Cloned (Comment) | Organism |
---|---|
gene GYG1, recombinant expression of functional GST-tagged human muscle glycogen synthase in complex with human glycogenin-1 in Spodoptera frugiperda Sf9 cells using a bicistronic pFastBac-Dual expression vector and baculovirus transfection | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | P46976 | gene GYG1 | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
glycoprotein | the degree of glycosylation of the enzyme is modest | Homo sapiens |
Purification (Comment) | Organism |
---|---|
recombinant GST-tagged glycogen synthase in complex with glycogenin-1 from Sf9 insect cells by glutathione affinity chromatography and gel filtration | Homo sapiens |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
skeletal muscle | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
UDP-alpha-D-glucose + glycogenin-1 | self-glucosylation of the glycosyltransferase glycogenin-1 | Homo sapiens | UDP + alpha-D-glucosylglycogenin-1 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
glycogenin-1 | - |
Homo sapiens |
GN1 | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Homo sapiens |
General Information | Comment | Organism |
---|---|---|
additional information | the recombinant glycogen synthase-glycogenin-1 complex GYS1:GN1 is functional and exhibits both allosteric and phospho-dependent regulatio, activation of GYS1:GN1 complex by GYS1 dephosphorylationn | Homo sapiens |
physiological function | glycogenin-1 is a constitutively active enzyme so does not represent a point of regulation | Homo sapiens |