Activating Compound | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | - |
Solanum melongena | |
additional information | stimulated by acylglycerols containing unsaturated fatty acid residues | Solanum melongena | |
Triton X-100 | 130fold at 0.1% | Solanum melongena |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-Hydroxy-5-nitrobenzyl bromide | - |
Solanum melongena | |
4,4'-diisothiocyanatostilbene-2,2'-disulfonic acid | - |
Solanum melongena | |
4-acetamido-4'-isothiocyanatostilbene-2,2'-disulfonic acid | - |
Solanum melongena | |
cholesteryl glucoside | - |
Solanum melongena | |
Co2+ | - |
Solanum melongena | |
diacylglycerol | with saturated fatty acids | Solanum melongena | |
diethyldicarbonate | protected against inactivation upon pre-incubation with stigmast-5-en-3beta-ol or UDP-glucose | Solanum melongena | |
monoacylglycerol | with saturated fatty acids | Solanum melongena | |
additional information | inhibited at high ionic strength, 50% inhibition at 0.52 M NaF or 0.29 M NaCl; inhibited by dicarboxylic amino acids-modifying reagent Woodwards Reagent K protected against inactivation upon pre-incubation with UDP-glucose but not with stigmast-5-en-3beta-ol; no effect with Mg2+, Ca2+, Mn2+, Tween20, Tween 40, Tween 60, Tween 80, Tyloxapol, sodium deoxycholate, EDTA, EGTA, and reagents modifying serine, arginine and tyrosine side chains | Solanum melongena | |
N-ethylmaleimide | protected against inactivation upon pre-incubation with stigmast-5-en-3beta-ol or UDP-glucose | Solanum melongena | |
n-octyl thioglucoside | irreversible inhibited, 50% of activity at 4.8 mM | Solanum melongena | |
p-chloromercuribenzenesulfonic acid | - |
Solanum melongena | |
pyridoxal 5'-phosphate | - |
Solanum melongena | |
triacylglycerol | with saturated fatty acids | Solanum melongena | |
UDP | - |
Solanum melongena | |
UTP | - |
Solanum melongena | |
Zn2+ | - |
Solanum melongena |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00143 | - |
stigmast-5-en-3beta-ol | pH 7,3, 30°C | Solanum melongena | |
0.01429 | - |
UDP-glucose | pH 7.3, 30°C | Solanum melongena |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | membrane-bound | Solanum melongena | 16020 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Solanum melongena | may participate in the regulating of free sterol concentration in the cell | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Solanum melongena | - |
cv Black Beauty, eggplant | - |
Purification (Comment) | Organism |
---|---|
partial | Solanum melongena |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Solanum melongena | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(22S,25S)-5alpha-solanidanin-3beta-ol + UDP-glucose | demissidine 1.4% of the activity with sitosterol (stigmast-5-en-3beta-ol) | Solanum melongena | ? | - |
? | |
(22S,25S)-epoxy-furost-5-en-3beta,26-diol + UDP-glucose | nuatigenin, 16.1% of the activity with sitosterol (stigmast-5-en-3beta-ol) | Solanum melongena | ? | - |
? | |
(25R)-5alpha-spirostan-3beta-ol + UDP-glucose | tigogenin, 2.1% of the activity with sitosterol (stigmast-5-en-3beta-ol) | Solanum melongena | ? | - |
? | |
(25R)-5alpha-spirostan-3beta-ol-12-one + UDP-glucose | hecogenin, 6.3% of the activity with sitosterol (stigmast-5-en-3beta-ol) | Solanum melongena | ? | - |
? | |
(25R)-spirost-5-en-3beta,25beta-diol + UDP-glucose | isonuatigenin, 13.4% of the activity with sitosterol (stigmast-5-en-3beta-ol) | Solanum melongena | ? | - |
? | |
(25R)-spirost-5-en-3beta-ol + UDP-glucose | diosgenin, 3.5% of the activity with sitosterol (stigmast-5-en-3beta-ol) | Solanum melongena | ? | - |
? | |
