Literature summary for 2.4.1.152 extracted from

Salo, H.; Sievi, E.; Suntio, T.; Mecklin, M.; Mattila, P.; Renkonen, R.; Makarow, M.
Co-expression of two mammalian glycosyltransferases in the yeast cell wall allows synthesis of sLex (2005), FEMS Yeast Res., 5, 341-350.

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Application

Application	Comment	Organism
synthesis	recombinant yeast cells which provide an inexpensive, self-perpetuating source of fucosyltransferase activity immobilized in the cell wall, useful for in vitro synthesis of sLex	Homo sapiens

Cloned(Commentary)

Cloned (Comment)	Organism
expression in Pichia pastoris	Homo sapiens
expression in Saccharomyces cerevisiae	Homo sapiens

Protein Variants

Protein Variants	Comment	Organism
additional information	catalytic domain of alpha-1,3-fucosyltransferase (amino acids 37-441) are expressed in Saccharomyces cerevisiae and Pichia pastoris as a fusion protein HSP150 delta-FucTe consisting of HSP150delta (1-321) and catalytic domain of FucTe (37-441) to promote proper folding and secretion	Homo sapiens
additional information	Saccharomyces cerevisiae strain created co-expressing the catalytic domain of alpha-2,3-sialyltransferase and Fuc-Te in the cell wall, addition of N-acetyllactosamine, CMP-sialic acid and GDP-fucose results in the production of sLex	Homo sapiens

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
1.7	-	sialyl-alpha-2,3-N-acetyllactosamine	fusion protein Hsp150delta-FucTe	Homo sapiens

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
cell wall	hsp150delta-FucTe fusion protein remains mostly non-covalently attached to the cell wall	Homo sapiens	5618	-

Metals/Ions

Metals/Ions	Comment	Organism	Structure
MnCl2	glycosyltransferase assay with 20 mM MnCl2	Homo sapiens

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	-	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
GDP-fucose + sialyl-alpha-2,3-N-acetyllactosamine	-	Homo sapiens	GDP + NeuNAcalpha2-3Galbeta1-4[Fucalpha1-3]GlcNAc	-	?

Synonyms

Synonyms	Comment	Organism
alpha-13-fucosyltransferase VII	-	Homo sapiens
FucTe	-	Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
24	-	glycosyltransferase assay	Homo sapiens

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7	-	glycosyltransferase assay	Homo sapiens

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Release 2023.1 (January 2023)