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Literature summary for 2.4.1.173 extracted from
- Sterol 3beta-glucosyltransferase biocatalysts with a range of selectivities, including selectivity for testosterone (2013), Mol. Biosyst., 9, 2816-2822.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| gene ATG26, DNA and amino acid sequence determination and analysis, recombinant expression of N-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3) | Komagataella pastoris |
| gene ATG26, DNA and amino acid sequence determination and analysis, recombinant expression of N-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3) | Saccharomyces cerevisiae |
| gene ATG26, DNA and amino acid sequence determination and analysis, recombinant expression of N-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3) | Ogataea angusta |
| gene ATG26, DNA and amino acid sequence determination and analysis, recombinant expression of N-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3) | Kluyveromyces lactis |
| gene ATG26, DNA and amino acid sequence determination and analysis, recombinant expression of N-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3) | Debaryomyces hansenii |
| gene PGUG_04770, DNA and amino acid sequence determination and analysis, recombinant expression of N-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3) | Meyerozyma guilliermondii |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.0659 | - |
cholesterol | pH 7.8, 30°C | Kluyveromyces lactis |  |
| 0.0672 | - |
cholesterol | pH 7.8, 30°C | Ogataea angusta | |
| 0.8379 | - |
testosterone | pH 7.8, 30°C | Ogataea angusta | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mn2+ | required | Komagataella pastoris | |
| Mn2+ | required | Saccharomyces cerevisiae | |
| Mn2+ | required | Meyerozyma guilliermondii | |
| Mn2+ | required | Ogataea angusta | |
| Mn2+ | required | Kluyveromyces lactis | |
| Mn2+ | required | Debaryomyces hansenii | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 54200 | - |
x * 54200, about, sequence calculation | Ogataea angusta |
| 55900 | - |
x * 55900, about, sequence calculation | Kluyveromyces lactis |
| 61800 | - |
x * 61800, about, sequence calculation | Debaryomyces hansenii |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Debaryomyces hansenii | Q6BN88 | gene ATG26 | - |
| Debaryomyces hansenii CBS 767 | Q6BN88 | gene ATG26 | - |
| Kluyveromyces lactis | Q6CUV2 | gene ATG26 | - |
| Kluyveromyces lactis DSM 70799 | Q6CUV2 | gene ATG26 | - |
| Komagataella pastoris | Q9Y751 | gene ATG26 | - |
| Komagataella pastoris GS115 | Q9Y751 | gene ATG26 | - |
| Meyerozyma guilliermondii | A5DNB9 | gene PGUG_04770 | - |
| Meyerozyma guilliermondii DSM 6381 | A5DNB9 | gene PGUG_04770 | - |
| Ogataea angusta | A7KAK6 | gene ATG26 | - |
| Ogataea angusta DSM 70277 | A7KAK6 | gene ATG26 | - |
| Saccharomyces cerevisiae | Q06321 | gene ATG26 | - |
| Saccharomyces cerevisiae ATCC 204508 | Q06321 | gene ATG26 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant N-terminally His6-tagged enzyme from Escherichia coli strain BL21(DE3) | Komagataella pastoris |
| recombinant N-terminally His6-tagged enzyme from Escherichia coli strain BL21(DE3) | Saccharomyces cerevisiae |
| recombinant N-terminally His6-tagged enzyme from Escherichia coli strain BL21(DE3) | Meyerozyma guilliermondii |
| recombinant N-terminally His6-tagged enzyme from Escherichia coli strain BL21(DE3) | Ogataea angusta |
| recombinant N-terminally His6-tagged enzyme from Escherichia coli strain BL21(DE3) | Kluyveromyces lactis |
| recombinant N-terminally His6-tagged enzyme from Escherichia coli strain BL21(DE3) | Debaryomyces hansenii |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| UDP-alpha-D-glucose + testosterone | - |
Komagataella pastoris | UDP + testosterone 17-beta-D-glucoside | - |
? | |
| UDP-alpha-D-glucose + testosterone | - |
Saccharomyces cerevisiae | UDP + testosterone 17-beta-D-glucoside | - |
? | |
| UDP-alpha-D-glucose + testosterone | - |
