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Literature summary for 2.4.1.152 extracted from

  • Brito, C.; Kandzia, S.; Graca, T.; Conradt, H.S.; Costa, J.
    Human fucosyltransferase IX: specificity towards N-linked glycoproteins and relevance of the cytoplasmic domain in intra-Golgi localization (2008), Biochimie, 90, 1279-1290.
    View publication on PubMed
Search for more literature for 2.4.1.152

Cloned(Commentary)

Cloned (Comment) Organism
FUT9, DNA and amino acid sequence determination and analysis, transient overexpression of His-tagged or V5-tagged wild-type and mutant FUT9 proteins in HeLa cells Homo sapiens

Protein Variants

Protein Variants Comment Organism
additional information construction of mutants FUT9dcyt, FUT9d6, FUT9S/A, subcellular localization analysis of recombinant FUT9wt and mutants in HeLa cells Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
Golgi apparatus
-
 Homo sapiens 5794
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ 1.9-fold increase in activity at Mn2+ concentrations between 2.5 and 10 mM Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
47000
-
 x * 54000, glycosylated FUT9, SDS-PAGE, x * 47000, deglycosylated FUT9, SDS-PAGE Homo sapiens
54000
-
 x * 54000, glycosylated FUT9, SDS-PAGE, x * 47000, deglycosylated FUT9, SDS-PAGE Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
GDP-beta-L-fucose + asialo-erythropoietin Homo sapiens wild-type FUT9 efficiently fucosylates di-, tri- and tetraantennary N-glycans from asialoEPO ?
-
 ?
additional information Homo sapiens the alpha3-fucosyltransferase IX, FUT9, catalyses the transfer of fucose in an alpha3 linkage onto terminal type II Galbeta4GlcNAc acceptors, the final step in the biosynthesis of the Lewisx epitope, in neurons, overview ?
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens Q5Q0U2
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein FUT9 is a type II membrane protein with three potential N-glycosylation sites Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
neuron
-
 Homo sapiens
-
NT2-N cell NTERA-2/cl.D1 cells are cultured and differentiated into NT2N neurons Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GDP-beta-L-fucose + asialo-erythropoietin wild-type FUT9 efficiently fucosylates di-, tri- and tetraantennary N-glycans from asialoEPO Homo sapiens ?
-
 ?
GDP-beta-L-fucose + GalNAcbeta1-4GlcNAcbeta1-3Galbeta1-4Glc activity of CEFT-2 Homo sapiens GDP + GalNAcbeta1-4(Fucalpha1-3)GlcNAcbeta1-3Galbeta1-4Glc
-
 ?
additional information the alpha3-fucosyltransferase IX, FUT9, catalyses the transfer of fucose in an alpha3 linkage onto terminal type II Galbeta4GlcNAc acceptors, the final step in the biosynthesis of the Lewisx epitope, in neurons, overview Homo sapiens ?
-
 ?

Subunits

Subunits Comment Organism
? x * 54000, glycosylated FUT9, SDS-PAGE, x * 47000, deglycosylated FUT9, SDS-PAGE Homo sapiens

Synonyms

Synonyms Comment Organism
alpha3-fucosyltransferase IX
-
 Homo sapiens
Fut9
-
 Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
 Homo sapiens