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Literature summary for 2.4.1.173 extracted from

  • Yoshikawa, T.; Furuya, T.
    Purification and properties of sterol:UDPG glucosyltransferase in cell culture of Digitalis purpurea (1979), Phytochemistry, 18, 239-241.
No PubMed abstract available

Activating Compound

Activating Compound Comment Organism Structure
2-mercaptoethanol activated by Digitalis purpurea
DIECA activated by Digitalis purpurea
EDTA activated by Digitalis purpurea

Inhibitors

Inhibitors Comment Organism Structure
NH4+ inhibited by Digitalis purpurea

Localization

Localization Comment Organism GeneOntology No. Textmining
microsome
-
Digitalis purpurea
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ inhibited by Digitalis purpurea
Zn2+ inhibited by Digitalis purpurea

Organism

Organism UniProt Comment Textmining
Digitalis purpurea
-
-
-

Purification (Commentary)

Purification (Comment) Organism
partial purification by differential centrifugation and Sephadex G-100 column, DEAE-Sephadex A-50 and Sepharose6B Digitalis purpurea

Source Tissue

Source Tissue Comment Organism Textmining
cell culture leaf cell culture Digitalis purpurea
-
leaf crude extracts have 2-5 times lower activity than cell cultures Digitalis purpurea
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information almost no activity with androsterone, digitoxigenin, digitoxin and testosterone Digitalis purpurea ?
-
?
UDP-glucose + cholesterol best substrate Digitalis purpurea UDP + cholesterol 3-beta-D-glucoside
-
?
UDP-glucose + epiandrosterone best substrate Digitalis purpurea UDP + epiandrosterone 3-beta-glucoside
-
?
UDP-glucose + pregnenolone 57% of the activity with epiandrosterone Digitalis purpurea UDP + pregnenolone 3-beta-glucoside
-
?
UDP-glucose + sitosterol 88% of the activity with epiandrosterone Digitalis purpurea UDP + sitosterol 3-beta-D-glucoside
-
?
UDP-glucose + stigmasterol 122% of the activity with epiandrosterone, highest specificity Digitalis purpurea UDP + stigmasterol 3-beta-D-glucoside
-
?

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Digitalis purpurea

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
-
Digitalis purpurea

pH Stability

pH Stability pH Stability Maximum Comment Organism
7.5
-
Tris-HCl 0.1 M, EDTA 10 mM, 2-mercaptoethanol 1 mM, sodium-deoxycholate 0.1% Digitalis purpurea