Meyerozyma guilliermondii | UDP + testosterone 17-beta-D-glucoside | - |
? | |
| UDP-alpha-D-glucose + testosterone | - |
Ogataea angusta | UDP + testosterone 17-beta-D-glucoside | - |
? | |
| UDP-alpha-D-glucose + testosterone | - |
Kluyveromyces lactis | UDP + testosterone 17-beta-D-glucoside | - |
? | |
| UDP-alpha-D-glucose + testosterone | - |
Debaryomyces hansenii | UDP + testosterone 17-beta-D-glucoside | - |
? | |
| UDP-alpha-D-glucose + testosterone | - |
Ogataea angusta DSM 70277 | UDP + testosterone 17-beta-D-glucoside | - |
? | |
| UDP-alpha-D-glucose + testosterone | - |
Kluyveromyces lactis DSM 70799 | UDP + testosterone 17-beta-D-glucoside | - |
? | |
| UDP-alpha-D-glucose + testosterone | - |
Meyerozyma guilliermondii DSM 6381 | UDP + testosterone 17-beta-D-glucoside | - |
? | |
| UDP-alpha-D-glucose + testosterone | - |
Komagataella pastoris GS115 | UDP + testosterone 17-beta-D-glucoside | - |
? | |
| UDP-alpha-D-glucose + testosterone | - |
Saccharomyces cerevisiae ATCC 204508 | UDP + testosterone 17-beta-D-glucoside | - |
? | |
| UDP-alpha-D-glucose + testosterone | - |
Debaryomyces hansenii CBS 767 | UDP + testosterone 17-beta-D-glucoside | - |
? | |
| UDP-glucose + cholesterol | - |
Komagataella pastoris | UDP + cholesterol 3-beta-D-glucoside | - |
? | |
| UDP-glucose + cholesterol | - |
Saccharomyces cerevisiae | UDP + cholesterol 3-beta-D-glucoside | - |
? | |
| UDP-glucose + cholesterol | - |
Meyerozyma guilliermondii | UDP + cholesterol 3-beta-D-glucoside | - |
? | |
| UDP-glucose + cholesterol | - |
Ogataea angusta | UDP + cholesterol 3-beta-D-glucoside | - |
? | |
| UDP-glucose + cholesterol | - |
Kluyveromyces lactis | UDP + cholesterol 3-beta-D-glucoside | - |
? | |
| UDP-glucose + cholesterol | - |
Debaryomyces hansenii | UDP + cholesterol 3-beta-D-glucoside | - |
? | |
| UDP-glucose + cholesterol | - |
Ogataea angusta DSM 70277 | UDP + cholesterol 3-beta-D-glucoside | - |
? | |
| UDP-glucose + cholesterol | - |
Kluyveromyces lactis DSM 70799 | UDP + cholesterol 3-beta-D-glucoside | - |
? | |
| UDP-glucose + cholesterol | - |
Meyerozyma guilliermondii DSM 6381 | UDP + cholesterol 3-beta-D-glucoside | - |
? | |
| UDP-glucose + cholesterol | - |
Komagataella pastoris GS115 | UDP + cholesterol 3-beta-D-glucoside | - |
? | |
| UDP-glucose + cholesterol | - |
Saccharomyces cerevisiae ATCC 204508 | UDP + cholesterol 3-beta-D-glucoside | - |
? | |
| UDP-glucose + cholesterol | - |
Debaryomyces hansenii CBS 767 | UDP + cholesterol 3-beta-D-glucoside | - |
? | |
| UDP-glucose + ergosterol | - |
Komagataella pastoris | UDP + ergosterol 3-beta-D-glucoside | - |
? | |
| UDP-glucose + ergosterol | - |
Saccharomyces cerevisiae | UDP + ergosterol 3-beta-D-glucoside | - |
? | |
| UDP-glucose + ergosterol | - |
Meyerozyma guilliermondii | UDP + ergosterol 3-beta-D-glucoside | - |
? | |
| UDP-glucose + ergosterol | - |
Ogataea angusta | UDP + ergosterol 3-beta-D-glucoside | - |
? | |
| UDP-glucose + ergosterol | - |
Kluyveromyces lactis | UDP + ergosterol 3-beta-D-glucoside | - |
? | |
| UDP-glucose + ergosterol | - |
Debaryomyces hansenii | UDP + ergosterol 3-beta-D-glucoside | - |
? | |
| UDP-glucose + ergosterol | - |
Ogataea angusta DSM 70277 | UDP + ergosterol 3-beta-D-glucoside | - |
? | |
| UDP-glucose + ergosterol | - |
Kluyveromyces lactis DSM 70799 | UDP + ergosterol 3-beta-D-glucoside | - |
? | |
| UDP-glucose + ergosterol | - |
Meyerozyma guilliermondii DSM 6381 | UDP + ergosterol 3-beta-D-glucoside | - |
? | |
| UDP-glucose + ergosterol | - |
Komagataella pastoris GS115 | UDP + ergosterol 3-beta-D-glucoside | - |
? | |
| UDP-glucose + ergosterol | - |
Saccharomyces cerevisiae ATCC 204508 | UDP + ergosterol 3-beta-D-glucoside | - |
? | |
| UDP-glucose + ergosterol | - |
Debaryomyces hansenii CBS 767 | UDP + ergosterol 3-beta-D-glucoside | - |
? | |
| UDP-glucose + estradiol | - |
Komagataella pastoris | UDP + estradiol 3-beta-D-glucoside | - |
? | |
| UDP-glucose + estradiol | - |
Saccharomyces cerevisiae | UDP + estradiol 3-beta-D-glucoside | - |
? | |
