Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | blockade of endocytosis, independent of Chs4p function, stops Chs3p internalization, triggering a significant increase in chitin synthesis, overview | Saccharomyces cerevisiae |
Cloned (Comment) | Organism |
---|---|
expression of Chs4p-GFP and Chs3p-GFP in CRM233 and CRM103 strains, respectively | Saccharomyces cerevisiae |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | the absence of Chs4p renders CSIII functionally inactive, independently of Chs3p accumulation at the plasma membrane | Saccharomyces cerevisiae |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
additional information | Chs4p is transported in vesicles in a polarized fashion independently of the other Chs proteins. Its association with membranes depends not only on prenylation, but also on its interaction with other proteins, mainly Chs3p, which is the catalytic subunit of CSIII and is able to properly direct Chs4p to the bud neck in the absence of prenylation | Saccharomyces cerevisiae | - |
- |
plasma membrane | chitin synthase III requires Chs4p-dependent translocation of Chs3p into the plasma membrane, Chs4p thus promotes Chs3p translocation into the plasma membrane in a stable and active form. In the absence of Chs4p, Chs3p is delivered to the plasma membrane but fails to accumulate there because it is rapidly endocytosed and accumulates in intracellular vesicles. The C-terminal region of Chs4p is required for its association with plasma membrane but not for biological function | Saccharomyces cerevisiae | 5886 | - |
transport vesicle | - |
Saccharomyces cerevisiae | 30133 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Saccharomyces cerevisiae | Chs4p is required for chitin synthase III activity and hence for chitin synthesis | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharomyces cerevisiae | P29465 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
side-chain modification | prenylation of CSIII components is involved in proper subcellular localization of the enzyme, overview | Saccharomyces cerevisiae |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cell culture | Chs3p and Chs4p appear to co-localize at the neck region of the budding yeast | Saccharomyces cerevisiae | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Chs4p is required for chitin synthase III activity and hence for chitin synthesis | Saccharomyces cerevisiae | ? | - |
? | |
additional information | proper CSIII turnover is maintained through the endocytic internalization of Chs3p, overview | Saccharomyces cerevisiae | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | the C-terminal region of Chs4p is required for its association with plasma membrane but not for biological function | Saccharomyces cerevisiae |
Synonyms | Comment | Organism |
---|---|---|
chitin synthase III | - |
Saccharomyces cerevisiae |
Chs3p | - |
Saccharomyces cerevisiae |
Chs4p | - |
Saccharomyces cerevisiae |
CSIII | - |
Saccharomyces cerevisiae |