Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.4.1.175 extracted from

  • Tsuchida, K.; Lind, T.; Kitagawa, H.; Lindahl, U.; Sugahara, K.; Lidholt, K.
    Purification and characterization of fetal bovine serum beta-N-acetyl-D-galactosaminyltransferase and beta-D-glucuronyltransferase involved in chondroitin sulfate biosynthesis (1999), Eur. J. Biochem., 264, 461-467.
    View publication on PubMed
Search for more literature for 2.4.1.175

General Stability

General Stability Organism
stable against freeze-thawing in liquid N2 several times Bos taurus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
160000
-
 gel filtration Bos taurus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
UDP-N-acetyl-alpha-D-galactosamine + chondroitin oligosaccharide Bos taurus involved in chondroitin biosynthesis UDP + N-acetyl-beta-D-galactosaminyl chondroitin oligosaccharide
-
 ?
UDP-N-acetyl-alpha-D-galactosamine + chondroitin sulfate oligosaccharide Bos taurus involved in chondroitin sulfate biosynthesis: elongation of growing chondroitin sulfate chain UDP + N-acetyl-beta-D-galactosaminyl chondroitin sulfate oligosaccharide
-
 ?

Organism

Organism UniProt Comment Textmining
Bos taurus
-
 fetal
-

Purification (Commentary)

Purification (Comment) Organism
-
 Bos taurus

Source Tissue

Source Tissue Comment Organism Textmining
serum
-
 Bos taurus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.0425
-
 purified enzyme Bos taurus

Storage Stability

Storage Stability Organism
-80°C, MES/NaOH, 50 mM, pH 6.5, 1 mM dithiothreitol, stable for at least a few months Bos taurus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information the enzyme also has the 3-beta-glucuronosyltransferase, EC 2.4.1.226, activity Bos taurus ?
-
 ?
UDP-N-acetyl-alpha-D-galactosamine + (glucuronyl-N-acetyl-1,3-beta-D-galactosamine)3 minimal chain length required for activity Bos taurus UDP + N-acetyl-beta-D-galactosaminyl-1,4-glucuronyl-N-acetyl-1,3-beta-D-galactosaminyl-(glucuronyl-N-acetyl-1,3-beta-D-galactosamine)2
-
 ?
UDP-N-acetyl-alpha-D-galactosamine + chondroitin oligosaccharide involved in chondroitin biosynthesis Bos taurus UDP + N-acetyl-beta-D-galactosaminyl chondroitin oligosaccharide
-
 ?
UDP-N-acetyl-alpha-D-galactosamine + chondroitin sulfate oligosaccharide involved in chondroitin sulfate biosynthesis: elongation of growing chondroitin sulfate chain Bos taurus UDP + N-acetyl-beta-D-galactosaminyl chondroitin sulfate oligosaccharide
-
 ?
UDP-N-acetyl-D-galactosamine + chondroitin oligosaccharide acceptors are even-numbered chondroitin oligosaccharides with terminal glucuronyl-N-acetyl-1,3-beta-D-galactosamine sequence at non-reducing end, transfers N-acetyl-galactosamine to non-reducing glucuronic acid residues in beta-glycosidic linkage Bos taurus UDP + N-acetyl-D-galactosaminyl chondroitin oligosaccharide
-
 ?

Synonyms

Synonyms Comment Organism
More the human form of this enzyme is a bifunctional glycosyltransferase, which also has the 3-beta-glucuronosyltransferase, EC 2.4.1.226, activity required for the synthesis of the chondroitin sulfate disaccharide repeats. Similar chondroitin synthase co-polymerases can be found in Pasteurella multocida and Escherichia coli. Bos taurus
More the enzyme also has the 3-beta-glucuronosyltransferase, EC 2.4.1.226, activity Bos taurus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
 assay at Bos taurus