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Literature summary for 2.4.1.16 extracted from

  • Reyes, A.; Sanz, M.; Duran, A.; Roncero, C.
    Chitin synthase III requires Chs4p-dependent translocation of Chs3p into the plasma membrane (2007), J. Cell Sci., 120, 1998-2009.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
additional information blockade of endocytosis, independent of Chs4p function, stops Chs3p internalization, triggering a significant increase in chitin synthesis, overview Saccharomyces cerevisiae

Cloned(Commentary)

Cloned (Comment) Organism
expression of Chs4p-GFP and Chs3p-GFP in CRM233 and CRM103 strains, respectively Saccharomyces cerevisiae

Inhibitors

Inhibitors Comment Organism Structure
additional information the absence of Chs4p renders CSIII functionally inactive, independently of Chs3p accumulation at the plasma membrane Saccharomyces cerevisiae

Localization

Localization Comment Organism GeneOntology No. Textmining
additional information Chs4p is transported in vesicles in a polarized fashion independently of the other Chs proteins. Its association with membranes depends not only on prenylation, but also on its interaction with other proteins, mainly Chs3p, which is the catalytic subunit of CSIII and is able to properly direct Chs4p to the bud neck in the absence of prenylation Saccharomyces cerevisiae
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plasma membrane chitin synthase III requires Chs4p-dependent translocation of Chs3p into the plasma membrane, Chs4p thus promotes Chs3p translocation into the plasma membrane in a stable and active form. In the absence of Chs4p, Chs3p is delivered to the plasma membrane but fails to accumulate there because it is rapidly endocytosed and accumulates in intracellular vesicles. The C-terminal region of Chs4p is required for its association with plasma membrane but not for biological function Saccharomyces cerevisiae 5886
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transport vesicle
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Saccharomyces cerevisiae 30133
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Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Saccharomyces cerevisiae Chs4p is required for chitin synthase III activity and hence for chitin synthesis ?
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?

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae P29465
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Posttranslational Modification

Posttranslational Modification Comment Organism
side-chain modification prenylation of CSIII components is involved in proper subcellular localization of the enzyme, overview Saccharomyces cerevisiae

Source Tissue

Source Tissue Comment Organism Textmining
cell culture Chs3p and Chs4p appear to co-localize at the neck region of the budding yeast Saccharomyces cerevisiae
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information Chs4p is required for chitin synthase III activity and hence for chitin synthesis Saccharomyces cerevisiae ?
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?
additional information proper CSIII turnover is maintained through the endocytic internalization of Chs3p, overview Saccharomyces cerevisiae ?
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?

Subunits

Subunits Comment Organism
More the C-terminal region of Chs4p is required for its association with plasma membrane but not for biological function Saccharomyces cerevisiae

Synonyms

Synonyms Comment Organism
chitin synthase III
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Saccharomyces cerevisiae
Chs3p
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Saccharomyces cerevisiae
Chs4p
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Saccharomyces cerevisiae
CSIII
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Saccharomyces cerevisiae