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Literature summary for 2.4.1.168 extracted from

  • Hayashi, T.; Matsuda, K.
    Biosynthesis of xyloglucan in suspension-cultured soybean cells. Occurrence and some properties of xyloglucan 4-beta-D-glucosyltransferase and 6-alpha-D-xylosyltransferase (1981), J. Biol. Chem., 256, 11117-11122.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
UDP-xylose activation, 0.01-0.03 mM Glycine max

General Stability

General Stability Organism
DTT, 1 mM, EDTA, 1 mM, sucrose, 0.4 M, bovine serum albumin, 0.1%, stabilize Glycine max

Inhibitors

Inhibitors Comment Organism Structure
GTPglucose weak Glycine max
additional information no inhibition by tunicamycin, ATP or GTP Glycine max
TDPglucose weak Glycine max
UTP
-
Glycine max

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
Glycine max 16020
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
UDP-D-glucose + xyloglucan Glycine max responsible for the formation of the xyloglucan backbone UDP + glucosylxyloglucan
-
?

Organism

Organism UniProt Comment Textmining
Glycine max
-
soy bean
-

Source Tissue

Source Tissue Comment Organism Textmining
cell suspension culture
-
Glycine max
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDP-D-glucose + (glucosyl)xyloglucan no acceptor: cello-oligosaccharides and fragment oligosaccharides from endoglucanase digest Glycine max UDP + (glucosyl-glucosyl)xyloglucan forms a beta-1,4-D-glucosyl-D-glucose linkage ?
UDP-D-glucose + (glucosyl)xyloglucan transfers a beta-D-glucosyl residue from UDPglucose on to a glucose residue in xyloglucan, other glucosyl-acceptors are beta-1,3-glucan and xylan Glycine max UDP + (glucosyl-glucosyl)xyloglucan forms a beta-1,4-D-glucosyl-D-glucose linkage ?
UDP-D-glucose + xyloglucan responsible for the formation of the xyloglucan backbone Glycine max UDP + glucosylxyloglucan
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
35
-
-
Glycine max

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5 7
-
Glycine max