlcNAcbeta(1-3)Galbeta(1-4)GlcNAcbeta(1-3)(GlcNAcbeta(1-6))Galbeta(1-4)Glc only product ?
UDP-N-acetyl-D-glucosamine + GlcNAcbeta(1-3)Galbeta(1-4)GlcNAcbeta(1-3)Galbeta(1-4)Glc IGnT B: at 41% of the rate with lacto-N-neotetraose Mus musculus UDP + GlcNAcbeta(1-3)Galbeta(1-4)GlcNAcbeta(1-3)(GlcNAcbeta(1-6))Galbeta(1-4)Glc only product ?
UDP-N-acetyl-D-glucosamine + NeuAcalpha(2-3)Galbeta(1-4)GlcNacbeta(1-3)Galbeta(1-4)Glc IGnT B: at 123% of the rate with lacto-N-neotetraose Mus musculus ?
-
 ?
UDP-N-acetyl-D-glucosamine + NeuAcalpha(2-3)Galbeta(1-4)GlcNacbeta(1-3)Galbeta(1-4)Glc IGnT A: at 35% of the rate with lacto-N-neotetraose Mus musculus ?
-
 ?
UDP-N-acetyl-D-glucosamine + NeuAcalpha(2-6)Galbeta(1-4)GlcNacbeta(1-3)Galbeta(1-4)Glc IGnT A: at 256% of the rate with lacto-N-neotetraose Mus musculus ?
-
 ?
UDP-N-acetyl-D-glucosamine + NeuAcalpha(2-6)Galbeta(1-4)GlcNacbeta(1-3)Galbeta(1-4)Glc IGnT B: at 148% of the rate with lacto-N-neotetraose Mus musculus ?
-
 ?
UDP-N-acetyl-D-glucosamine + poly-N-acetyllactosamine forms branches in poly-N-acetyllactosamines Mus musculus ?
-
 ?
UDP-N-acetyl-D-glucosamine + poly-N-acetyllactosamine forms branches in poly-N-acetyllactosamines, which bear the I blood group antigen Mus musculus ?
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Mus musculus