Activating Compound | Comment | Organism | Structure |
---|---|---|---|
beta-mercaptoethanol | - |
Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.043 | - |
GDP-fucose | - |
Homo sapiens | |
0.53 | - |
N-acetyl-beta-lactosamine | - |
Homo sapiens | |
0.76 | - |
lacto-N-neotetraose | - |
Homo sapiens | |
0.96 | - |
NeuAcalpha2-3Galbeta1-4GlcNAc | - |
Homo sapiens |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
45000 | - |
gel filtration | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
using ammonium sulfate precipitation, hydrophobic chromatography in phenyl-Sepharose, ion-exchange chromatography on sulfopropyl-Sepharose, affinity chromatography on GDP-heanolamine-Sepharose, and finally high pressure liquid chromatography gel filtration | Homo sapiens |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
GDP-fucose + Fucalpha1-2Galbeta1-4GlcNAc | - |
Homo sapiens | GDP + Fucalpha1-2Galbeta1-4(Fucalpha1-3)GlcNAc | - |
? | |
GDP-fucose + lacto-N-neotetraose | - |
Homo sapiens | GDP + ? | - |
? | |
GDP-fucose + NeuAcalpha2-3Galbeta1-4GlcNAc | - |
Homo sapiens | GDP + NeuAcalpha2-3Galbeta1-4(Fucalpha1-3)GlcNAc | - |
? | |
GDP-L-fucose + N4-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-3)-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-6)]-beta-D-mannosyl-(1-4)-N-acetyl-beta-D-glucosaminyl-(1-4)-N-acetyl-beta-D-glucosaminyl]asparagine | - |
Homo sapiens | GDP + N4-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-3)-[N-acetyl-beta-D-glucosaminyl-(1-2)-alpha-D-mannosyl-(1-6)]-beta-D-mannosyl-(1-4)-N-acetyl-beta-D-glucosaminyl-(1-4)-[alpha-L-fucosyl-(1-3)]-N-acetyl-beta-D-glucosaminyl]asparagine | - |
? | |
additional information | lactose and its fucose-substituted derivative are very poor acceptors for the enzyme | Homo sapiens | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 45000, SDS-PAGE | Homo sapiens |