Cloned (Comment) | Organism |
---|---|
ChGn-2, expression analysis by quantitative real-time RT-PCR, co-expression of ChGn-1 or ChGn-2 into sog9 and L cells, sog9 is a mutant L cell line are deficient in the expression of chondroitin 4-O-sulfotransferase-1, C4ST-1, disaccharide composition of chondroitin sulfate in control and transfected L cells, overview | Mus musculus |
Protein Variants | Comment | Organism |
---|---|---|
D367A | site-directed mutagenesis, a soluble mutant is generated by replacing the first 36 amino acids of ChGn-2 D367A with a cleavable insulin signal sequence and the protein A IgG-binding domain, inactive mutant | Mus musculus |
additional information | silencing of ChGn-2 in L cells by shRNA | Mus musculus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | - |
- |
- |
Synonyms | Comment | Organism |
---|---|---|
ChGn-2 | - |
Mus musculus |
chondroitin N-acetylgalactosaminyltransferase-2 | - |
Mus musculus |
General Information | Comment | Organism |
---|---|---|
malfunction | overexpression of ChGn-2 increases the length and amount of chondroitin sulfate chains in L cells, but not in sog9 mutant cells. Knockdown of ChGn-2 results in a decrease in the amount of CS in L cells in a manner proportional to ChGn-2 expression levels, whereas the introduction of mutated ChGn-2 lacking enzyme activity fails to increase the amount of chondroitin sulfate | Mus musculus |
physiological function | chondroitin 4-O-sulfotransferase-1, C4ST-1 regulates the chain length and amount of chondroitin sulfate in co-operation with ChGn-2 the enzymes play a critical role in chondroitin sulfate chain elongation | Mus musculus |