Literature summary for 2.4.1.186 extracted from

Hurley, T.D.; Walls, C.; Bennett, J.R.; Roach, P.J.; Wang, M.
Direct detection of glycogenin reaction products during glycogen initiation (2006), Biochem. Biophys. Res. Commun., 348, 374-378.

Search for more literature for 2.4.1.186

Cloned(Commentary)

Cloned (Comment)	Organism
expresssion of recombinant protein in the CGSC Escherichia coli cell line 4997, which lacks UDP-glucose pyrophosphorylase activity	Oryctolagus cuniculus

General Stability

General Stability	Organism
concentrated to 8 mg/ml, and 100 microl aliquots are flash frozen in liquid nitrogen and stored at -80°C. Glycogenin stored in this manner is stable for at least 2 years.	Oryctolagus cuniculus

Organism

Organism	UniProt	Comment	Textmining
Oryctolagus cuniculus	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
nickel-NTA column and Q-Sepharose column	Oryctolagus cuniculus

Source Tissue

Source Tissue	Comment	Organism	Textmining
muscle	-	Oryctolagus cuniculus	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
UDP-alpha-D-glucose + glycogenin	-	Oryctolagus cuniculus	additional information	after 60 min of incubation the glycogenin molecules possess an average glucosyl chain length of 11.3 residues	?

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
additional information	-	additional information	the catalytic effectiveness of glycogenin falls off dramatically as the average glucosyl chain length increases	Oryctolagus cuniculus
0.0053	-	UDP-glucose	glucosylated protein, 14C-glucose incorporation assay	Oryctolagus cuniculus
0.0083	-	UDP-glucose	glucosylated protein, 4-10 interval, mass sprectrometry assay	Oryctolagus cuniculus
0.0083	-	UDP-glucose	non-glucosylated protein, 14C-glucose incorporation assay	Oryctolagus cuniculus
0.0183	-	UDP-glucose	non-glucosylated protein, mass sprectrometry assay	Oryctolagus cuniculus

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Release 2023.1 (January 2023)