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Literature summary for 2.4.1.152 extracted from

  • Seelhorst, K.; Stacke, C.; Ziegelmueller, P.; Hahn, U.
    N-Glycosylations of human alpha1,3-fucosyltransferase IX are required for full enzyme activity (2013), Glycobiology, 23, 559-567.
    View publication on PubMed
Search for more literature for 2.4.1.152

Cloned(Commentary)

Cloned (Comment) Organism
applying a baculovirus system in Spodoptera frugiperda Sf9 cells Homo sapiens
expressed in Sf9 insect cells Homo sapiens

Protein Variants

Protein Variants Comment Organism
N101Q the mutant shows about 30% of wild type activity Homo sapiens
N101Q the mutant still shows about 27% of wild type activity Homo sapiens
N101Q/N153Q the mutations lead to an almost complete loss of enzymatic activity Homo sapiens
N153Q the mutant almost completely loses enzymatic activity Homo sapiens
N153Q the mutation leads to an almost complete loss of enzymatic activity Homo sapiens
N191Q/N153Q the mutant almost completely loses enzymatic activity Homo sapiens
N62Q the mutant shows about 60% of wild type activity Homo sapiens
N62Q the mutant still shows about 62% of wild type activity Homo sapiens
N62Q/N101Q the mutant almost completely loses enzymatic activity Homo sapiens
N62Q/N101Q the mutations lead to an almost complete loss of enzymatic activity Homo sapiens
N62Q/N153Q the mutant almost completely loses enzymatic activity Homo sapiens
N62Q/N153Q the mutations lead to an almost complete loss of enzymatic activity Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0026
-
 GDP-beta-L-fucose wild type enzyme, in citrate/phosphate buffer, pH 4.5, at 37°C Homo sapiens
0.0026
-
 GDP-beta-L-fucose recombinant wild type enzyme, at pH 4.5 and 37°C Homo sapiens
0.0047
-
 GDP-beta-L-fucose mutant enzyme N101Q, in citrate/phosphate buffer, pH 4.5, at 37°C Homo sapiens
0.0047
-
 GDP-beta-L-fucose mutant enzyme N101Q, at pH 4.5 and 37°C Homo sapiens
0.0061
-
 GDP-beta-L-fucose mutant enzyme N62Q, at pH 4.5 and 37°C Homo sapiens
0.0066
-
 GDP-beta-L-fucose mutant enzyme N62Q, in citrate/phosphate buffer, pH 4.5, at 37°C Homo sapiens
0.22
-
 N-acetyllactosamine mutant enzyme N101Q, in citrate/phosphate buffer, pH 4.5, at 37°C Homo sapiens
0.22
-
 N-acetyllactosamine mutant enzyme N101Q, at pH 4.5 and 37°C Homo sapiens
0.27
-
 N-acetyllactosamine mutant enzyme N62Q, in citrate/phosphate buffer, pH 4.5, at 37°C Homo sapiens
0.27
-
 N-acetyllactosamine mutant enzyme N62Q, at pH 4.5 and 37°C Homo sapiens
0.61
-
 N-acetyllactosamine wild type enzyme, in citrate/phosphate buffer, pH 4.5, at 37°C Homo sapiens
0.61
-
 N-acetyllactosamine recombinant wild type enzyme, at pH 4.5 and 37°C Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
41000
-
 x * 41000, fully glycosylated His-tagged enzyme, SDS-PAGE Homo sapiens
41000
-
 x * 41000, recombinant enzyme, SDS-PAGE Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
GDP-beta-L-fucose + N-acetyllactosamine Homo sapiens
-
 GDP + L-Fuc-alpha-(1->3)-[D-Gal-beta(1->4)]-D-GlcNac
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein the enzyme contains three putative N-glycosylation sites (Asn62, Asn101 and Asn153) Homo sapiens
glycoprotein the enzyme contains three potential N-glycosylation sites (Asn-XSer/Thr, X is any amino acid except proline) at amino acid positions 62, 101 and 153, respectively Homo sapiens

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA column chromatography Homo sapiens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.067
-
 mutant enzyme N101Q, at pH 4.5 and 37°C Homo sapiens
0.154
-
 mutant enzyme N62Q, at pH 4.5 and 37°C Homo sapiens
0.248
-
 wild type enzyme, at pH 4.5 and 37°C Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
GDP-beta-L-fucose + N-acetyllactosamine
-
 Homo sapiens GDP + ?
-
 ?
GDP-beta-L-fucose + N-acetyllactosamine
-
 Homo sapiens GDP + L-Fuc-alpha-(1->3)-[D-Gal-beta(1->4)]-D-GlcNac
-
 ?

Subunits

Subunits Comment Organism
? x * 41000, fully glycosylated His-tagged enzyme, SDS-PAGE Homo sapiens
? x * 41000, recombinant enzyme, SDS-PAGE Homo sapiens

Synonyms

Synonyms Comment Organism
alpha1,3-fucosyltransferase IX
-
 Homo sapiens
alpha1,3-FucTIX
-
 Homo sapiens
FucT IX
-
 Homo sapiens

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.042
-
 N-acetyllactosamine mutant enzyme N101Q, at pH 4.5 and 37°C C Homo sapiens
0.12
-
 N-acetyllactosamine mutant enzyme N62Q, at pH 4.5 and 37°C C Homo sapiens