Cloned (Comment) | Organism |
---|---|
cloning of rat Fuc-TVII gene which is expressed as two splice variants, but only the long one shows enzymatic activity, expression of the long splice variant in COS-7 cells | Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
tunicamycin | inhibits N-glycosylation of the enzyme in recombinant COS-7 cells which leads to inactivation of rFuc-TVII | Rattus norvegicus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | Q712G6 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | Tyr191 of rFuc-TVII is predicted to be O-glycosylated, three potential N-glycosylation sites at residues 86, 109, and 299, N-glycosylation is necessary for enzymatic activity | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
kidney | rFuc-TVII mRNA expression undetectable in kidney but it is strongly enhanced during acute inflammatory reaction induced by kidney allograft rejection | Rattus norvegicus | - |
lymph node | rFuc-TVII mRNA expression strongly detected in rat lymph node | Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.000017 | - |
substrate sialyl-N-acetyllactosamine | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
GDP-L-fucose + sialyl-alpha-2,3-N-acetyllactosamine | - |
Rattus norvegicus | GDP + NeuNAcalpha2-3Galbeta1-4[Fucalpha1-3]GlcNAc | - |
? |
Synonyms | Comment | Organism |
---|---|---|
rat fucosyltransferase VII | - |
Rattus norvegicus |
rFuc-TVII | - |
Rattus norvegicus |
rFUT7 | - |
Rattus norvegicus |