Cloned (Comment) | Organism |
---|---|
expression of mutant Y194F in Escherichia coli | Oryctolagus cuniculus |
functional expression of the wild-type enzyme in Escherichia coli | Oryctolagus cuniculus |
Protein Variants | Comment | Organism |
---|---|---|
Y194F | exchange of glucose attachment site, no autoglucosylation activity | Rattus norvegicus |
Y194F | exchange of glucose attachment site, no autoglucosylation activity | Oryctolagus cuniculus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | - |
Oryctolagus cuniculus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
- |
- |
Oryctolagus cuniculus | - |
- |
- |
Rattus norvegicus | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | 1 single glucose attachment site at tyrosine-194 | Oryctolagus cuniculus |
glycoprotein | autoglycosylation | Rattus norvegicus |
glycoprotein | autoglycosylation | Oryctolagus cuniculus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
UDP-alpha-D-glucose + glycogenin = UDP + alpha-D-glucosylglycogenin | initiation of glycogen biosynthesis is a 2-step mechanism, requiring first the covalent attachment of a glucose residue to Tyr-194 of glycogenin and then elongation to form an oligosaccharide chain | Oryctolagus cuniculus | |
UDP-alpha-D-glucose + glycogenin = UDP + alpha-D-glucosylglycogenin | highly conserved protein | Rattus norvegicus | |
UDP-alpha-D-glucose + glycogenin = UDP + alpha-D-glucosylglycogenin | highly conserved protein | Bos taurus | |
UDP-alpha-D-glucose + glycogenin = UDP + alpha-D-glucosylglycogenin | highly conserved protein | Oryctolagus cuniculus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
kidney | - |
Rattus norvegicus | - |
kidney | - |
Bos taurus | - |
kidney | low content | Oryctolagus cuniculus | - |
liver | - |
Rattus norvegicus | - |
liver | very low content | Oryctolagus cuniculus | - |
skeletal muscle | - |
Rattus norvegicus | - |
skeletal muscle | - |
Bos taurus | - |
skeletal muscle | - |
Oryctolagus cuniculus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-glucose + glycogenin | autoglycosylation reaction | Rattus norvegicus | UDP + glucosylated glycogenin | - |
? | |
UDP-glucose + glycogenin | autoglycosylation reaction | Bos taurus | UDP + glucosylated glycogenin | - |
? | |
UDP-glucose + glycogenin | autoglycosylation reaction | Oryctolagus cuniculus | UDP + glucosylated glycogenin | glucose molecule is attached to Tyr-194 | ? |