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Literature summary for 2.4.1.173 extracted from

  • Kalinowska, M.; Wojciechowski, Z.A.
    Modulation of activities of steryl glucoside hydrolase and UDPG:sterol glucosyltransferase from Sinapis alba by detergents and lipids (1986), Phytochemistry, 25, 45-49.
No PubMed abstract available
Search for more literature for 2.4.1.173

Activating Compound

Activating Compound Comment Organism Structure
Phospholipids not stimulatory Sinapis alba
Triton X-100 activity stimulated by 0.1-1% with 6.5fold stimulation at 0.1%, highest activity stimulation with respect to others non-ionic detergents Sinapis alba
Tween-20 2fold activity stimulation at 0.1% Sinapis alba
Tween-40 70% activity stimulation at 0.05% Sinapis alba
Tween-60 35% activity stimulation at 0.1% Sinapis alba

Inhibitors

Inhibitors Comment Organism Structure
sodium deoxycholate strong inhibition at 0.1-1% Sinapis alba
sodium taurocholate strong inhibition at 0.1-1% Sinapis alba

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane organelles membrane Sinapis alba 16020
-

Organism

Organism UniProt Comment Textmining
Sinapis alba
-
 white mustard
-

Purification (Commentary)

Purification (Comment) Organism
partial purification by differential centrifugation and precipitation with Me2CO Sinapis alba

Source Tissue

Source Tissue Comment Organism Textmining
seedling
-
 Sinapis alba
-

Storage Stability

Storage Stability Organism
-20 C, Tris-HCl 0.1 M, pH 7.2, two weeks Sinapis alba

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
UDP-glucose + cholesterol
-
 Sinapis alba UDP + cholesterol 3-beta-D-glucoside
-
 ?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Sinapis alba

pH Stability

pH Stability pH Stability Maximum Comment Organism
7.2
-
 Tris-HCl 0.1 M Sinapis alba