Cloned (Comment) | Organism |
---|---|
expression of wild-type gycogenin-1, its truncated mutant DELTA-270, and of glycogenin-1 point mutants in Escherichia coli strain CGSC 4997 | Oryctolagus cuniculus |
Crystallization (Comment) | Organism |
---|---|
purified recombinant glycogenin-1 truncation mutant DELTA-270, hanging drop vapor diffusion method, the protein in 12% w/v, PEG monomethyl ester 5000, 0.1 M MES, pH 6.5, and 0.2 M ammonium sulfate, is mixed with 1 M ammonium sulfate and 0.1 M sodium phosphate, pH 6.7, 4°C, crystals are soaked in mother liquor containing 1 mM MnSO4 and 1 mM UDP or UDP-glucose, X-ray diffraction structure determination and analysis | Oryctolagus cuniculus |
Protein Variants | Comment | Organism |
---|---|---|
T82M | inactive mutant, the mutation is equivalent to T83M according to previous authors amino acid numbering, it causes glycogenosis showing the loss of Thr82 hydrogen bond to Asp162, the residue involved in the activation step of the glucose transfer reaction mechanism. Autoglucosylation, maltoside transglucosylation and UDP-glucose hydrolyzing activities are abolished | Oryctolagus cuniculus |
T83S | site-directed mutagenesis, the mutant is catalytically active | Oryctolagus cuniculus |
T83V | site-directed mutagenesis, inactive mutant | Oryctolagus cuniculus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mn2+ | required | Oryctolagus cuniculus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-alpha-D-glucose + glycogenin | Oryctolagus cuniculus | autoglucosylation by glycogenin-1 | UDP + alpha-D-glucosylglycogenin | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Oryctolagus cuniculus | P13280 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant wild-type gycogenin-1, its truncated mutant DELTA-270, and of glycogenin-1 point mutants from Escherichia coli strain CGSC 4997 | Oryctolagus cuniculus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-alpha-D-glucose + glycogenin | autoglucosylation by glycogenin-1 | Oryctolagus cuniculus | UDP + alpha-D-glucosylglycogenin | - |
? |
Synonyms | Comment | Organism |
---|---|---|
glycogenin-1 | - |
Oryctolagus cuniculus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Oryctolagus cuniculus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Oryctolagus cuniculus |
General Information | Comment | Organism |
---|---|---|
malfunction | the glycogenin-1 mutation T82M causes glycogenosis. Substitution of Thr82 for serine but not for valine restores the maximum extent of autoglucosylation as well as transglucosylation and UDP-glucose hydrolysis rate, structure analysis, overview | Oryctolagus cuniculus |
physiological function | Asp162 is the residue involved in the activation step of the glucose transfer reaction mechanism | Oryctolagus cuniculus |