Cloned (Comment) | Organism |
---|---|
gene CSGALNACT1, quantitative real-time RT-PCR enzyme expression analysis | Mus musculus |
gene CSGALNACT2, quantitative real-time RT-PCR enzyme expression analysis | Mus musculus |
Protein Variants | Comment | Organism |
---|---|---|
additional information | construction of enzyme knockout mutant ChGn-1-/- | Mus musculus |
additional information | construction of enzyme knockout mutant ChGn-2-/- | Mus musculus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | required | Mus musculus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | Q8BJQ9 | gene csgalnact1 | - |
Mus musculus | Q8C1F4 | gene csgalnact2 | - |
Mus musculus C57BL/6 | Q8BJQ9 | gene csgalnact1 | - |
Mus musculus C57BL/6 | Q8C1F4 | gene csgalnact2 | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
cartilage | growth plate cartilage | Mus musculus | - |
embryo | - |
Mus musculus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | no activity with GlcUAbeta1-3Galbeta1-3Galbeta1-4Xyl(2-O-phosphate)-1-O-thrombomodulin and GlcUAbeta1-3Galbeta1-3Galbeta1-4Xyl(2-O-phosphate) | Mus musculus | ? | - |
? | |
additional information | no activity with GlcUAbeta1-3Galbeta1-3Galbeta1-4Xyl(2-O-phosphate)-1-O-thrombomodulin and GlcUAbeta1-3Galbeta1-3Galbeta1-4Xyl(2-O-phosphate) | Mus musculus C57BL/6 | ? | - |
? | |
UDP-N-acetyl-D-galactosamine + GlcUAbeta1-3Galbeta1-3Galbeta1-4Xyl(2-O-phosphate) | a phosphorylated tetrasaccharide linkage structure in ChGn-1-/- growth plate cartilage but not in ChGn-2-/- or wild-type growth plate cartilage, preferred substrate of ChGn-1 | Mus musculus | UDP + N-acetyl-D-galactosaminyl-1-4beta-D-GlcUAbeta1-3Galbeta1-3Galbeta1-4Xyl(2-O-phosphate) | - |
? | |
UDP-N-acetyl-D-galactosamine + GlcUAbeta1-3Galbeta1-3Galbeta1-4Xyl(2-O-phosphate) | a phosphorylated tetrasaccharide linkage structure in ChGn-1-/- growth plate cartilage but not in ChGn-2-/- or wild-type growth plate cartilage, preferred substrate of ChGn-1 | Mus musculus C57BL/6 | UDP + N-acetyl-D-galactosaminyl-1-4beta-D-GlcUAbeta1-3Galbeta1-3Galbeta1-4Xyl(2-O-phosphate) | - |
? | |
UDP-N-acetyl-D-galactosamine + GlcUAbeta1-3Galbeta1-3Galbeta1-4Xyl(2-O-phosphate)-1-O-thrombomodulin | - |
Mus musculus | UDP + N-acetyl-D-galactosaminyl-1-4beta-D-GlcUAbeta1-3Galbeta1-3Galbeta1-4Xyl(2-O-phosphate)-1-O-thrombomodulin | - |
? | |
UDP-N-acetyl-D-galactosamine + GlcUAbeta1-3Galbeta1-3Galbeta1-4Xyl(2-O-phosphate)-1-O-thrombomodulin | - |
Mus musculus C57BL/6 | UDP + N-acetyl-D-galactosaminyl-1-4beta-D-GlcUAbeta1-3Galbeta1-3Galbeta1-4Xyl(2-O-phosphate)-1-O-thrombomodulin | - |
? |
Synonyms | Comment | Organism |
---|---|---|
ChGn-1 | - |
Mus musculus |
ChGn-2 | - |
Mus musculus |
chondroitin N-acetylgalactosaminyltransferase-1 | - |
Mus musculus |
chondroitin N-acetylgalactosaminyltransferase-2 | - |
Mus musculus |
GalNAcT-I | - |
Mus musculus |
GalNAcT-II | - |
Mus musculus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Mus musculus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.8 | - |
assay at | Mus musculus |
General Information | Comment | Organism |
---|---|---|
malfunction | deficiency in chondroitin N-acetylgalactosaminyltransferase-1 reduces the number of chondroitin sulfate chains, leading to skeletal dysplasias in mice. Accumulation of phosphorylated pentasaccharide and tetrasaccharide linkages in ChGn-1-/- growth plate cartilage | Mus musculus |
additional information | proportion of linkage region saccharides from wild-type, ChGn-1-/-, or ChGn-2-/- cartilage, overview | Mus musculus |
physiological function | chondroitin N-acetylgalactosaminyltransferase-1 regulates the number of chondroitin sulfate chains for normal cartilage development. ChGn-1-mediated addition of N-acetylgalactosamine is accompanied by rapid 2-phosphoxylose phosphatase XYLP-dependent dephosphorylation during formation of the CS linkage region. ChGn-1 and XYLP cooperatively regulate the number of chondroitin sulfate chains in growth plate cartilage | Mus musculus |