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Literature summary for 2.4.1.155 extracted from

  • Khan, S.H.; Crawley, S.C.; Kanie, O.; Hindsgaul, O.
    A trisaccharide acceptor analog for N-acetylglucosaminyltransferase V which binds to the enzyme but sterically precludes the transfer reaction (1993), J. Biol. Chem., 268, 2468-2473.
    View publication on PubMed
Search for more literature for 2.4.1.155

Inhibitors

Inhibitors Comment Organism Structure
N-Acetyl-D-glucosaminyl-1,2-alpha-4-O-methyl-mannosyl-1,6-beta-D-glucosyl-O-(CH2)7-CH3
-
 Mesocricetus auratus
N-Acetyl-D-glucosaminyl-1,2-alpha-6-deoxy-mannosyl-1,6-beta-D-glucosyl-O-(CH2)7-CH3 kinetics Mesocricetus auratus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information kinetic study Mesocricetus auratus
0.023
-
 N-acetyl-D-glucosaminyl-1,2-alpha-mannosyl-1,6-beta-glucosyl-O-(CH2)7-CH3
-
 Mesocricetus auratus
0.036
-
 N-acetyl-D-glucosaminyl-1,2-alpha-mannosyl-1,6-beta-glucosyl-O-(CH2)7-CH3
-
 Mesocricetus auratus
0.074
-
 N-acetyl-D-glucosaminyl-1,2-alpha-4-deoxy-mannosyl-1,6-beta-D-glucosyl-O-(CH2)7-CH3
-
 Mesocricetus auratus
0.66 0.7 UDP-N-acetylgucosamine cosubstrate: N-acetyl-D-glucosaminyl-1,2-alpha-6-deoxy- (or-4-O-methyl-) mannosyl-1,6-beta-D-glucosyl-O-(CH2)7-CH3 Mesocricetus auratus
1.1 1.2 UDP-N-acetylglucosamine cosubstrate: N-acetyl-D-glucosaminyl-1,2-alpha-mannosyl-1,6-beta-glucosyl-O-(CH2)7-CH3 Mesocricetus auratus
1.4
-
 UDP-N-acetylgucosamine cosubstrate: N-acetyl-D-glucosaminyl-1,2-alpha-4-deoxy-mannosyl-1,6-beta-D-glucosyl-O-(CH2)7-CH3 Mesocricetus auratus

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
 Mesocricetus auratus 16020
-

Organism

Organism UniProt Comment Textmining
Mesocricetus auratus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial Mesocricetus auratus

Reaction

Reaction Comment Organism Reaction ID
UDP-N-acetyl-alpha-D-glucosamine + beta-D-GlcNAc-(1->2)-[beta-D-GlcNAc-(1->4)]-alpha-D-Man-(1->3)-[beta-D-GlcNAc-(1->2)-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc-N-Asn-[protein] = UDP + beta-D-GlcNAc-(1->2)-[beta-D-GlcNAc-(1->4)]-alpha-D-Man-(1->3)-[beta-D-GlcNAc-(1->2)-[beta-D-GlcNAc-(1->6)]-alpha-D-Man-(1->6)]-beta-D-Man-(1->4)-beta-D-GlcNAc-(1->4)-beta-D-GlcNAc-N-Asn-[protein] mechanism Mesocricetus auratus
a

Source Tissue

Source Tissue Comment Organism Textmining
kidney
-
 Mesocricetus auratus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDP-N-acetyl-D-glucosamine + N-acetyl-D-glucosaminyl-1,2-alpha-4-deoxy-mannosyl-1,6-beta-glucosyl-O-(CH2)7-CH3 good substrate Mesocricetus auratus UDP + N-acetyl-D-glucosaminyl-1,6-(N-acetyl-D-glucosaminyl-1,2)-alpha-4-deoxy-mannosyl-1,6-beta-D-mannosyl-O-(CH2)7-CH3
-
 ?
UDP-N-acetyl-D-glucosamine + N-acetyl-D-glucosaminyl-1,2-alpha-D-mannosyl-1,3(6)-(N-acetyl-D-glucosaminyl-1,2-alpha-D-mannosyl-1,6(3))-beta-D-mannosyl-1,4-N-acetyl-D-glucosaminyl-R
-
 Mesocricetus auratus UDP + N-acetyl-D-glucosaminyl-1,2-(N-acetyl-D-glucosaminyl-1,6)-1,2-alpha-D-mannosyl-1,3(6)-(N-acetyl-D-glucosaminyl-1,2-alpha-D-mannosyl-1,6(3))-beta-D-mannosyl-1,4-N-acetyl-D-glucosaminyl-R
-
 ?
UDP-N-acetyl-D-glucosamine + N-acetyl-D-glucosaminyl-1,2-alpha-mannosyl-1,6-beta-glucosyl-O-R best substrate Mesocricetus auratus UDP + N-acetyl-D-glucosaminyl-1,6-(N-acetyl-D-glucosaminyl-1,2)-alpha-mannosyl-1,6-beta-D-glucosyl-O-R
-
 ?
UDP-N-acetyl-D-glucosamine + N-acetyl-D-glucosaminyl-1,2-alpha-mannosyl-1,6-beta-glucosyl-O-R R: hydrophobic group, e.g. (CH2)7-CH3 Mesocricetus auratus UDP + N-acetyl-D-glucosaminyl-1,6-(N-acetyl-D-glucosaminyl-1,2)-alpha-mannosyl-1,6-beta-D-glucosyl-O-R
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
37
-
 assay at Mesocricetus auratus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.014
-
 N-Acetyl-D-glucosaminyl-1,2-alpha-4-O-methyl-mannosyl-1,6-beta-D-glucosyl-O-(CH2)7-CH3
-
 Mesocricetus auratus
0.063
-
 N-Acetyl-D-glucosaminyl-1,2-alpha-6-deoxy-mannosyl-1,6-beta-D-glucosyl-O-(CH2)7-CH3
-
 Mesocricetus auratus