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Literature summary for 2.4.1.175 extracted from
- Microbial glycosaminoglycan glycosyltransferases (2002), Glycobiology, 12, 9R-16R.
Application
| Application | Comment | Organism |
|---|---|---|
| medicine | antimicrobial approach, understanding microbial capsule biosynthesis may assist the uncloaking of bacterial camouflage without disrupting host GAG synthesis | Escherichia coli |
| medicine | antimicrobial approach, understanding microbial capsule biosynthesis may assist the uncloaking of bacterial camouflage without disrupting host GAG synthesis | Pasteurella multocida |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
- |
Escherichia coli |
- |
Pasteurella multocida |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| additional information | transport of the nascent polymer chain out of the cell through the lipid membranes requires at least seven distinct polypeptide species, possible transport mechanism | Escherichia coli | - |
- |
| additional information | the carboxy-terminal tail is likely to contain a docking segment that interacts with the transport mechanism | Pasteurella multocida | - |
- |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| UDP-N-acetyl-alpha-D-galactosamine + chondroitin oligosaccharide | Escherichia coli | biosynthesis of K4 antigen, a capsule polysaccharide consisting of a chondroitin backbone to which beta-fructose is linked at position C-3 of the GlcNAc residue | UDP + N-acetyl-beta-D-galactosaminyl chondroitin oligosaccharide | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Escherichia coli | - |
- |
- |
| Escherichia coli K4 | - |
- |
- |
| Pasteurella multocida | Q9CMP0 | type F | - |
Reaction
| Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|
| UDP-N-acetyl-alpha-D-galactosamine + [protein]-3-O-(beta-D-GlcA-(1->3)-beta-D-GalNAc-(1->4)-beta-D-GlcA-(1->3)-beta-D-Gal-(1->3)-beta-D-Gal-(1->4)-beta-D-Xyl)-L-serine = UDP + [protein]-3-O-(beta-D-GalNAc-(1->4)-beta-D-GlcA-(1->3)-beta-D-GalNAc-(1->4)-beta-D-GlcA-(1->3)-beta-D-Gal-(1->3)-beta-D-Gal-(1->4)-beta-D-Xyl)-L-serine | enzyme contains 2 distinct catalytic site for GalNAc and GlcNAc transferase activity | Pasteurella multocida |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| UDP-N-acetyl-alpha-D-galactosamine + chondroitin oligosaccharide | biosynthesis of K4 antigen, a capsule polysaccharide consisting of a chondroitin backbone to which beta-fructose is linked at position C-3 of the GlcNAc residue | Escherichia coli | UDP + N-acetyl-beta-D-galactosaminyl chondroitin oligosaccharide | - |
? | |
| UDP-N-acetyl-D-galactosamine + chondroitin oligosaccharide | long polymers are not formed | Escherichia coli | UDP + N-acetyl-D-galactosaminyl chondroitin oligosaccharide | - |
? | |
| UDP-N-acetyl-D-galactosamine + chondroitin oligosaccharide | high specificity for sugar donor substrates | Pasteurella multocida | UDP + N-acetyl-D-galactosaminyl chondroitin oligosaccharide | transfer in beta-1,4-linkage | ? | |
| UDP-N-acetyl-D-galactosamine + chondroitin oligosaccharide | in vitro: addition of single sugars to a nonreducing terminal GlcNAc residue of a chondroitin-derived oligosaccharide acceptor | Escherichia coli | UDP + N-acetyl-D-galactosaminyl chondroitin oligosaccharide | - |
? | |
| UDP-N-acetyl-D-galactosamine + chondroitin oligosaccharide | in vitro: addition of single sugars to a nonreducing terminal GlcNAc residue of a chondroitin-derived oligosaccharide acceptor | Pasteurella multocida | UDP + N-acetyl-D-galactosaminyl chondroitin oligosaccharide | transfer in beta-1,4-linkage | ? | |
| UDP-N-acetyl-D-galactosamine + chondroitin oligosaccharide | long polymers are not formed | Escherichia coli K4 | UDP + N-acetyl-D-galactosaminyl chondroitin oligosaccharide | - |
? | |
| UDP-N-acetyl-D-galactosamine + chondroitin oligosaccharide | in vitro: addition of single sugars to a nonreducing terminal GlcNAc residue of a chondroitin-derived oligosaccharide acceptor | Escherichia coli K4 | UDP + N-acetyl-D-galactosaminyl chondroitin oligosaccharide | - |
? | |
| UDP-N-acetyl-D-galactosamine + chondroitin sulfate C | no activity | Escherichia coli | UDP + N-acetyl-D-galactosaminyl chondroitin sulfate C | - |
? | |
| UDP-N-acetyl-D-galactosamine + chondroitin sulfate C | no activity | Pasteurella multocida | UDP + N-acetyl-D-galactosaminyl chondroitin sulfate C | - |
? | |
| UDP-N-acetyl-D-galactosamine + chondroitin sulfate C | no activity | Escherichia coli K4 | UDP + N-acetyl-D-galactosaminyl chondroitin sulfate C | - |
? | |
| UDP-N-acetyl-D-galactosamine + chondroitin sulfate oligosaccharide | no activity | Escherichia coli | UDP + N-acetyl-D-galactosaminyl chondroitin sulfate oligosaccharide | - |
? | |
| UDP-N-acetyl-D-galactosamine + chondroitin sulfate oligosaccharide | no activity | Pasteurella multocida | UDP + N-acetyl-D-galactosaminyl chondroitin sulfate oligosaccharide | - |
? | |
| UDP-N-acetyl-D-galactosamine + chondroitin sulfate oligosaccharide | no activity | Escherichia coli K4 | UDP + N-acetyl-D-galactosaminyl chondroitin sulfate oligosaccharide | - |
? | |
| UDP-N-acetyl-D-galactosamine + defructosylated K4 oligosaccharide | the native K4 oligosaccharide, containing a fructose residue branch at C-3 of each GlcNAc residue of the chondroitin backbone, is no substrate | Escherichia coli | UDP + N-acetyl-D-galactosaminyl defructosylated K4 oligosaccharide | - |
? | |
| UDP-N-acetyl-D-galactosamine + defructosylated K4 oligosaccharide | in vitro: addition of single sugars to a nonreducing terminal GlcNAc residue of a chondroitin-derived oligosaccharide acceptor, but long polymers are not formed | Escherichia coli | UDP + N-acetyl-D-galactosaminyl defructosylated K4 oligosaccharide | - |
? | |
| UDP-N-acetyl-D-galactosamine + defructosylated K4 oligosaccharide | the native K4 oligosaccharide, containing a fructose residue branch at C-3 of each GlcNAc residue of the chondroitin backbone, is no substrate | Escherichia coli K4 | UDP + N-acetyl-D-galactosaminyl defructosylated K4 oligosaccharide | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| More | the human form of this enzyme is a bifunctional glycosyltransferase, which also has the 3-beta-glucuronosyltransferase, EC 2.4.1.226, activity required for the synthesis of the chondroitin sulfate disaccharide repeats. Similar chondroitin synthase co-polymerases can be found in Pasteurella multocida and Escherichia coli. | Pasteurella multocida |
| More | the enzyme also has the 3-beta-glucuronosyltransferase, EC 2.4.1.226, activity | Pasteurella multocida |
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