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Literature summary for 2.4.1.152 extracted from

  • Ma, B.; Audette, G.F.; Lin, S.; Palcic, M.M.; Hazes, B.; Taylor, D.E.
    Purification, kinetic characterization, and mapping of the minimal catalytic domain and the key polar goups of Helicobacter pylori alpha-(1,3/1,4)-fucosyltransferases (2006), J. Biol. Chem., 281, 6385-6394.
    View publication on PubMed
Search for more literature for 2.4.1.152

Cloned(Commentary)

Cloned (Comment) Organism
-
 Helicobacter pylori
full-length and each truncated FucT (DELTAFucT) are expressed in Escherichia coli HMS174DE3 Helicobacter pylori

Protein Variants

Protein Variants Comment Organism
additional information with full length 11639FucT, 28% of total enzyme activities is localized in the soluble fraction. For alpha-(1,3/1,4)-fucosyltransferase(1–441), this increases to 45%, respectively, indicating that truncation of the C-terminal putative alpha-helices increases FucT solubility. Truncation mutant alpha-(1,3/1,4)-fucosyltransferase(10–447) completely loses activity Helicobacter pylori
additional information with full length UA948FucT, 20% of total enzyme activities is localized in the soluble fraction. For UA948(1-428), this increases to 47%, respectively, indicating that truncation of the C-terminal putative alpha-helices increases FucT solubility. UA948(1-364), a mutant with the entire heptad repeat region removed, exhibits extremely low levels of enzyme activity. This indicates that the heptad repeat region is essential for enzyme activity. Construct alpha-(1,3/1,4)-fucosyltransferase(1-371) has little enzyme activity. alpha-(1,3/1,4)-fucosyltransferase(10-434) and alpha-(1,3/1,4)-fucosyltransferase(1-434) completely lose activity Helicobacter pylori

General Stability

General Stability Organism
lyophilized alpha-(1,3/1,4)-fucosyltransferase(1-428) loses 23% of its activity after 3 month Helicobacter pylori

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0156
-
 GDP-beta-L-fucose cosubstrate: Galbeta1,4GlcNAc-O(CH2)8CO2CH3, truncated enzyme alpha-(1,3/1,4)-fucosyltransferase(1–441) Helicobacter pylori
0.0176
-
 GDP-beta-L-fucose cosubstrate: Galbeta1,4GlcNAc-O(CH2)8CO2CH3, truncated enzyme alpha-(1,3/1,4)-fucosyltransferase(1–428) Helicobacter pylori
0.0447
-
 GDP-beta-L-fucose cosubstrate: Galbeta1,4GlcNAc-O(CH2)8CO2CH3, full-length enzyme Helicobacter pylori
0.056
-
 GDP-beta-L-fucose cosubstrate: Galbeta1,4GlcNAc-O(CH2)8CO2CH3, full-length enzyme Helicobacter pylori
0.37
-
 Galbeta1,4GlcNAc-O(CH2)8CO2CH3 full-length enzyme Helicobacter pylori
0.39
-
 Galbeta1,4GlcNAc-O(CH2)8CO2CH3 truncated enzyme alpha-(1,3/1,4)-fucosyltransferase(1–441) Helicobacter pylori
1.5
-
 Galbeta1,4GlcNAc-O(CH2)8CO2CH3 full-length enzyme Helicobacter pylori
1.7
-
 Galbeta1,4GlcNAc-O(CH2)8CO2CH3 truncated enzyme alpha-(1,3/1,4)-fucosyltransferase(1–428) Helicobacter pylori

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
51200
-
 x * 51200, alpha-(1,3/1,4)-fucosyltransferase(1-428), calculated from sequence Helicobacter pylori
51200
-
 x * 51200, mutant alpha-(1,3/1,4)-fucosyltransferase (1–441), calculated from sequence Helicobacter pylori

Organism

Organism UniProt Comment Textmining
Helicobacter pylori
-
-
-
Helicobacter pylori NCTC11639
-
-
-
Helicobacter pylori UA948
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Helicobacter pylori
alpha-(1,3/1,4)-fucosyltransferase(1–428) with a His6 tag at the C terminus is purified Helicobacter pylori

Storage Stability

Storage Stability Organism
lyophilized alpha-(1,3/1,4)-fucosyltransferase(1–441) loses 38% activity after 3 months Helicobacter pylori
most stable at -80°C with or without glycerol supplementation Helicobacter pylori

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GDP-beta-L-fucose + 3-sulfo-Galbeta1,4GlcNAc 135% of the activity with Galbeta1,4GlcNAc Helicobacter pylori ?
-
 ?
GDP-beta-L-fucose + 3-sulfo-Galbeta1,4GlcNAc 72% of the activity with Galbeta1,4GlcNAc Helicobacter pylori ?
-
 ?
GDP-beta-L-fucose + 4-deoxy-Galbeta1,4GlcNAc 8% of the activity with Galbeta1,4GlcNAc Helicobacter pylori ?
-
 ?
GDP-beta-L-fucose + Fucalpha1,2Galbeta1,4GlcNAc 115% of the activity with Galbeta1,4GlcNAc Helicobacter pylori ?
-
 ?
GDP-beta-L-fucose + Fucalpha1,2Galbeta1,4GlcNAc 99% of the activity with Galbeta1,4GlcNAc Helicobacter pylori ?
-
 ?
GDP-beta-L-fucose + Galbeta1,4GlcNAc
-
 Helicobacter pylori ?
-
 ?
GDP-beta-L-fucose + NeuAcalpha2,3Galbeta1,4GlcNAc 32% of the activity with Galbeta1,4GlcNAc Helicobacter pylori ?
-
 ?
GDP-L-fucose + Galbeta(1,4)GlcNAc-O(CH2)8CO2CH3
-
 Helicobacter pylori GDP + Galbeta(1,4)(Fucalpha(1,3))GlcNAc-O(CH2)8CO2CH3
-
 ?
additional information the enzyme shows alpha1,3- and alpha1,4-fucosyltransferase activity Helicobacter pylori ?
-
 ?

Subunits

Subunits Comment Organism
? x * 51200, alpha-(1,3/1,4)-fucosyltransferase(1-428), calculated from sequence Helicobacter pylori
? x * 51200, mutant alpha-(1,3/1,4)-fucosyltransferase (1–441), calculated from sequence Helicobacter pylori

Synonyms

Synonyms Comment Organism
11639FucT
-
 Helicobacter pylori
alpha-(1,3/1,4)-fucosyltransferase
-
 Helicobacter pylori
alpha-(1,3/1,4)-FucT
-
 Helicobacter pylori
UA948FucT
-
 Helicobacter pylori

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1
-
 GDP-beta-L-fucose cosubstrate: Galbeta1,4GlcNAc-O(CH2)8CO2CH3, truncated enzyme alpha-(1,3/1,4)-fucosyltransferase(1–428) Helicobacter pylori
1.1
-
 Galbeta1,4GlcNAc-O(CH2)8CO2CH3 truncated enzyme alpha-(1,3/1,4)-fucosyltransferase(1–428) Helicobacter pylori
2.3
-
 GDP-beta-L-fucose cosubstrate: Galbeta1,4GlcNAc-O(CH2)8CO2CH3, truncated enzyme alpha-(1,3/1,4)-fucosyltransferase(1–428) Helicobacter pylori
2.3
-
 Galbeta1,4GlcNAc-O(CH2)8CO2CH3 truncated enzyme alpha-(1,3/1,4)-fucosyltransferase(1–428) Helicobacter pylori