| UDP-glucose + estradiol | - |
Meyerozyma guilliermondii | UDP + estradiol 3-beta-D-glucoside | - |
? | |
| UDP-glucose + estradiol | - |
Ogataea angusta | UDP + estradiol 3-beta-D-glucoside | - |
? | |
| UDP-glucose + estradiol | - |
Kluyveromyces lactis | UDP + estradiol 3-beta-D-glucoside | - |
? | |
| UDP-glucose + estradiol | - |
Debaryomyces hansenii | UDP + estradiol 3-beta-D-glucoside | - |
? | |
| UDP-glucose + estradiol | - |
Ogataea angusta DSM 70277 | UDP + estradiol 3-beta-D-glucoside | - |
? | |
| UDP-glucose + estradiol | - |
Kluyveromyces lactis DSM 70799 | UDP + estradiol 3-beta-D-glucoside | - |
? | |
| UDP-glucose + estradiol | - |
Meyerozyma guilliermondii DSM 6381 | UDP + estradiol 3-beta-D-glucoside | - |
? | |
| UDP-glucose + estradiol | - |
Komagataella pastoris GS115 | UDP + estradiol 3-beta-D-glucoside | - |
? | |
| UDP-glucose + estradiol | - |
Saccharomyces cerevisiae ATCC 204508 | UDP + estradiol 3-beta-D-glucoside | - |
? | |
| UDP-glucose + estradiol | - |
Debaryomyces hansenii CBS 767 | UDP + estradiol 3-beta-D-glucoside | - |
? | |
| UDP-glucose + estrone | - |
Komagataella pastoris | UDP + estrone 3-beta-D-glucoside | - |
? | |
| UDP-glucose + estrone | - |
Saccharomyces cerevisiae | UDP + estrone 3-beta-D-glucoside | - |
? | |
| UDP-glucose + estrone | - |
Meyerozyma guilliermondii | UDP + estrone 3-beta-D-glucoside | - |
? | |
| UDP-glucose + estrone | - |
Ogataea angusta | UDP + estrone 3-beta-D-glucoside | - |
? | |
| UDP-glucose + estrone | - |
Kluyveromyces lactis | UDP + estrone 3-beta-D-glucoside | - |
? | |
| UDP-glucose + estrone | - |
Debaryomyces hansenii | UDP + estrone 3-beta-D-glucoside | - |
? | |
| UDP-glucose + estrone | - |
Ogataea angusta DSM 70277 | UDP + estrone 3-beta-D-glucoside | - |
? | |
| UDP-glucose + estrone | - |
Kluyveromyces lactis DSM 70799 | UDP + estrone 3-beta-D-glucoside | - |
? | |
| UDP-glucose + estrone | - |
Meyerozyma guilliermondii DSM 6381 | UDP + estrone 3-beta-D-glucoside | - |
? | |
| UDP-glucose + estrone | - |
Komagataella pastoris GS115 | UDP + estrone 3-beta-D-glucoside | - |
? | |
| UDP-glucose + estrone | - |
Saccharomyces cerevisiae ATCC 204508 | UDP + estrone 3-beta-D-glucoside | - |
? | |
| UDP-glucose + estrone | - |
Debaryomyces hansenii CBS 767 | UDP + estrone 3-beta-D-glucoside | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 54200, about, sequence calculation | Ogataea angusta |
| ? | x * 55900, about, sequence calculation | Kluyveromyces lactis |
| ? | x * 61800, about, sequence calculation | Debaryomyces hansenii |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| UDPG-SGTase | - |
Komagataella pastoris |
| UDPG-SGTase | - |
Saccharomyces cerevisiae |
| UDPG-SGTase | - |
Meyerozyma guilliermondii |
| UDPG-SGTase | - |
Ogataea angusta |
| UDPG-SGTase | - |
Kluyveromyces lactis |
| UDPG-SGTase | - |
Debaryomyces hansenii |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Komagataella pastoris |
| 30 | - |
assay at | Saccharomyces cerevisiae |
| 30 | - |
assay at | Meyerozyma guilliermondii |
| 30 | - |
assay at | Ogataea angusta |
| 30 | - |
assay at | Kluyveromyces lactis |
| 30 | - |
assay at | Debaryomyces hansenii |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 351.9 | - |
testosterone | pH 7.8, 30°C | Ogataea angusta | |
| 475 | - |
cholesterol | pH 7.8, 30°C | Kluyveromyces lactis | |
| 650.6 | - |
cholesterol | pH 7.8, 30°C | Ogataea angusta | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.8 | - |
assay at | Komagataella pastoris |
| 7.8 | - |
assay at | Saccharomyces cerevisiae |
| 7.8 | - |
assay at | Meyerozyma guilliermondii |
| 7.8 | - |
assay at | Ogataea angusta |
| 7.8 | - |
assay at | Kluyveromyces lactis |
| 7.8 | - |
assay at | Debaryomyces hansenii |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00042 | - |
testosterone | pH 7.8, 30°C | Ogataea angusta | |
| 0.00721 | - |
cholesterol | pH 7.8, 30°C | Kluyveromyces lactis | |
| 0.0097 | - |
cholesterol | pH 7.8, 30°C | Ogataea angusta | |
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