Any feedback?
Literature summary for 2.4.1.17 extracted from
- The human UDP-glucuronosyltransferase: identification of key residues within the nucleotide-sugar binding site (2007), Mol. Pharmacol., 72, 604-611.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of wild-type and mutant isozymes as C-terminal Histagged fusion proteins in baculovirus-infected insect cells, expression levels, overview | Homo sapiens |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| H369A | site-directed mutagenesis of isozyme UGT1A9, the Km values for scopoletin and UDP-D-glucuronate are increased compared to the wild-type isozyme, the mutant shows impaired cosubstrate binding | Homo sapiens |
| H371A | site-directed mutagenesis of isozyme UGT1A6, the Km values for scopoletin and UDP-D-glucuronate are increased by 4 and 11fold, respectively, compared to the wild-type isozyme, the mutant shows severely impaired cosubstrate binding | Homo sapiens |
| H377A | site-directed mutagenesis of isozyme UGT1A9, inactive mutant | Homo sapiens |
| H379A | site-directed mutagenesis of isozyme UGT1A6, the Km values for scopoletin and UDP-D-glucuronate are increased compared to the wild-type isozyme, the mutant shows severely impaired cosubstrate binding | Homo sapiens |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | bisubstrate kinetics of wild-type and mutant isozyme UGT1A6 | Homo sapiens | |
| 0.048 | - |
1-naphthol | H379A mutant isozyme UGT1A6 | Homo sapiens |  |
| 0.08 | - |
scopoletin | wild-type isozyme UGT1A9 | Homo sapiens | |
| 0.083 | - |
1-naphthol | H371A mutant isozyme UGT1A6 | Homo sapiens | |
| 0.17 | - |
4-Methylumbelliferone | wild-type isozyme UGT1A6 | Homo sapiens | |
| 0.29 | - |
scopoletin | wild-type isozyme UGT1A6 | Homo sapiens | |
| 0.58 | - |
4-Methylumbelliferone | H379A mutant isozyme UGT1A6 | Homo sapiens | |
| 0.66 | - |
4-Methylumbelliferone | H371A mutant isozyme UGT1A6 | Homo sapiens | |
| 1.64 | - |
scopoletin | H369A mutant isozyme UGT1A9 | Homo sapiens | |
| 3.88 | - |
scopoletin | H371A mutant isozyme UGT1A6 | Homo sapiens | |
| 7.64 | - |
scopoletin | H379A mutant isozyme UGT1A6 | Homo sapiens | |
| 27 | - |
1-naphthol | wild-type isozyme UGT1A6 | Homo sapiens | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| endoplasmic reticulum membrane | - |
Homo sapiens | 5789 | - |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| UDP-D-glucuronate + acceptor | Homo sapiens | UDP-glucuronosyltransferases play important roles in the metabolism, detoxification,and clearance of many different xenobiotics, including drugs and endogenous compounds | UDP + acceptor beta-D-glucuronoside | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
isozyme UGT1A6 | - |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| UDP-alpha-D-glucuronate + acceptor = UDP + acceptor beta-D-glucuronoside | compulsory ordered bi bi mechanism | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| UDP-D-glucuronate + 1-naphthol | - |
Homo sapiens | UDP + 1-naphthyl beta-D-glucuronoside | - |
? | |
| UDP-D-glucuronate + 4-methylumbelliferone | - |
Homo sapiens | UDP + 4-methylumbelliferyl beta-D-glucuronoside | - |
? | |
| UDP-D-glucuronate + acceptor | - |
Homo sapiens | UDP + acceptor beta-D-glucuronoside | - |
? | |
| UDP-D-glucuronate + acceptor | UDP-glucuronosyltransferases play important roles in the metabolism, detoxification,and clearance of many different xenobiotics, including drugs and endogenous compounds | Homo sapiens | UDP + acceptor beta-D-glucuronoside | - |
? | |
| UDP-D-glucuronate + scopoletin | substrate binding structure, UGT1A6 residues His371 and Glu379 are directly involved in UDPGA binding but are not the general acid or general base, homology mocelling, overview | Homo sapiens | UDP + scopoletin beta-D-glucuronoside | - |
? | |
| UDP-D-glucuronate + umbelliferone | - |
Homo sapiens | UDP + umbelliferyl beta-D-glucuronoside | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| More | substrate binding structure, homology mnodelling of wild-type and mutant isozymes UGT1A6 and UGT1A9, overview | Homo sapiens |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| UDP-glucuronosyltransferase | - |
Homo sapiens |
| UGT | - |
Homo sapiens |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
