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Literature summary for 2.4.1.170 extracted from

  • Funaki, A.; Waki, T.; Noguchi, A.; Kawai, Y.; Yamashita, S.; Takahashi, S.; Nakayama, T.
    Identification of a highly specific isoflavone 7-O-glucosyltransferase in the soybean (Glycine max (L.) Merr.) (2015), Plant Cell Physiol., 56, 1512-1520.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
gene GmUGT1, DNA and amino acid sequence determination and analysis, phylogenetic analysis, genotyping, quantitative real-time RT-PCR enzyme expression analysis Glycine max
gene GmUGT3, DNA and amino acid sequence determination and analysis, phylogenetic analysis, genotyping, quantitative real-time RT-PCR enzyme expression analysis Glycine max
gene GmUGT4, DNA and amino acid sequence determination and analysis, phylogenetic analysis, genotyping, quantitative real-time RT-PCR enzyme expression analysis Glycine max
gene GmUGT7, DNA and amino acid sequence determination and analysis, phylogenetic analysis, genotyping, quantitative real-time RT-PCR enzyme expression analysis Glycine max
gene GmUGT9, DNA and amino acid sequence determination and analysis, phylogenetic analysis, genotyping, quantitative real-time RT-PCR enzyme expression analysis Glycine max

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00031
-
genistein pH 8.5, 30°C, recombinant isozyme GmUGT3 Glycine max
0.00042
-
daidzein pH 8.5, 30°C, recombinant isozyme GmUGT3 Glycine max
0.0103
-
daidzein pH 8.5, 30°C, recombinant isozyme GmUGT3 Glycine max
0.0129
-
genistein pH 8.5, 30°C, recombinant isozyme GmUGT3 Glycine max
0.017
-
genistein pH 8.5, 30°C, recombinant isozyme GmUGT1 Glycine max
0.0189
-
daidzein pH 8.5, 30°C, recombinant isozyme GmUGT1 Glycine max
0.0203
-
daidzein pH 8.5, 30°C, recombinant isozyme GmUGT3 Glycine max
0.0237
-
genistein pH 8.5, 30°C, recombinant isozyme GmUGT3 Glycine max
0.0267
-
genistein pH 8.5, 30°C, recombinant isozyme GmUGT3 Glycine max
0.049
-
UDP-glucose pH 8.5, 30°C, recombinant isozyme GmUGT3 Glycine max
0.0537
-
UDP-glucose pH 8.5, 30°C, recombinant isozyme GmUGT1 Glycine max
0.0564
-
UDP-glucose pH 8.5, 30°C, recombinant isozyme GmUGT3 Glycine max
0.0869
-
UDP-glucose pH 8.5, 30°C, recombinant isozyme GmUGT3 Glycine max
0.0985
-
daidzein pH 8.5, 30°C, recombinant isozyme GmUGT3 Glycine max
0.1637
-
UDP-glucose pH 8.5, 30°C, recombinant isozyme GmUGT3 Glycine max

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
UDP-glucose + daidzein Glycine max
-
UDP + daidzein 7-O-beta-D-glucoside i.e. daidzin ?
UDP-glucose + genistein Glycine max
-
UDP + genistein 7-O-beta-D-glucoside i.e. genistin ?

Organism

Organism UniProt Comment Textmining
Glycine max
-
gene GmUGT3
-
Glycine max
-
gene GmUGT4
-
Glycine max
-
gene GmUGT7
-
Glycine max
-
gene GmUGT9
-
Glycine max A6BM07 gene GmUGT1
-

