Cloned (Comment) | Organism |
---|---|
gene Sbe2a, recombinant expression of N-terminally His6-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)pLysS | Zea mays |
Protein Variants | Comment | Organism |
---|---|---|
E513D | site-directed mutagenesis, the mutant shows a much lower activity compared to wild-type enzyme mSBEIIa | Zea mays |
additional information | construction of enzyme point mutants by site-directed mutagenesis to change the chain-length distribution CLD by changing activity of enzyme SBE. The enzyme mutants show no or only a slight change in degree of polymerization of branched glucans | Zea mays |
R363K | site-directed mutagenesis, the mutant shows similar activity as the wild-type enzyme mSBEIIa | Zea mays |
R456K | site-directed mutagenesis, the mutant shows similar activity compared to wild-type enzyme mSBEIIa | Zea mays |
S349F | site-directed mutagenesis, creates an additional binding site for glucose, the mutant shows a much lower activity compared to wild-type enzyme mSBEIIa | Zea mays |
Y352F | site-directed mutagenesis, , the mutant shows a much lower activity compared to wild-type enzyme mSBEIIa | Zea mays |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Zea mays | O24421 | isozyme SBEIIa | - |
Purification (Comment) | Organism |
---|---|
recombinant N-terminally His6-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3)pLysS by nickel affinity chromatography | Zea mays |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | molecular weight distribution of starch, amylose and amylopectin, overview. SBE is the only enzyme that generates glucan branches, i.e. it is the only chain-stopping substance for branch growth, and as a consequence SBE has a significant effect on the final structure of the resulting starch | Zea mays | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
SBE | - |
Zea mays |
SBEIIA | - |
Zea mays |
starch branching enzyme | - |
Zea mays |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Zea mays |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Zea mays |
General Information | Comment | Organism |
---|---|---|
additional information | Tyr352, Glu513, and Ser349 are important for mSBEIIa activity while Arg456 is important for determining the position at which the linear glucan is cut, enzyme active site structure, molecular dynamics simulations and modeling, overview | Zea mays |
physiological function | SBE is the only enzyme that generates glucan branches, i.e. it is the only chain-stopping substance for branch growth, and as a consequence SBE has a significant effect on the final structure of the resulting starch | Zea mays |