(25S)-5alpha,22betaN-spirosolanin-3beta-ol + UDP-glucose | tomatidine, 2.8% of the activity with sitosterol (stigmast-5-en-3beta-ol) | Solanum melongena | ? | - |
? | |
5alpha-cholest-7-en-3beta-ol + UDP-glucose | 14.1% of the activity with sitosterol (stigmast-5-en-3beta-ol) | Solanum melongena | ? | - |
? | |
5alpha-cholestan-3beta-ol + UDP-glucose | cholestanol, 12.0% of the activity with sitosterol (stigmast-5-en-3beta-ol) | Solanum melongena | ? | - |
? | |
androst-5-en-3beta-ol-17-one + UDP-glucose | androstenolon, 16.0% of the activity with sitosterol (stigmast-5-en-3beta-ol) | Solanum melongena | ? | - |
? | |
cholest-5-en-22-on-3beta-ol + UDP-glucose | 22-oxycholesterol, 133.1% of the activity with sitosterol (stigmast-5-en-3beta-ol) | Solanum melongena | ? | - |
? | |
cholest-5-en-3beta,19-diol + UDP-glucose | 9.2% of the activity with sitosterol (stigmast-5-en-3beta-ol) | Solanum melongena | ? | - |
? | |
cholest-5-en-3beta,20alpha-diol + UDP-glucose | 90.8% of the activity with sitosterol (stigmast-5-en-3beta-ol) | Solanum melongena | ? | - |
? | |
cholest-5-en-3beta,25-diol + UDP-glucose | 25-hydroxycholesterol, 89.4% of the activity with sitosterol (stigmast-5-en-3beta-ol) | Solanum melongena | ? | - |
? | |
cholest-5-en-3beta-ol + UDP-glucose | cholesterol, 98.5% of the activity with sitosterol (stigmast-5-en-3beta-ol) | Solanum melongena | ? | - |
? | |
cholest-5-en-3beta-ol-7-one + UDP-glucose | 5.6% of the activity with sitosterol (stigmast-5-en-3beta-ol) | Solanum melongena | ? | - |
? | |
additional information | may participate in the regulating of free sterol concentration in the cell | Solanum melongena | ? | - |
? | |
additional information | UDP-galactose, TDP-glucose and CDP-glucose are also suitable as a sugar source for sterol glucosylation but with distinctly lower rates | Solanum melongena | ? | - |
? | |
additional information | no activity with solasodine (25R-22alphaN-spirosol-5-enin-3beta-ol), solanidine (22S,25S-solanid-5-enin-3beta-ol), sarsasapogenin (25S-5beta-spirostan-3beta-ol), yamogenin (25S-spirost-5-en-3beta-ol), thiocholesterol (cholest-5-en-3beta-tiol), 5beta-cholestan-3beta-ol (coprostanol), 5alpha-cholestan-3alpha-ol (epicholestanol), 5beta-cholestan-3alpha-ol (epicoprostanol), lanosterol (4,4,14-trimethyl-5alpha-cholest-8-en-3beta-ol), and beta-amyrin (olean-12-en-3beta-ol) do not serve as glucose acceptors | Solanum melongena | ? | - |
? | |
pregn-5-en-3beta-ol-20-one + UDP-glucose | pregnenolon, 33.1% of the activity with sitosterol (stigmast-5-en-3beta-ol) | Solanum melongena | ? | - |
? | |
stigmast-5-en-3beta-ol + UDP-glucose | sitosterol, 100% of the activity | Solanum melongena | additional information | 3-O-beta-D-monoglucopyranoside of sitosterol and 3-O-beta-D-monogalactopyranoside of sitosterol identified as products | ? | |
stigmasta-5,22-dien-3beta-ol + UDP-glucose | stigmasterol, 49.2% of the activity with sitosterol (stigmast-5-en-3beta-ol) | Solanum melongena | ? | - |
? | |
stigmasta-5,24(28)-dien-3beta-ol + UDP-glucose | 82.4% of the activity with sitosterol (stigmast-5-en-3beta-ol) | Solanum melongena | ? | - |
? | |
stigmastan-3beta-ol + UDP-glucose | 38.7% of the activity with sitosterol (stigmast-5-en-3beta-ol) | Solanum melongena | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
UDP-glucose:sterol glucosyltransferase | - |
Solanum melongena |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.035 | - |
pH 7.3, 30°C | Solanum melongena | UTP | |
0.065 | - |
pH 7.3, 30°C | Solanum melongena | UDP | |
0.09 | - |
pH 7.3, 30°C | Solanum melongena | Zn2+ | |
0.53 | - |
pH 7.3, 30°C | Solanum melongena | Co2+ |