Source Tissue

Source Tissue Comment Organism Textmining
cotyledon
-
Glycine max
-
hypocotyl
-
Glycine max
-
additional information gene GmUGT3 encodes a UGT that is highly specific for isoflavones Glycine max
-
additional information gene GmUGT4 encodes a UGT that is highly specific for isoflavones and is mainly expressed in the lateral roots and seeds, followed by taproots, cotyledon, and hypocotyls Glycine max
-
additional information gene GmUGT9 encodes a UGT that is highly specific for isoflavones, the levels of the GmUGT9 transcripts are highest in roots, followed by taproots, cotyledon, and hypocotyls Glycine max
-
additional information the levels of the most abundant GmUGT1 transcripts in cotyledons are much higher than those in lateral roots Glycine max
-
additional information the levels of the most abundant GmUGT7 transcripts in cotyledons are much higher than those in lateral roots Glycine max
-
root lateral Glycine max
-
root lateral and taproot Glycine max
-
seed
-
Glycine max
-
seedling
-
Glycine max
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
GmUGT1 activity with other flavonoids, overview Glycine max
0.045
-
substrate genistein, pH 8.5, 30°C, purified recombinant enzyme Glycine max
0.049
-
substrate daidzein, pH 8.5, 30°C, purified recombinant enzyme Glycine max
0.158
-
substrate genistein, pH 8.5, 30°C, purified recombinant enzyme Glycine max
0.209
-
substrate genistein, pH 8.5, 30°C, purified recombinant enzyme Glycine max
0.253
-
substrate daidzein, pH 8.5, 30°C, purified recombinant enzyme Glycine max
0.253
-
substrate genistein, pH 8.5, 30°C, purified recombinant enzyme Glycine max
0.457
-
substrate daidzein, pH 8.5, 30°C, purified recombinant enzyme Glycine max
0.465
-
substrate genistein, pH 8.5, 30°C, purified recombinant enzyme Glycine max
0.467
-
substrate daidzein, , pH 8.5, 30°C, purified recombinant enzyme Glycine max

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information GmUGT1 is active with UDP-galactose and UDP-glucuronic acid as substrates. It displays high specific activities toward isoflavones (genistein and daidzein) to produce their 7-O-beta-D-glucosides, it also shows considerable levels of activities toward other flavonoids such as kaempferol, quercetin, naringenin and apigenin. GmUGT1 is also capable of acting on the flavone luteolin, (S)-equol, a 4',7-isoflavandiol, and (R)-equol Glycine max ?
-
?
additional information in contrast to GmUGT1, UDP-galactose and UDP-glucuronic acid are essentially inert as substrates for GmUGT3, glucosyl-acceptor substrate specificity, overview. No activity with kaempferol, quercetin, naringenin, apigenin, and luteolin Glycine max ?
-
?
additional information in contrast to GmUGT1, UDP-galactose and UDP-glucuronic acid are essentially inert as substrates for GmUGT4, glucosyl-acceptor substrate specificity, overview. No activity with kaempferol, quercetin, naringenin, apigenin, and luteolin Glycine max ?
-
?
UDP-glucose + daidzein
-
Glycine max UDP + daidzein 7-O-beta-D-glucoside i.e. daidzin ?
UDP-glucose + genistein
-
Glycine max UDP + genistein 7-O-beta-D-glucoside i.e. genistin ?

Synonyms

Synonyms Comment Organism
GmUGT1
-
Glycine max
GmUGT3
-
Glycine max
GmUGT4
-
Glycine max
GmUGT7
-
Glycine max
GmUGT9
-
Glycine max

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Glycine max

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.1
-
daidzein pH 8.5, 30°C, recombinant isozyme GmUGT3 Glycine max
0.11
-
UDP-glucose pH 8.5, 30°C, recombinant isozyme GmUGT3 Glycine max
0.13
-
genistein pH 8.5, 30°C, recombinant isozyme GmUGT3 Glycine max
0.39
-
genistein pH 8.5, 30°C, recombinant isozyme GmUGT3 Glycine max
0.97
-
daidzein pH 8.5, 30°C, recombinant isozyme GmUGT3 Glycine max
1.22
-
daidzein pH 8.5, 30°C, recombinant isozyme GmUGT3 Glycine max
1.41
-
UDP-glucose pH 8.5, 30°C, recombinant isozyme GmUGT3 Glycine max
1.72
-
UDP-glucose pH 8.5, 30°C, recombinant isozyme GmUGT3 Glycine max
1.75
-
genistein pH 8.5, 30°C, recombinant isozyme GmUGT3 Glycine max
4.34
-
genistein pH 8.5, 30°C, recombinant isozyme GmUGT1 Glycine max
5.75
-
daidzein pH 8.5, 30°C, recombinant isozyme GmUGT1 Glycine max
5.89
-
daidzein pH 8.5, 30°C, recombinant isozyme GmUGT3 Glycine max
6.04
-
genistein pH 8.5, 30°C, recombinant isozyme GmUGT3 Glycine max
7.65
-
UDP-glucose pH 8.5, 30°C, recombinant isozyme GmUGT3 Glycine max
8.79
-
UDP-glucose pH 8.5, 30°C, recombinant isozyme GmUGT1 Glycine max

