Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Arabidopsis thaliana |
expression in Escherichia coli | Avena sativa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
66000 | - |
cloned, N-terminal peptide of 133 amino acid could be cleaved resulting in a molecular weight of 52000 in agreement with 56000 of the purified enzyme | Avena sativa |
69000 | - |
cloned, calculated molecular mass | Arabidopsis thaliana |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | Q9M8Z7 | ugt80A2 | - |
Avena sativa | O22678 | ugt80A1 | - |
Avena sativa ugt80A1 | O22678 | ugt80A1 | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
flower | cDNA library | Arabidopsis thaliana | - |
leaf | cDNA library | Arabidopsis thaliana | - |
root | cDNA library | Arabidopsis thaliana | - |
shoot | cDNA library | Arabidopsis thaliana | - |
shoot | cloned from | Avena sativa | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-glucose + cholesterol | cloned enzyme exhibits equal activity to the purified protein | Arabidopsis thaliana | UDP + cholesterol 3-beta-D-glucoside | - |
? | |
UDP-glucose + cholesterol | cloned enzyme exhibits equal activity to the purified protein | Avena sativa | UDP + cholesterol 3-beta-D-glucoside | - |
? | |
UDP-glucose + cholesterol | cloned enzyme exhibits equal activity to the purified protein | Avena sativa ugt80A1 | UDP + cholesterol 3-beta-D-glucoside | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Arabidopsis thaliana |
30 | - |
assay at | Avena sativa |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Arabidopsis thaliana |
8 | - |
Tris-HCl 100 mM, dithiothreitol 1 mM, Triton X-100 0.2% | Avena sativa |