Application | Comment | Organism |
---|---|---|
medicine | some male athletes have testosterone to epitestosterone values greater than the accepted threshold value 4.0, even without testosterone abuse. The main reason for such false-positive results in doping tests is a low epitestosterone glucuronide concentration but not a high level of testosterone glucuronide | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
- |
Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0017 | - |
Epitestosterone | isoform UGT2B7, pH 7.4, 37°C | Homo sapiens | |
0.01 | - |
testosterone | isoform UGT2B17, pH 7.4, 37°C | Homo sapiens | |
0.0116 | - |
Epitestosterone | isoform UGT2A1, pH 7.4, 37°C | Homo sapiens | |
0.0387 | - |
testosterone | isoform UGT2A1, pH 7.4, 37°C | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
male athletes | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
epithelium | nasal epithelium, isoform UGT2A1 | Homo sapiens | - |
urine | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
epitestosterone + UDP-glucuronate | epitestosterone glucuronidation is catalyzed mainly by UGT2B7. No substrate for isoform UGT2B17, but it binds epitestosterone with affinity similar to that of testosterone. Isoform UGT2A1 catalyzes the glucuronidation of both steroids at considerable rates and similar kinetics | Homo sapiens | UDP + epitestosterone 17-O-glucuronide | - |
? | |
testosterone + UDP-glucuronate | isoform UGT2B17 is the most active enzyme in testosterone glucuronidation. Isoform UGT2A1, an extrahepatic enzyme that is expressed mainly in the nasal epithelium, catalyzes the glucuronidation of both steroids at considerable rates and similar kinetics | Homo sapiens | UDP + testosterone 17-O-glucuronide | - |
? |