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
assay at Glycine max

General Information

General Information Comment Organism
evolution nine different GmUGT cDNAs that are related to GmUGT1 are obtained, some of which are classified into two phylogenetically different subgroups, phylogenetic analysis, overview. The enzymes of subgroup A, GmUGT3, GmUGT4, and GmUGT9, all are highly specific for isoflavones. Gene GmUGT4, a representative of subgroup A, encodes a UGT that is highly specific for isoflavones and is mainly expressed in the lateral roots and seeds, while gene GmUGT1, a representative of subgroup B, encodes an enzyme displaying a broad glucosyl-acceptor specificity and is expressed in the aerial parts, i.e. cotyledons, hypocotyls and pods Glycine max
evolution nine different GmUGT cDNAs that are related to GmUGT1, UniProt ID A6BM07, are obtained, some of which are classified into two phylogenetically different subgroups, phylogenetic analysis, overview Glycine max
evolution nine different GmUGT cDNAs that are related to GmUGT1, UniProt ID A6BM07, are obtained, some of which are classified into two phylogenetically different subgroups, phylogenetic analysis, overview. The enzymes of subgroup A, GmUGT3, GmUGT4, and GmUGT9, all are highly specific for isoflavones Glycine max
evolution nine different GmUGT cDNAs that are related to GmUGT1, UniProt ID A6BM07, are obtained, some of which are classified into two phylogenetically different subgroups, phylogenetic analysis, overview. The enzymes of subgroup A, GmUGT3, GmUGT4, and GmUGT9, all are highly specific for isoflavones. Gene GmUGT4, a representative of subgroup A, encodes a UGT that is highly specific for isoflavones and is mainly expressed in the lateral roots and seeds, while gene GmUGT1, a representative of subgroup B, encodes an enzyme displaying a broad glucosyl-acceptor specificity and is expressed in the aerial parts, i.e. cotyledons, hypocotyls and pods Glycine max
metabolism the isoflavone 7-O-glucosyltransferase is involved in isoflavone conjugate biosynthesis Glycine max
physiological function the isoflavone 7-O-glucosyltransferase is involved in isoflavone conjugate biosynthesis. Isoflavone conjugates, 7-O-beta-D-glucosides and 7-O-(6''-malonyl-beta-D-glucosides) of daidzein and genistein, accumulate in soybean roots and serve as the stored precursors of isoflavones (aglycons), which play very important roles in the rhizobia-mediated nodulation of this plant Glycine max
physiological function the isoflavone 7-O-glucosyltransferase is involved in isoflavone conjugate biosynthesis. Isoflavone conjugates, 7-O-beta-D-glucosides and 7-O-(6''-malonyl-beta-D-glucosides) of daidzein and genistein, accumulate in soybean roots and serve as the stored precursors of isoflavones (aglycons), which play very important roles in the rhizobia-mediated nodulation of this plant. Isozyme GmUGT4, but not GmUGT1, plays an exclusive role in the conjugation of isoflavones in the lateral roots of the soybean plant Glycine max

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.25
-
UDP-glucose pH 8.5, 30°C, recombinant isozyme GmUGT3 Glycine max
2
-
UDP-glucose pH 8.5, 30°C, recombinant isozyme GmUGT3 Glycine max
10.5
-
UDP-glucose pH 8.5, 30°C, recombinant isozyme GmUGT3 Glycine max
88
-
UDP-glucose pH 8.5, 30°C, recombinant isozyme GmUGT3 Glycine max
94.8
-
daidzein pH 8.5, 30°C, recombinant isozyme GmUGT3 Glycine max
124
-
daidzein pH 8.5, 30°C, recombinant isozyme GmUGT3 Glycine max
136
-
genistein pH 8.5, 30°C, recombinant isozyme GmUGT3 Glycine max
164
-
UDP-glucose pH 8.5, 30°C, recombinant isozyme GmUGT1 Glycine max
167
-
genistein pH 8.5, 30°C, recombinant isozyme GmUGT3 Glycine max
226
-
genistein pH 8.5, 30°C, recombinant isozyme GmUGT3 Glycine max
244
-
daidzein pH 8.5, 30°C, recombinant isozyme GmUGT3 Glycine max
255
-
genistein pH 8.5, 30°C, recombinant isozyme GmUGT1 Glycine max
291
-
daidzein pH 8.5, 30°C, recombinant isozyme GmUGT3 Glycine max
304
-
daidzein pH 8.5, 30°C, recombinant isozyme GmUGT1 Glycine max
409
-
genistein pH 8.5, 30°C, recombinant isozyme GmUGT3 Glycine max