Cloned (Comment) | Organism |
---|---|
gene UGT2B7, DNA and amino acid sequence determination and analysis, genotyping, three non-synonymous single nucleotide polymorphisms are identified, expression of CFP-tagged, YFP-tagged and HA-tagged UGT2B7 wild-type and mutant allozymes in Spodoptera frugiperda Sf9 cells via baculovirus transfection | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
A71S | naturally occuring mutation, non-synonymous single nucleotide polymorphism | Homo sapiens |
D398N | naturally occuring mutation, non-synonymous single nucleotide polymorphism | Homo sapiens |
H268Y | naturally occuring mutation, non-synonymous single nucleotide polymorphism | Homo sapiens |
additional information | protein-protein interactions among UGT2B7 allozymes wild-type, A71S, H268Y and D398N, by performing a systematic quantitative fluorescence resonance energy transfer (FRET) analysis in combination with co-immunoprecipitation assay, UGT2B7 allozymes form homo- and hetero-dimers and show distinct features in donor-acceptor distances. Both codon 71 and codon 268 in the N-terminal domain are involved in the dimeric interaction. Coimmunoprecipitation experiments also prove that UGT2B7 allozymes form stable dimers. An increase in activity is observed when wild-type hetero-dimerized with A71S compared with homo-dimers, while both H268Y and D398N impair the activity of WT and A71S by forming hetero-dimers. In addition, zidovudine glucuronidation activity is associated with FRET distance | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.508 | - |
zidovudine | pH 7.4, 37°C, recombinant HA-tagged wild-type dimer | Homo sapiens | |
0.519 | - |
zidovudine | pH 7.4, 37°C, recombinant CFP-tagged mutant H268Y dimer | Homo sapiens | |
0.534 | - |
zidovudine | pH 7.4, 37°C, recombinant HA-tagged mutant A71S dimer | Homo sapiens | |
0.54 | - |
zidovudine | pH 7.4, 37°C, recombinant dimer of CFP-tagged H268Y and HA-tagged D398N mutant monomers | Homo sapiens | |
0.57 | - |
zidovudine | pH 7.4, 37°C, recombinant HA-tagged mutant H268Y dimer | Homo sapiens | |
0.589 | - |
zidovudine | pH 7.4, 37°C, recombinant dimer of CFP-tagged A71S and HA-tagged D398N mutant monomers | Homo sapiens | |
0.605 | - |
zidovudine | pH 7.4, 37°C, recombinant CFP-tagged wild-type dimer | Homo sapiens | |
0.605 | - |
zidovudine | pH 7.4, 37°C, recombinant dimer of CFP-tagged wild-type and HA-tagged D398N mutant monomers | Homo sapiens | |
0.609 | - |
zidovudine | pH 7.4, 37°C, recombinant dimer of CFP-tagged A71S and HA-tagged H268Y mutant monomers | Homo sapiens | |
0.634 | - |
zidovudine | pH 7.4, 37°C, recombinant CFP-tagged mutant A71S dimer | Homo sapiens | |
0.706 | - |
zidovudine | pH 7.4, 37°C, recombinant dimer of CFP-tagged wild-type and HA-tagged A71S mutant monomers | Homo sapiens | |
0.841 | - |
zidovudine | pH 7.4, 37°C, recombinant dimer of CFP-tagged wild-type and HA-tagged H268Y mutant monomers | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Homo sapiens | 16020 | - |
microsome | - |
Homo sapiens | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-glucuronate + estriol | Homo sapiens | - |
UDP + estriol beta-D-glucuronoside | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P16662 | gene UGT2B7 | - |
Purification (Comment) | Organism |
---|---|
HA-tagged UGT2B7 wild-type and mutant allozymes in Spodoptera frugiperda Sf9 cells by hemagglutinin immunoaffinity chromatography | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-glucuronate + estriol | - |
Homo sapiens | UDP + estriol beta-D-glucuronoside | - |
? | |
UDP-glucuronate + zidovudine | - |
Homo sapiens | UDP + zidovudine O-beta-D-glucuronoside | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | UGT2B7 forms oligomers that affect its enzymatic activity. Proteinprotein interactions among UGT2B7 allozymes wild-type, A71S, H268Y and D398N, by performing a systematic quantitative fluorescence resonance energy transfer (FRET) analysis in combination with co-immunoprecipitation assay, UGT2B7 allozymes form homo- and hetero-dimers and show distinct features in donoracceptor distances. Both codon 71 and codon 268 in the N-terminal domain are involved in the dimeric interaction. Coimmunoprecipitation experiments also prove that UGT2B7 allozymes form stable dimers | Homo sapiens |
Synonyms | Comment | Organism |
---|---|---|
UDP-glucuronosyltransferase 2B7 | - |
Homo sapiens |
UGT2B7 | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Homo sapiens |
General Information | Comment | Organism |
---|---|---|
physiological function | UGT2B7 is one of the most important UGTs that glucuronidates abundant endobiotics and xenobiotics, such as estriol, morphine, and anticancer drugs | Homo sapiens |