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Literature summary for 2.4.1.186 extracted from

  • Carrizo, M.E.; Miozzo, M.C.; Goldraij, A.; Curtino, J.A.
    Purification of rabbit skeletal muscle proteoglycogen: studies on the glucosyltransferase activity of polysaccharide-free and -bound glycogenin (1997), Glycobiology, 7, 571-578.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
UDP inhibits both autoglucosylation and glucosylation of exogenous acceptor Oryctolagus cuniculus

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+ dependent on, also required for transglucosylation Oryctolagus cuniculus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
200000
-
gel filtration, active proteoglycogen Oryctolagus cuniculus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
UDP-glucose + glycogenin Oryctolagus cuniculus enzyme forms the protein part of proteoglycogen UDP + glucosylated glycogenin
-
?

Organism

Organism UniProt Comment Textmining
Oryctolagus cuniculus
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein autoglycosylation Oryctolagus cuniculus

Purification (Commentary)

Purification (Comment) Organism
separation from proteoglycogen Oryctolagus cuniculus

Source Tissue

Source Tissue Comment Organism Textmining
skeletal muscle
-
Oryctolagus cuniculus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Oryctolagus cuniculus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information no activity with nonapeptide SISIYSYLP and N-lactosylated peptide Oryctolagus cuniculus ?
-
?
UDP-galactose + glycogenin autoglycosylation reaction Oryctolagus cuniculus UDP + galactosylated glycogenin
-
?
UDP-glucose + glycogenin autoglycosylation reaction Oryctolagus cuniculus UDP + glucosylated glycogenin
-
?
UDP-glucose + glycogenin enzyme forms the protein part of proteoglycogen Oryctolagus cuniculus UDP + glucosylated glycogenin
-
?
UDP-glucose + glycogenin no activity with UDP-N-acetylglucosamine and GDP-mannose Oryctolagus cuniculus UDP + glucosylated glycogenin
-
?
UDP-glucose + N-(maltosyl-alpha-1,4-(1-deoxyglucitol))-peptide simultaneously and independently of the autoglycosylation reaction Oryctolagus cuniculus UDP + glucosylated N-(maltosyl-alpha-1,4-(1-deoxyglucitol))-peptide
-
?
UDP-glucose + N-(maltosyl-alpha-1,4-(1-deoxyglucitol))-peptide peptide sequence: SISIYSYLP Oryctolagus cuniculus UDP + glucosylated N-(maltosyl-alpha-1,4-(1-deoxyglucitol))-peptide
-
?
UDP-glucose + n-dodecyl-beta-D-maltoside simultaneously and independently of the autoglycosylation reaction Oryctolagus cuniculus UDP + n-dodecyl-beta-D-maltotriose
-
?

Subunits

Subunits Comment Organism
More enzyme forms the protein part of proteoglycogen Oryctolagus cuniculus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Oryctolagus cuniculus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
assay at Oryctolagus cuniculus